The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway
10.1046/j.1365-313x.2003.01889.x
Crossref journal-article
Wiley
The Plant Journal (311)
Abstract

SummaryThe Arabidopsis thalianachlorophyll a/b binding protein underexpressed (cue1) mutant, which has been isolated in a screen for chlorophyll a/b binding protein (CAB) underexpressors, exhibits a reticulate leaf phenotype combined with delayed chloroplast development and aberrant shape of the palisade parenchyma cells. The affected gene in cue1 is a phosphoenolpyruvate (PEP)/phosphate translocator (PPT) of the plastid inner envelope membrane. The proposed function of the PPT in C3‐plants is the import of PEP into the stroma as one of the substrates for the shikimate pathway, from which aromatic amino acids and a variety of secondary plant products derive. The mutant phenotype could be: (i) complemented by constitutive overexpression of a heterologous PPT from cauliflower; and (ii) rescued by overexpression of a C4‐type pyruvate,orthophosphate dikinase (PPDK). The latter approach indicates that PEP deficiency within plastids triggers developmental constraints in cue1. The impact of the mutation on aspects of primary and secondary metabolism was assessed in cue1 as well as in the individual transformant lines. The majority of the data obtained in this and an accompanying paper suggest that the mutant phenotype is not simply caused by a general restriction of the shikimate pathway because of a defect in a PPT.

Bibliography

Voll, L., Häusler, R. E., Hecker, R., Weber, A., Weissenböck, G., Fiene, G., Waffenschmidt, S., & Flügge, U. (2003). The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway. The Plant Journal, 36(3), 301–317. Portico.

Authors 8
  1. Lars Voll (first)
  2. Rainer E. Häusler (additional)
  3. Rolf Hecker (additional)
  4. Andreas Weber (additional)
  5. Gottfried Weissenböck (additional)
  6. Gabriele Fiene (additional)
  7. Sabine Waffenschmidt (additional)
  8. Ulf‐Ingo Flügge (additional)
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Dates
Type When
Created 21 years, 10 months ago (Oct. 22, 2003, 4:24 a.m.)
Deposited 1 year, 10 months ago (Oct. 13, 2023, 10:53 a.m.)
Indexed 2 days, 12 hours ago (Aug. 31, 2025, 6:03 a.m.)
Issued 21 years, 10 months ago (Oct. 16, 2003)
Published 21 years, 10 months ago (Oct. 16, 2003)
Published Online 21 years, 10 months ago (Oct. 16, 2003)
Published Print 21 years, 10 months ago (Nov. 1, 2003)
Funders 0

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@article{Voll_2003, title={The phenotype of the Arabidopsis cue1 mutant is not simply caused by a general restriction of the shikimate pathway}, volume={36}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2003.01889.x}, DOI={10.1046/j.1365-313x.2003.01889.x}, number={3}, journal={The Plant Journal}, publisher={Wiley}, author={Voll, Lars and Häusler, Rainer E. and Hecker, Rolf and Weber, Andreas and Weissenböck, Gottfried and Fiene, Gabriele and Waffenschmidt, Sabine and Flügge, Ulf‐Ingo}, year={2003}, month=oct, pages={301–317} }