DOI: 10.1046/j.1365-2958.2001.02690.x. Overexpression of the alternative oxidase restores senescence and fertility in a long‐lived respiration‐deficient mutant of <i>Podospora anserina</i>

Overexpression of the alternative oxidase restores senescence and fertility in a long‐lived respiration‐deficient mutant of Podospora anserina

10.1046/j.1365-2958.2001.02690.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Several lines of evidence have implicated reactive oxygen species (ROS) in the pathogenesis of various degenerative diseases and in organismal ageing. Furthermore, it has been shown recently that the alternative pathway respiration present in plants lowers ROS mitochondrial production. An alternative oxidase (AOXp) also occurs in the filamentous fungus Podospora anserina. We show here that overexpression of this oxidase does not decrease ROS production and has no effect on longevity, mitochondrial stability or ageing in this fungus. In the same way, inactivation of the gene has no effect on these parameters. In contrast, overexpression of the alternative oxidase in the long‐lived cox5::BLE mutant, deficient in cytochrome c oxidase, considerably increases ROS production of the mutant. It rescues slow growth rate and female sterility, indicating an improved energy level. This overexpression also restores senescence and mitochondrial DNA instability, demonstrating that these parameters are controlled by the energy level and not by the expression level of the alternative oxidase. We also suggest that expression of this oxidase in organisms naturally devoid of it could rescue respiratory defects resulting from cytochrome pathway dysfunctions.

Bibliography

Lorin, S., Dufour, E., Boulay, J., Begel, O., Marsy, S., & SainsardâChanet, A. (2001). Overexpression of the alternative oxidase restores senescence and fertility in a longâlived respirationâdeficient mutant of Podospora anserina. Molecular Microbiology, 42(5), 1259â1267. Portico.

Authors 6

Séverine Lorin (first)
Eric Dufour (additional)
Jocelyne Boulay (additional)
Odile Begel (additional)
Sophie Marsy (additional)
Annie Sainsard‐Chanet (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:41 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 12:32 a.m.)
Indexed	2 months, 3 weeks ago (June 16, 2025, 4:24 p.m.)
Issued	23 years, 9 months ago (Dec. 1, 2001)
Published	23 years, 9 months ago (Dec. 1, 2001)
Published Online	23 years, 7 months ago (Jan. 13, 2002)
Published Print	23 years, 9 months ago (Dec. 1, 2001)

Funders 0

None

BibTeX

@article{Lorin_2001, title={Overexpression of the alternative oxidase restores senescence and fertility in a long‐lived respiration‐deficient mutant of Podospora anserina}, volume={42}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2001.02690.x}, DOI={10.1046/j.1365-2958.2001.02690.x}, number={5}, journal={Molecular Microbiology}, publisher={Wiley}, author={Lorin, Séverine and Dufour, Eric and Boulay, Jocelyne and Begel, Odile and Marsy, Sophie and Sainsard‐Chanet, Annie}, year={2001}, month=dec, pages={1259–1267} }

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