DOI: 10.1046/j.1365-2958.2002.02738.x. Molecular characterization of type III secretion signals via analysis of synthetic N‐terminal amino acid sequences

Molecular characterization of type III secretion signals via analysis of synthetic N‐terminal amino acid sequences

10.1046/j.1365-2958.2002.02738.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Yersinia species utilize a type III secretion system to inject toxins, called Yops (Yersinia outer proteins), into eukaryotic cells. The N‐termini of the Yops serve as type III secretion signals, but they do not share a consensus sequence. To simplify the analysis of type III secretion signals, we replaced amino acids 2–8 of the secreted protein YopE with all permutations (27 or 128) of synthetic serine/isoleucine sequences. The results demonstrate that amphipathic N‐terminal sequences, containing four or five serine residues, have a much greater probability than hydrophobic or hydrophilic sequences to target YopE for secretion. Multiple linear regression analysis of the synthetic sequences was used to obtain a model for N‐terminal secretion signals. The model accurately classifies the N‐terminal sequences of native type III substrates as efficient secretion signals.

Authors 4

Scott A. Lloyd (first)
Michael Sjöström (additional)
Sara Andersson (additional)
Hans Wolf‐Watz (additional)

References 33 Referenced 87

10.1126/science.278.5340.1140 / Science / A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica [see comments]. by Anderson D.M. (1997)
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10.1016/S0022-2836(83)80284-8
10.1128/MMBR.62.2.379-433.1998 / Microbiol Mol Biol Rev / Type III protein secretion systems in bacterial pathogens of animals and plants. by Hueck C.J. (1998)
10.1128/jb.163.1.267-274.1985 / J Bacteriol / Evidence for specificity at an early step in protein export in Escherichia coli. by Kumamoto C.A. (1985)
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10.1046/j.1365-2958.2001.02271.x
10.1128/iai.58.9.2840-2849.1990 / Infect Immun / Secretion of Yop proteins by Yersiniae. by Michiels T. (1990)
10.1128/jb.178.5.1310-1319.1996
10.1128/iai.43.1.108-114.1984 / Infect Immun / Characterization of common virulence plasmids in Yersinia species and their role in the expression of outer membrane proteins. by Portnoy D.A. (1985)
10.1111/j.1742-4658.2006.05480.x
10.1128/iai.56.8.2139-2143.1988 / Infect Immun / Inhibition of phagocytosis in Yersinia pseudotuberculosis: a virulence plasmid‐encoded ability involving the Yop2b protein. by Rosqvist R. (1988)
10.1128/iai.59.12.4562-4569.1991 / Infect Immun / Intracellular targeting of the Yersinia YopE cytotoxin in mammalian cells induces actin microfilament disruption. by Rosqvist R. (1991)
10.1128/jb.178.24.7227-7233.1996
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10.1016/S0092-8674(00)81839-5
10.1046/j.1365-2958.2000.01898.x
10.1038/283224a0

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:41 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 8:45 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 13, 2024, 12:43 p.m.)
Issued	23 years, 8 months ago (Jan. 1, 2002)
Published	23 years, 8 months ago (Jan. 1, 2002)
Published Online	23 years, 6 months ago (Feb. 28, 2002)
Published Print	23 years, 8 months ago (Jan. 1, 2002)

Funders 0

None

BibTeX

@article{Lloyd_2002, title={Molecular characterization of type III secretion signals via analysis of synthetic N‐terminal amino acid sequences}, volume={43}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2002.02738.x}, DOI={10.1046/j.1365-2958.2002.02738.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Lloyd, Scott A. and Sjöström, Michael and Andersson, Sara and Wolf‐Watz, Hans}, year={2002}, month=jan, pages={51–59} }

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