DOI: 10.1046/j.1365-313x.2000.00724.x. Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants

Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants

10.1046/j.1365-313x.2000.00724.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryThe chelation of Fe2+ and Mg2+ ions forms protoheme IX and Mg‐protoporphyrin IX, respectively, and the latter is an intermediate in chlorophyll synthesis. Active magnesium protoporphyrin IX chelatase (Mg‐chelatase) is an enzyme complex consisting of three different subunits. To investigate the function of the CHL I subunit of Mg‐chelatase and the effects of modified Mg‐chelatase activity on the tetrapyrrole biosynthetic pathway, we characterized N. tabacum transformants carrying gene constructs with the Chl I cDNA sequence in antisense and sense orientation under the control of the CaMV 35S promoter. Both elevated and diminished levels of Chl I mRNA and Chl I protein led to reduced Mg‐chelatase activities, reflecting a perturbation of the assembly of the enzyme complex. The transformed plants did not accumulate the substrate of Mg‐chelatase, protoporphyrin IX, but the leaves contained less chlorophyll and possessed increased chlorophyll a/b ratios, as well as a deficiency of light‐harvesting chlorophyll binding proteins of photosystems I and II. The expression and activity of several tetrapyrrolic enzymes were reduced in parallel to lower the Mg‐chelatase activity. Consistent with the lower chlorophyll contents, the rate‐limiting synthesis of 5‐aminolevulinate was also decreased in the transgenic lines analyzed. The consequence of reduced Mg‐chelatase on early and late steps of chlorophyll synthesis, and on the organization of light harvesting complexes is discussed.

Bibliography

Papenbrock, J., PfÃ¼ndel, E., Mock, H., & Grimm, B. (2000). Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants. The Plant Journal, 22(2), 155â164. Portico.

Authors 4

Jutta Papenbrock (first)
Erhard Pfündel (additional)
Hans‐Peter Mock (additional)
Bernhard Grimm (additional)

References 45 Referenced 108

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 7:03 a.m.)
Indexed	4 weeks ago (Aug. 6, 2025, 9:19 a.m.)
Issued	25 years, 5 months ago (April 1, 2000)
Published	25 years, 5 months ago (April 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 5 months ago (April 1, 2000)

Funders 0

None

BibTeX

@article{Papenbrock_2000, title={Decreased and increased expression of the subunit CHL I diminishes Mg chelatase activity and reduces chlorophyll synthesis in transgenic tobacco plants}, volume={22}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2000.00724.x}, DOI={10.1046/j.1365-313x.2000.00724.x}, number={2}, journal={The Plant Journal}, publisher={Wiley}, author={Papenbrock, Jutta and Pfündel, Erhard and Mock, Hans‐Peter and Grimm, Bernhard}, year={2000}, month=apr, pages={155–164} }

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