DOI: 10.1046/j.1365-313x.1999.00520.x. Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation

Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation

10.1046/j.1365-313x.1999.00520.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryThe visual marker GUS has been utilized in this study to understand the Arabidopsis thaliana vacuum infiltration transformation process by Agrobacterium tumefaciens. High transformation frequencies of up to 394 transgenic seeds per infiltrated plant were achieved. The results showed that the majority of the transgenic seeds from single infiltrated plants were from independent transformation events based on Southern analysis, progeny segregation, distribution of transgenic seeds throughout the infiltrated plants and the microscopic analysis of GUS expression in ovules of infiltrated plants. GUS expression in mature pollen and anthers was monitored daily from 0 to 12 days post‐infiltration. In addition, all ovules from a single infiltrated plant were examined every other day. GUS expression frequencies of up to 1% of pollen were observed 3–5 days post‐infiltration, whereas frequencies of up to 6% were detected with ovules of unopened flowers 5–11 days post‐infiltration. Most importantly, transgenic seeds were obtained only from genetic crosses using infiltrated plants as the pollen recipient but not the pollen donor, demonstrating Agrobacterium transformation through the ovule pathway.

Bibliography

Ye, G., Stone, D., Pang, S., Creely, W., Gonzalez, K., & Hinchee, M. (1999). Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation. The Plant Journal, 19(3), 249â257. Portico.

Authors 6

Guang‐Ning Ye (first)
Deborah Stone (additional)
Sheng‐Zhi Pang (additional)
Wendi Creely (additional)
Kathleen Gonzalez (additional)
Maud Hinchee (additional)

References 22 Referenced 134

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10.1046/j.1365-313X.1994.5040551.x
10.1046/j.1365-313x.1998.00343.x / Plant J. / Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis thaliana. by Clough S. (1999)
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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 4:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 28, 2023, 1:12 p.m.)
Indexed	4 weeks ago (Aug. 5, 2025, 8:25 a.m.)
Issued	26 years, 1 month ago (Aug. 1, 1999)
Published	26 years, 1 month ago (Aug. 1, 1999)
Published Online	23 years, 7 months ago (Jan. 5, 2002)
Published Print	26 years, 1 month ago (Aug. 1, 1999)

Funders 0

None

BibTeX

@article{Ye_1999, title={Arabidopsis ovule is the target for Agrobacterium in planta vacuum infiltration transformation}, volume={19}, ISSN={1365-313X}, url={http://dx.doi.org/10.1046/j.1365-313x.1999.00520.x}, DOI={10.1046/j.1365-313x.1999.00520.x}, number={3}, journal={The Plant Journal}, publisher={Wiley}, author={Ye, Guang‐Ning and Stone, Deborah and Pang, Sheng‐Zhi and Creely, Wendi and Gonzalez, Kathleen and Hinchee, Maud}, year={1999}, month=aug, pages={249–257} }

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