DOI: 10.1046/j.1365-2958.2003.03567.x. Identification of additional TnrA‐regulated genes of <i>Bacillus subtilis</i> associated with a TnrA box

Identification of additional TnrA‐regulated genes of Bacillus subtilis associated with a TnrA box

10.1046/j.1365-2958.2003.03567.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Bacillus subtilis TnrA is a global regulator that responds to the availability of nitrogen sources and both activates and represses many genes during nitrogen‐limited growth. In order to obtain a holistic view of the gene regulation depending on TnrA, we performed a genome‐wide screening for TnrA‐regulated genes associated with a TnrA box. A combination of DNA microarray hybridization and a genome‐wide search for TnrA boxes allowed us to find 36 TnrA‐regulated transcription units associated with a putative TnrA box. Gel retardation assaying, using probes carrying at least one putative TnrA box and the deletion derivatives of each box, indicated that 17 out of 36 transcription units were likely TnrA targets associated with the TnrA boxes, two of which (nasA and nasBCDEF) possessed a common TnrA box. The sequences of these TnrA boxes contained a consensus one, TGTNANAWWWTMTNACA. The TnrA targets detected in this study were nrgAB, pucJKLM, glnQHMP, nasDEF, oppABCDF, nasA, nasBCDEF and ywrD for positive regulation, and gltAB, pel, ywdIJK, yycCB, yttA, yxkC, ywlFG, yodF and alsT for negative regulation, nrgAB and gltAB being well‐studied TnrA targets. It was unexpected that the negatively regulated TnrA targets were as many as the positively regulated targets. The physiological role of the TnrA regulon is discussed.

Bibliography

Yoshida, K., Yamaguchi, H., Kinehara, M., Ohki, Y., Nakaura, Y., & Fujita, Y. (2003). Identification of additional TnrAâregulated genes of Bacillus subtilis associated with a TnrA box. Molecular Microbiology, 49(1), 157â165. Portico.

Authors 6

Ken‐ichi Yoshida (first)
Hirotake Yamaguchi (additional)
Masaki Kinehara (additional)
Yo‐hei Ohki (additional)
Yoshiko Nakaura (additional)
Yasutaro Fujita (additional)

References 30 Referenced 90

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Dates

Type	When
Created	22 years, 2 months ago (June 19, 2003, 6:08 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 5:56 p.m.)
Indexed	4 months, 1 week ago (April 21, 2025, 6:43 p.m.)
Issued	22 years, 2 months ago (June 3, 2003)
Published	22 years, 2 months ago (June 3, 2003)
Published Online	22 years, 2 months ago (June 3, 2003)
Published Print	22 years, 2 months ago (July 1, 2003)

Funders 0

None

BibTeX

@article{Yoshida_2003, title={Identification of additional TnrA‐regulated genes of Bacillus subtilis associated with a TnrA box}, volume={49}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2003.03567.x}, DOI={10.1046/j.1365-2958.2003.03567.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Yoshida, Ken‐ichi and Yamaguchi, Hirotake and Kinehara, Masaki and Ohki, Yo‐hei and Nakaura, Yoshiko and Fujita, Yasutaro}, year={2003}, month=jun, pages={157–165} }

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