DOI: 10.1046/j.1365-2958.2003.03549.x. A novel sheathed surface organelle of the <i>Helicobacter pylori cag</i> type IV secretion system

A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system

10.1046/j.1365-2958.2003.03549.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryType I strains of Helicobacter pylori (Hp) use a type IV secretion system (T4SS), encoded by the cag pathogenicity island (cag‐PAI), to deliver the bacterial protein CagA into eukaryotic cells and to induce interleukin‐8 secretion. Translocated CagA is activated by tyrosine phosphorylation involving Src‐family kinases. The mechanism and structural basis for type IV protein secretion is not well understood. We describe here, by confocal laser scanning microscopy and field emission scanning electron microscopy, a novel filamentous surface organelle which is part of the Hp T4SS. The organelle is often located at one bacterial pole but can be induced by cell contact also along the lateral side of the bacteria. It consists of a rigid needle, covered focally or completely by HP0527 (Cag7 or CagY), a VirB10‐homologous protein. HP0527 is also clustered in the outer membrane. The VirB7‐homologous protein HP0532 is found at the base of this organelle. These observations demonstrate for the first time by microscopic techniques a complex T4SS‐associated, sheathed surface organelle reminiscent to the needle structures of bacterial type III secretion systems.

Bibliography

Rohde, M., PÃ¼ls, J., Buhrdorf, R., Fischer, W., & Haas, R. (2003). A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system. Molecular Microbiology, 49(1), 219â234. Portico.

Authors 5

Manfred Rohde (first)
Jürgen Püls (additional)
Renate Buhrdorf (additional)
Wolfgang Fischer (additional)
Rainer Haas (additional)

References 45 Referenced 210

10.1038/16495
10.1084/jem.191.4.593
10.1046/j.1462-5822.2000.00043.x
10.1046/j.1365-2958.2001.02649.x
10.1046/j.1365-2958.2001.02200.x
10.1016/S0966-842X(00)01792-3
10.1046/j.1365-2958.2001.02443.x
10.1126/science.284.5418.1328
10.1136/jcp.48.10.967
10.1046/j.1365-2958.1998.01046.x
10.1046/j.1365-2958.2001.02714.x
10.1128/IAI.69.11.6769-6775.2001
10.1078/1438-4221-00199
10.1074/jbc.M007617200
10.1126/science.284.5418.1322
10.1016/0092-8674(94)90510-X
10.1126/science.1067147
10.1073/pnas.222375399
10.1073/pnas.071065798
10.1016/0003-2697(81)90473-5
{'key': 'e_1_2_6_22_1', 'volume-title': 'IARC Monographs on the Evaluation of Carcinogenic Risks to Humans.', 'author': 'International Agency for Research on Cancer', 'year': '1994'} / IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. by International Agency for Research on Cancer (1994)
10.1046/j.1365-2958.2001.02455.x
10.1128/IAI.68.8.4598-4603.2000
10.1046/j.1364-3703.2002.00104.x
10.1093/emboj/17.8.2166
10.1126/science.280.5363.602
10.1046/j.1365-2958.2002.02829.x
10.1046/j.1365-2958.2000.01876.x
10.1128/IAI.70.4.2108-2120.2002
10.1073/pnas.96.12.7011
10.1016/S1097-2765(02)00681-0
10.1111/j.1432-1033.1994.1089b.x
10.1046/j.1440-169x.1999.00406.x
10.1073/pnas.96.14.8173
10.1126/science.287.5457.1497
10.1046/j.1462-5822.2001.00088.x
10.1016/0378-1119(93)90354-6
10.1046/j.1365-2958.2002.02780.x
10.1128/IAI.65.9.3622-3630.1997
10.1073/pnas.94.7.3459
10.1073/pnas.96.25.14559
10.1016/S0022-5320(69)90033-1
10.1073/pnas.97.3.1263
10.1046/j.1365-2958.2002.02781.x
10.1038/41483

Dates

Type	When
Created	22 years, 2 months ago (June 19, 2003, 6:08 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 5:55 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:34 a.m.)
Issued	22 years, 3 months ago (May 30, 2003)
Published	22 years, 3 months ago (May 30, 2003)
Published Online	22 years, 3 months ago (May 30, 2003)
Published Print	22 years, 2 months ago (July 1, 2003)

Funders 0

None

BibTeX

@article{Rohde_2003, title={A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system}, volume={49}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2003.03549.x}, DOI={10.1046/j.1365-2958.2003.03549.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Rohde, Manfred and Püls, Jürgen and Buhrdorf, Renate and Fischer, Wolfgang and Haas, Rainer}, year={2003}, month=may, pages={219–234} }

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