DOI: 10.1046/j.1365-2958.2002.03235.x. Subcellular localization of the <i>Bacillus subtilis</i> structural maintenance of chromosomes (SMC) protein

Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein

10.1046/j.1365-2958.2002.03235.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe Bacillus subtilis structural maintenance of chromosomes (SMC) protein is a member of a large family of proteins involved in chromosome organization. We found that SMC is a moderately abundant protein (∼1000 dimers per cell). In vivo cross‐linking and immunoprecipitation assays revealed that SMC binds to many regions on the chromosome. Visualization of SMC in live cells using a fusion to the green fluorescent protein (GFP) and in fixed cells using immunofluorescence microscopy indicated that a portion of SMC localizes as discrete foci in positions similar to that of the DNA replication machinery (replisome). When visualized simultaneously, SMC and the replisome were often in similar regions of the cell but did not always co‐localize. Persistence of SMC foci did not depend on ongoing replication, but did depend on ScpA and ScpB, two proteins thought to interact with SMC. Our results indicate that SMC is bound to many sites on the chromosome and a concentration of SMC is localized near replication forks, perhaps there to bind and organize newly replicated DNA.

Bibliography

Lindow, J. C., Kuwano, M., Moriya, S., & Grossman, A. D. (2002). Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein. Molecular Microbiology, 46(4), 997â1009. Portico.

Authors 4

Janet C. Lindow (first)
Masayoshi Kuwano (additional)
Shigeki Moriya (additional)
Alan D. Grossman (additional)

References 67 Referenced 71

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:46 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 9:23 a.m.)
Indexed	3 weeks, 4 days ago (Aug. 5, 2025, 9:11 a.m.)
Issued	22 years, 9 months ago (Nov. 1, 2002)
Published	22 years, 9 months ago (Nov. 1, 2002)
Published Online	22 years, 9 months ago (Nov. 6, 2002)
Published Print	22 years, 9 months ago (Nov. 1, 2002)

Funders 0

None

BibTeX

@article{Lindow_2002, title={Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein}, volume={46}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.03235.x}, DOI={10.1046/j.1365-2958.2002.03235.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Lindow, Janet C. and Kuwano, Masayoshi and Moriya, Shigeki and Grossman, Alan D.}, year={2002}, month=nov, pages={997–1009} }

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  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2002,
          11
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.2002.03235.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.2002.03235.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2002,
        11
      ]
    ]
  }
}