DOI: 10.1046/j.1365-2958.2003.03826.x. Macroautophagy is dispensable for intracellular replication of <i>Legionella pneumophila</i> in <i>Dictyostelium discoideum</i>

Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum

10.1046/j.1365-2958.2003.03826.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe Gram‐negative bacterium Legionella pneumophila is a facultative intracellular pathogen of free‐living amoebae and mammalian phagocytes. L. pneumophila is engulfed in phagosomes that initially avoid fusion with lysosomes. The phagosome associates with endoplasmic reticulum (ER) and mitochondria and eventually resembles ER. The morphological similarity of the replication vacuole to autophagosomes, and enhanced bacterial replication in response to macroautophagy‐inducing starvation, led to the hypothesis that L. pneumophila infection requires macroautophagy. As L. pneumophila replicates in Dictyostelium discoideum, and macroautophagy genes have been identified and mutated in D. discoideum, we have taken a genetic and cell biological approach to evaluate the relationship between host macroautophagy and intracellular replication of L. pneumophila. Mutation of the apg1, apg5, apg6, apg7 and apg8 genes produced typical macroautophagy defects, including reduced bulk protein degradation and cell viability during starvation. We show that L. pneumophila replicates normally in D. discoideum macroautophagy mutants and produces replication vacuoles that are morphologically indistinguishable from those in wild‐type D. discoideum. Furthermore, a green fluorescent protein (GFP)‐tagged marker of autophagosomes, Apg8, does not systematically co‐localize with DsRed‐labelled L. pneumophila. We conclude that macroautophagy is dispensable for L. pneumophila intracellular replication in D. discoideum.

Bibliography

Otto, G. P., Wu, M. Y., Clarke, M., Lu, H., Anderson, O. R., Hilbi, H., Shuman, H. A., & Kessin, R. H. (2003). Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum. Molecular Microbiology, 51(1), 63â72. Portico.

Authors 8

Grant P. Otto (first)
Mary Y. Wu (additional)
Margaret Clarke (additional)
Hao Lu (additional)
O. Roger Anderson (additional)
Hubert Hilbi (additional)
Howard A. Shuman (additional)
Richard H. Kessin (additional)

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 27, 2004, 5:55 p.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 5:33 a.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:54 p.m.)
Issued	21 years, 9 months ago (Nov. 11, 2003)
Published	21 years, 9 months ago (Nov. 11, 2003)
Published Online	21 years, 9 months ago (Nov. 11, 2003)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Otto_2003, title={Macroautophagy is dispensable for intracellular replication of Legionella pneumophila in Dictyostelium discoideum}, volume={51}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2003.03826.x}, DOI={10.1046/j.1365-2958.2003.03826.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Otto, Grant P. and Wu, Mary Y. and Clarke, Margaret and Lu, Hao and Anderson, O. Roger and Hilbi, Hubert and Shuman, Howard A. and Kessin, Richard H.}, year={2003}, month=nov, pages={63–72} }

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