DOI: 10.1046/j.1365-2958.2003.03642.x. A subset of bacterial inner membrane proteins integrated by the twin‐arginine translocase

A subset of bacterial inner membrane proteins integrated by the twin‐arginine translocase

10.1046/j.1365-2958.2003.03642.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryA group of bacterial exported proteins are synthesized with N‐terminal signal peptides containing a SRRxFLK ‘twin‐arginine’ amino acid motif. Proteins bearing twin‐arginine signal peptides are targeted post‐translationally to the twin‐arginine translocation (Tat) system which transports folded substrates across the inner membrane. In Escherichia coli, most integral inner membrane proteins are assembled by a co‐translational process directed by SRP/FtsY, the SecYEG translocase, and YidC. In this work we define a novel class of integral membrane proteins assembled by a Tat‐dependent mechanism. We show that at least five E. coli Tat substrate proteins contain hydrophobic C‐terminal transmembrane helices (or ‘C‐tails’). Fusions between the identified transmembrane C‐tails and the exclusively Tat‐dependent reporter proteins TorA and SufI render the resultant chimeras membrane‐bound. Export‐linked signal peptide processing and membrane integration of the chimeras is shown to be both Tat‐dependent and YidC‐independent. It is proposed that the mechanism of membrane integration of proteins by the Tat system is fundamentally distinct from that employed for other bacterial inner membrane proteins.

Bibliography

Hatzixanthis, K., Palmer, T., & Sargent, F. (2003). A subset of bacterial inner membrane proteins integrated by the twinâarginine translocase. Molecular Microbiology, 49(5), 1377â1390. Portico.

Authors 3

Kostas Hatzixanthis (first)
Tracy Palmer (additional)
Frank Sargent (additional)

References 60 Referenced 113

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Dates

Type	When
Created	22 years ago (Aug. 27, 2003, 5:57 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 12:46 p.m.)
Indexed	6 hours, 41 minutes ago (Sept. 3, 2025, 6:01 a.m.)
Issued	22 years ago (Aug. 27, 2003)
Published	22 years ago (Aug. 27, 2003)
Published Online	22 years ago (Aug. 27, 2003)
Published Print	22 years ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Hatzixanthis_2003, title={A subset of bacterial inner membrane proteins integrated by the twin‐arginine translocase}, volume={49}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2003.03642.x}, DOI={10.1046/j.1365-2958.2003.03642.x}, number={5}, journal={Molecular Microbiology}, publisher={Wiley}, author={Hatzixanthis, Kostas and Palmer, Tracy and Sargent, Frank}, year={2003}, month=aug, pages={1377–1390} }

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    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2003,
        8,
        27
      ]
    ]
  }
}