DOI: 10.1046/j.1365-2958.2002.02824.x. Rpa12p, a conserved RNA polymerase I subunit with two functional domains

Rpa12p, a conserved RNA polymerase I subunit with two functional domains

10.1046/j.1365-2958.2002.02824.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryRpa12p is a subunit of RNA polymerase I formed of two zinc‐binding domains. The N‐terminal zinc region (positions 1–60) is poorly conserved from yeast to man. The C‐terminal domain contains an invariant Q.RSADE..T.F motif shared with the TFIIS elongation factor of RNA polymerase II and its archaeal counterpart. Deletions removing the N‐terminal domain fail to grow at 34°C, are sensitive to nucleotide‐depleting drugs and become lethal in rpa14‐Δ mutants lacking the non‐essential RNA polymerase I subunit Rpa14p. They also strongly alter the immunofluorescent properties of RNA polymerase I in the nucleolus. Finally, they prevent the binding of Rpa12p to immunopurified polymerase I and impair a specific two‐hybrid interaction with the second largest subunit. In all these respects, N‐terminal deletions behave like full deletions. In contrast, C‐terminal deletions retaining only the first N‐terminal 60 amino acids are indistinguishable from wild type. Thus, the N‐terminal zinc domain of Rpa12p determines its anchoring to RNA polymerase I and is the only critical part of that subunit in vivo.

Bibliography

Mullem, V. V., Landrieux, E., Vandenhaute, J., & Thuriaux, P. (2002). Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Molecular Microbiology, 43(5), 1105â1113. Portico.

Authors 4

Vincent Van Mullem (first)
Emilie Landrieux (additional)
Jean Vandenhaute (additional)
Pierre Thuriaux (additional)

References 40 Referenced 49

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 11:43 a.m.)
Indexed	1 month, 3 weeks ago (July 8, 2025, 6:43 a.m.)
Issued	23 years, 6 months ago (March 1, 2002)
Published	23 years, 6 months ago (March 1, 2002)
Published Online	23 years, 5 months ago (March 22, 2002)
Published Print	23 years, 6 months ago (March 1, 2002)

Funders 0

None

BibTeX

@article{Mullem_2002, title={Rpa12p, a conserved RNA polymerase I subunit with two functional domains}, volume={43}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.02824.x}, DOI={10.1046/j.1365-2958.2002.02824.x}, number={5}, journal={Molecular Microbiology}, publisher={Wiley}, author={Mullem, Vincent Van and Landrieux, Emilie and Vandenhaute, Jean and Thuriaux, Pierre}, year={2002}, month=mar, pages={1105–1113} }

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