DOI: 10.1046/j.1365-313x.2002.01488.x. The <i>HAT2</i> gene, a member of the HD‐Zip gene family, isolated as an auxin inducible gene by DNA microarray screening, affects auxin response in <i>Arabidopsis</i>

The HAT2 gene, a member of the HD‐Zip gene family, isolated as an auxin inducible gene by DNA microarray screening, affects auxin response in Arabidopsis

10.1046/j.1365-313x.2002.01488.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryThe plant hormone, auxin, regulates many aspects of growth and development. Despite its importance, the molecular mechanisms underlying the action of auxin are largely unknown. To gain a more comprehensive understanding of the primary responses to auxin, we analyzed the expression of genes in Arabidopsis seedlings treated with indole‐3‐acetic acid (IAA) for 15 min. We identified a single gene that is downregulated early, and 29 genes that are upregulated early. Several types of typical transcription factors are identified as early upregulated genes, suggesting that auxin signals are mediated by a master set of diverse transcriptional regulators. Of the genes that responded to auxin, the expression of the homeobox gene, HAT2, was induced rapidly. Furthermore, we show that the expression of HAT2 is induced by auxin, but not by other phytohormones. To analyze the function of HAT2 in the plant's response to auxin, we generated 35S::HAT2 transgenic plants. These produced long hypocotyls, epinastic cotyledons, long petioles, and small leaves, which are characteristic of the phenotypes of the auxin‐overproducing mutants, superroot1 (sur1) and superroot2 (sur2). On the other hand, 35S::HAT2 plants showed reduced lateral root elongation, and reduced auxin sensitivity compared to wild‐type plants. Together with the results of RNA blotting and biochemical analyses, these findings suggest that HAT2 plays opposite roles in the shoot and root tissues in regulating auxin‐mediated morphogenesis.

Bibliography

Sawa, S., Ohgishi, M., Goda, H., Higuchi, K., Shimada, Y., Yoshida, S., & Koshiba, T. (2002). The HAT2 gene, a member of the HDâZip gene family, isolated as an auxin inducible gene by DNA microarray screening, affects auxin response in Arabidopsis. The Plant Journal, 32(6), 1011â1022. Portico.

Authors 7

Shinichiro Sawa (first)
Maki Ohgishi (additional)
Hideki Goda (additional)
Kanako Higuchi (additional)
Yukihisa Shimada (additional)
Shigeo Yoshida (additional)
Tomokazu Koshiba (additional)

References 37 Referenced 172

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{'key': 'e_1_2_6_4_1', 'first-page': '1405', 'article-title': 'Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction', 'volume': '7', 'author': 'Boerjan W.', 'year': '1995', 'journal-title': 'Plant Cell'} / Plant Cell / Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction by Boerjan W. (1995)
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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 5:08 p.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 5:43 a.m.)
Indexed	1 week, 4 days ago (Aug. 12, 2025, 6:03 p.m.)
Issued	22 years, 8 months ago (Dec. 1, 2002)
Published	22 years, 8 months ago (Dec. 1, 2002)
Published Online	22 years, 8 months ago (Dec. 20, 2002)
Published Print	22 years, 8 months ago (Dec. 1, 2002)

Funders 0

None

BibTeX

@article{Sawa_2002, title={The HAT2 gene, a member of the HD‐Zip gene family, isolated as an auxin inducible gene by DNA microarray screening, affects auxin response in Arabidopsis}, volume={32}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.2002.01488.x}, DOI={10.1046/j.1365-313x.2002.01488.x}, number={6}, journal={The Plant Journal}, publisher={Wiley}, author={Sawa, Shinichiro and Ohgishi, Maki and Goda, Hideki and Higuchi, Kanako and Shimada, Yukihisa and Yoshida, Shigeo and Koshiba, Tomokazu}, year={2002}, month=dec, pages={1011–1022} }

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