DOI: 10.1046/j.1365-2958.2002.02793.x. A putative three‐dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in <i>Erwinia carotovora</i>

A putative three‐dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora

10.1046/j.1365-2958.2002.02793.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryIntramolecular information specifying protein secretion through the type II (GSP) pathway of Gram‐negative bacteria was investigated. Two regions of the polygalacturonase (PehA) of Erwinia carotovora containing residues proposed to be included in a targeting motif were located, one close to the C‐terminus between residues 342 and 369 and another between residues 84 and 135 in the large central loops. The regions were required together to promote secretion. Further residues in the middle of the protein were required for proper positioning of the regions, suggesting that they were both involved in interaction with the GSP. To our knowledge, this is the first time that a possible three‐dimensional targeting motif has been defined. At least one of the motifs comprises a cluster on the surface of the protein. The two motifs are structurally dissimilar, suggesting that there are two distinct recognition regions in the GSP apparatus. Finally, we propose that the targeting motifs are of a complex conformational nature with some variability accommodated, as illustrated by the observation that many mutations exhibited no clear phenotype individually but, in combination, severely compromised secretion.

Bibliography

PalomÃ¤ki, T., Pickersgill, R., Riekki, R., Romantschuk, M., & Saarilahti, H. T. (2002). A putative threeâdimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora. Molecular Microbiology, 43(3), 585â596. Portico.

Authors 5

Tiina Palomäki (first)
Richard Pickersgill (additional)
Ruusu Riekki (additional)
Martin Romantschuk (additional)
Hannu T. Saarilahti (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 5:51 p.m.)
Indexed	1 year, 10 months ago (Oct. 27, 2023, 2:24 p.m.)
Issued	23 years, 6 months ago (Feb. 1, 2002)
Published	23 years, 6 months ago (Feb. 1, 2002)
Published Online	23 years, 6 months ago (Feb. 28, 2002)
Published Print	23 years, 6 months ago (Feb. 1, 2002)

Funders 0

None

BibTeX

@article{Palom_ki_2002, title={A putative three‐dimensional targeting motif of polygalacturonase (PehA), a protein secreted through the type II (GSP) pathway in Erwinia carotovora}, volume={43}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.02793.x}, DOI={10.1046/j.1365-2958.2002.02793.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Palomäki, Tiina and Pickersgill, Richard and Riekki, Ruusu and Romantschuk, Martin and Saarilahti, Hannu T.}, year={2002}, month=feb, pages={585–596} }

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