DOI: 10.1046/j.1365-2958.2002.02798.x. Inactivation of the <i>srtA</i> gene in <i>Listeria monocytogenes</i> inhibits anchoring of surface proteins and affects virulence

Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence

10.1046/j.1365-2958.2002.02798.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryDuring infection of their hosts, Gram‐positive bac‐teria express surface proteins that serve multiple biological functions. Surface proteins harbouring a C‐terminal sorting signal with an LPXTG motif are covalently linked to the cell wall peptidoglycan by a transamidase named sortase. Two genes encoding putative sortases, termed srtA and srtB, were identified in the genome of the intracellular pathogenic bacterium Listeria monocytogenes. Inactivation of srtA abolishes anchoring of the invasion protein InlA to the bacterial surface. It also prevents the proper sorting of several other peptidoglycan‐associated LPXTG proteins. Three were identified by a mass spectrometry approach. The ΔsrtA mutant strain is defective in entering epithelial cells, similar to a ΔinlA mutant. In contrast to a ΔinlA mutant, the ΔsrtA mutant is impaired for colonization of the liver and spleen after oral inoculation in mice. Thus, L. monocytogenes srtA is required for the cell wall anchoring of InlA and, presumably, for the anchoring of other LPXTG‐containing proteins that are involved in listerial infections.

Bibliography

Bierne, H., Mazmanian, S. K., Trost, M., Pucciarelli, M. G., Liu, G., Dehoux, P., JÃ¤nsch, L., Portillo, F. G., Schneewind, O., & Cossart, P. (2002). Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence. Molecular Microbiology, 43(4), 869â881. Portico.

Authors 11

Hélène Bierne (first)
Sarkis K. Mazmanian (additional)
Matthias Trost (additional)
M. Graciela Pucciarelli (additional)
Gwen Liu (additional)
Pierre Dehoux (additional)
(additional)
Lothar Jänsch (additional)
Francisco Garcia‐del Portillo (additional)
Olaf Schneewind (additional)
Pascale Cossart (additional)

References 44 Referenced 190

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 5:56 a.m.)
Indexed	2 days, 20 hours ago (Sept. 3, 2025, 7:06 a.m.)
Issued	23 years, 7 months ago (Feb. 1, 2002)
Published	23 years, 7 months ago (Feb. 1, 2002)
Published Online	23 years, 5 months ago (March 27, 2002)
Published Print	23 years, 7 months ago (Feb. 1, 2002)

Funders 0

None

BibTeX

@article{Bierne_2002, title={Inactivation of the srtA gene in Listeria monocytogenes inhibits anchoring of surface proteins and affects virulence}, volume={43}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.02798.x}, DOI={10.1046/j.1365-2958.2002.02798.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Bierne, Hélène and Mazmanian, Sarkis K. and Trost, Matthias and Pucciarelli, M. Graciela and Liu, Gwen and Dehoux, Pierre and Jänsch, Lothar and Portillo, Francisco Garcia‐del and Schneewind, Olaf and Cossart, Pascale}, year={2002}, month=feb, pages={869–881} }

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        2
      ]
    ]
  },
  "references-count": 44,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2002,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.2002.02798.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.2002.02798.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2002,
        2
      ]
    ]
  }
}