DOI: 10.1046/j.1365-2958.2002.02836.x. Regulation of transcription by the activity of the <i>Shigella flexneri</i> type III secretion apparatus

Regulation of transcription by the activity of the Shigella flexneri type III secretion apparatus

10.1046/j.1365-2958.2002.02836.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe virulence plasmid‐encoded type III secretion system of Shigella flexneri consists of the Mxi–Spa secretion apparatus, secreted proteins IpaA–D and IpgD involved in entry of bacteria into epithelial cells, cytoplasmic chaperones IpgC and IpgE and 15 other secreted proteins of unknown function, including VirA and members of the IpaH family. The activity of the Mxi–Spa apparatus is regulated by external signals, and transcription of virA and ipaH genes is specifically induced in conditions of active secretion. We present genetic evidence that regulation of these genes involves both MxiE, the transcriptional activator of the AraC family encoded by the mxi operon, and IpgC, the chaperone for IpaB and IpaC. We also show that together MxiE and IpgC are sufficient to activate virA and ipaH9.8 promoters in Escherichia coli. In S. flexneri, increasing the expression of IpgC led to a concomitant increase in IpaH production in conditions of non‐secretion. This suggests that the activity of secretion is sensed by the presence of free IpgC, which acts as a coactivator to allow MxiE to activate transcription at its target promoters.

Bibliography

Mavris, M., Page, A., Tournebize, R., Demers, B., Sansonetti, P., & Parsot, C. (2002). Regulation of transcription by the activity of the Shigella flexneri type III secretion apparatus. Molecular Microbiology, 43(6), 1543â1553. Portico.

Authors 6

Maria Mavris (first)
Anne‐Laure Page (additional)
Régis Tournebize (additional)
Brigitte Demers (additional)
Philippe Sansonetti (additional)
Claude Parsot (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:43 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 11:31 p.m.)
Indexed	1 month ago (July 23, 2025, 8:24 a.m.)
Issued	23 years, 5 months ago (March 1, 2002)
Published	23 years, 5 months ago (March 1, 2002)
Published Online	23 years, 4 months ago (April 25, 2002)
Published Print	23 years, 5 months ago (March 1, 2002)

Funders 0

None

BibTeX

@article{Mavris_2002, title={Regulation of transcription by the activity of the Shigella flexneri type III secretion apparatus}, volume={43}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.02836.x}, DOI={10.1046/j.1365-2958.2002.02836.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Mavris, Maria and Page, Anne‐Laure and Tournebize, Régis and Demers, Brigitte and Sansonetti, Philippe and Parsot, Claude}, year={2002}, month=mar, pages={1543–1553} }

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