DOI: 10.1046/j.1365-313x.2003.01795.x. The lower cell density of leaf parenchyma in the Arabidopsis thaliana mutant lcd1‐1 is associated with increased sensitivity to ozone and virulent Pseudomonas syringae

The lower cell density of leaf parenchyma in the Arabidopsis thaliana mutant lcd1‐1 is associated with increased sensitivity to ozone and virulent Pseudomonas syringae

10.1046/j.1365-313x.2003.01795.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryUnder optimal growth conditions (120 µmol photons m−2 sec−1 photosynthetically active radiation (PAR), 16‐h photoperiod), the recessive ozone‐sensitive Arabidopsis thaliana L. Heynh. mutant lcd1‐1 exhibits a pale phenotype compared to the wild type. Confocal and multiphoton microscopy revealed that the paleness of lcd1‐1 is because of a lower cell density in the leaf palisade parenchyma, resulting in decreased chlorophyll content. When exposed to ozone, lcd1‐1 leaves become paler and contain an increased amount of the lipid peroxidation product malondialdehyde compared to the wild type, suggesting that lcd1‐1 suffers from elevated levels of reactive oxygen species (ROS) generated in the apoplast. Infection of leaves with virulent Pseudomonas syringae reveals higher bacterial growth as well as lower pathogenesis‐related protein 1 (PR‐1) and PR‐5 expression in lcd1‐1 than in the wild type. When the wild type and lcd1‐1 are exposed to short‐term high‐light stress, leaves do not bleach in lcd1‐1 and potential activities of photosystems I (PSI) and II (PSII) decrease to a similar extent in both the genotypes, indicating that the photosynthetic apparatus is not affected by lcd1‐1 mutation. The LCD1 gene, found to contain a nonsense mutation in the mutant, has been identified. It is located at the bottom of chromosome 2 of the Arabidopsis genome. However, the function of the protein encoded by LCD1 is not yet known. We hypothesize that LCD1 plays a role in normal leaf development, and that the increased sensitivity to ozone and virulent P. syringae is a secondary effect that presumably results from the lower‐cell‐density phenotype in lcd1‐1.

Bibliography

Barth, C., & Conklin, P. L. (2003). The lower cell density of leaf parenchyma in the Arabidopsis thaliana mutant lcd1â1 is associated with increased sensitivity to ozone and virulent Pseudomonas syringae. The Plant Journal, 35(2), 206â218. Portico.

Authors 2

Carina Barth (first)
Patricia L. Conklin (additional)

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Dates

Type	When
Created	22 years, 1 month ago (July 9, 2003, 5:04 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 12:27 p.m.)
Indexed	4 months, 1 week ago (April 23, 2025, 12:45 p.m.)
Issued	22 years, 2 months ago (June 20, 2003)
Published	22 years, 2 months ago (June 20, 2003)
Published Online	22 years, 2 months ago (June 20, 2003)
Published Print	22 years, 2 months ago (July 1, 2003)

Funders 0

None

BibTeX

@article{Barth_2003, title={The lower cell density of leaf parenchyma in the Arabidopsis thaliana mutant lcd1‐1 is associated with increased sensitivity to ozone and virulent Pseudomonas syringae}, volume={35}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2003.01795.x}, DOI={10.1046/j.1365-313x.2003.01795.x}, number={2}, journal={The Plant Journal}, publisher={Wiley}, author={Barth, Carina and Conklin, Patricia L.}, year={2003}, month=jun, pages={206–218} }

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