DOI: 10.1046/j.1365-313x.2000.00672.x. Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana

Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana

10.1046/j.1365-313x.2000.00672.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryChlorophyll (Chl) biosynthesis and degradation are the only biochemical processes on Earth that can be directly observed from satellites or other planets. The bulk of the Chls is found in the light‐harvesting antenna complexes of photosynthetic organisms. Surprisingly little is known about the biosynthesis of Chl b, which is the second most abundant Chl pigment after Chl a. We describe here the expression and properties of the chlorophyllide a oxygenase gene (CAO) from Arabidopsis thaliana, which is apparently the key enzyme in Chl b biosynthesis. The recombinant enzyme produced in Escherichia coli catalyses an unusual two‐step oxygenase reaction that is the ‘missing link’ in the chlorophyll cycle of higher plants.

Bibliography

Oster, U., Tanaka, R., Tanaka, A., & RÃ¼diger, W. (2000). Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana. The Plant Journal, 21(3), 305â310. Portico.

Authors 4

Ulrike Oster (first)
Ryouichi Tanaka (additional)
Ayumi Tanaka (additional)
Wolfhart Rüdiger (additional)

References 24 Referenced 242

{'key': 'e_1_2_6_2_1', 'first-page': '1194', 'article-title': 'In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants', 'volume': '316', 'author': 'Bechtold N.', 'year': '1993', 'journal-title': 'C. R. Acad. Sci. Paris, Life Sci.'} / C. R. Acad. Sci. Paris, Life Sci. / In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants by Bechtold N. (1993)
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{'key': 'e_1_2_6_9_1', 'first-page': '1081', 'article-title': 'Transit peptides of thylakoid luminal proteins: the sites of processing are conserved among higher plants', 'volume': '34', 'author': 'Konishi T.', 'year': '1993', 'journal-title': 'Plant Cell Physiol.'} / Plant Cell Physiol. / Transit peptides of thylakoid luminal proteins: the sites of processing are conserved among higher plants by Konishi T. (1993)
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10.1104/pp.113.1.137 / Plant Physiol. / Conversion of chlorophyll b to chlorophyll a and the assembly of chlorophyll with apoproteins by isolated chloroplasts by Ohtsuka T. (1997)
10.1128/MMBR.63.1.106-127.1999 / Microbiol. Mol. Biol. Rev. / Prochloroccus, a marine photosynthetic prokaryote of global significance by Partensky F. (1999)
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10.1038/22101

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5 p.m.)
Deposited	1 year, 9 months ago (Oct. 29, 2023, 7:52 a.m.)
Indexed	18 minutes ago (Aug. 25, 2025, 5:01 p.m.)
Issued	25 years, 6 months ago (Feb. 1, 2000)
Published	25 years, 6 months ago (Feb. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 6 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Oster_2000, title={Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana}, volume={21}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2000.00672.x}, DOI={10.1046/j.1365-313x.2000.00672.x}, number={3}, journal={The Plant Journal}, publisher={Wiley}, author={Oster, Ulrike and Tanaka, Ryouichi and Tanaka, Ayumi and Rüdiger, Wolfhart}, year={2000}, month=feb, pages={305–310} }

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