DOI: 10.1046/j.1365-313x.2000.00653.x. Post‐transcriptional defects in tobacco chloroplast mutants lacking the cytochrome <i>b<sub>6</sub>/f </i>complex

Post‐transcriptional defects in tobacco chloroplast mutants lacking the cytochrome b6/f complex

10.1046/j.1365-313x.2000.00653.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryA variety of post‐transcriptional mechanisms govern the synthesis and assembly of photosynthetic protein complexes in chloroplasts. To test whether such mechanisms are conserved between photosynthetic algae and vascular plants, we have interrupted the chloroplast petA, petB and petD genes of tobacco, which encode three subunits of the cytochrome b6/f complex, and compared our results to those previously obtained with Chlamydomonas reinhardtii. As expected, the mutants exhibited high chlorophyll fluorescence, consistent with the loss of a functional cytochrome b6/f complex. Unlike the corresponding mutants of Chlamydomonas, however, cytochrome f was barely detectable in the ΔpetB or ΔpetD mutants. The amounts of petB‐ and petD‐containing mRNAs were reduced in the mutants compared to wild‐type plants, but the remaining mRNA was normally associated with polysomes. In contrast, there was a decrease in polysome association of the polycistronic petA mRNA in the ΔpetB and ΔpetD mutants, suggesting that the synthesis of cytochrome f may be decreased in the absence of cytochrome b6 or SUIV. These results are discussed in light of the translational autoregulation model that has been proposed for cytochrome b6/f complex assembly in Chlamydomonas.

Bibliography

Monde, R., Zito, F., Olive, J., Wollman, F., & Stern, D. B. (2000). Postâtranscriptional defects in tobacco chloroplast mutants lacking the cytochrome b6/f complex. The Plant Journal, 21(1), 61â72. Portico.

Authors 5

Rita‐Ann Monde (first)
Francesca Zito (additional)
Jacqueline Olive (additional)
Francis‐André Wollman (additional)
David B. Stern (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 4:59 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 7:32 a.m.)
Indexed	1 day, 5 hours ago (Aug. 29, 2025, 6:47 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Monde_2000, title={Post‐transcriptional defects in tobacco chloroplast mutants lacking the cytochrome b6/f complex}, volume={21}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2000.00653.x}, DOI={10.1046/j.1365-313x.2000.00653.x}, number={1}, journal={The Plant Journal}, publisher={Wiley}, author={Monde, Rita‐Ann and Zito, Francesca and Olive, Jacqueline and Wollman, Francis‐André and Stern, David B.}, year={2000}, month=jan, pages={61–72} }

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  "relation": {},
  "ISSN": [
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    "1365-313X"
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  "subject": [],
  "container-title-short": "The Plant Journal",
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}