DOI: 10.1046/j.1365-2958.2001.02495.x. The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2

The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2

10.1046/j.1365-2958.2001.02495.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Structural genes of phospholipid biosynthesis in the yeastSaccharomyces cerevisiaeare transcriptionally co‐regulated by ICRE (inositol/choline‐responsive element) promoter motifs. Gene activation by an ICRE is mediated by binding of the Ino2/Ino4 transcription factor, whereas repression in the presence of high concentrations of inositol and choline (IC) requires an intact Opi1 repressor. However, the mechanism of specific repression and the functional interplay among these regulators remained unclear from previous work. Usingin vivoas well asin vitrointeraction assays, we show binding of the pleiotropic repressor Sin3 to the pathway‐specific regulator Opi1. The paired amphipathic helix 1 (PAH1) within Sin3 and OSID (Opi1–Sin3 interaction domain) in the N‐terminus of Opi1 were mapped as contact sites. The regulatory significance of the Opi1–Sin3 interaction was shown by the obvious deregulation of an ICRE‐dependent reporter gene in asin3mutant. Opi1 also interacts with a newly identified functional domain of the transcriptional activator Ino2 (RID, repressor interaction domain). These results define the molecular composition of the transcription complex mediating control of ICRE‐dependent genes and allow a hypothesis on the flow of regulatory information in response to phospholipid precursors.

Bibliography

Wagner, C., Dietz, M., Wittmann, J., Albrecht, A., & SchÃ¼ller, H. (2001). The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2. Molecular Microbiology, 41(1), 155â166. Portico.

Authors 5

Christian Wagner (first)
Martin Dietz (additional)
Jürgen Wittmann (additional)
Antje Albrecht (additional)
Hans‐Joachim Schüller (additional)

References 58 Referenced 93

10.1038/387049a0
10.1016/0092-8674(95)90355-0
10.1128/MCB.16.10.5772
10.1074/jbc.270.42.25087
10.1093/nar/20.6.1411
10.1093/genetics/154.2.573
10.1101/gad.10.10.1247
10.1074/jbc.274.46.32750
10.1093/nar/28.16.3160
10.1007/BF00331845
10.1016/S0021-9258(18)82077-4
10.1038/387043a0
10.1016/S0079-6603(08)60826-0
10.1016/s0014-5793(97)01085-5
10.1093/oxfordjournals.jbchem.a124287
10.1016/S0021-9258(19)39624-3 / J Biol Chem / The Saccharomyces cerevisiae INO4 gene encodes a small, highly basic protein required for derepression of phospholipid biosynthesis enzymes by Hoshizaki D.K. (1990)
10.1093/genetics/136.2.475 / Genetics / A pleiotropic phospholipid biosynthetic regulatory mutation in Saccharomyces cerevisiae is allelic to sin3 (sdi1, ume4, rpd1) by Hudak K.A. (1994)
10.1093/genetics/144.4.1425
10.1016/S0092-8674(00)80217-2
10.1128/MCB.18.9.5121
10.1007/s004380050581
10.1128/MCB.17.8.4852
10.1016/0092-8674(92)90146-4
10.1046/j.1365-2958.1997.5611937.x
10.1016/S0092-8674(00)80215-9
10.1093/oso/9780198502487.001.0001 / Helix–Loop–Helix Transcription Factors by Littlewood T.D. (1998)
10.1093/nar/19.14.3987
10.1093/nar/19.7.1687
10.1128/jb.175.13.4235-4238.1993
10.1093/nar/22.25.5767
10.1016/0092-8674(87)90236-4
10.1093/nar/20.12.3253
10.1073/pnas.93.25.14503
10.1002/(sici)1521-1878(199810)20:10<808::aid-bies6>3.3.co;2-l
10.1016/0092-8674(95)90356-9
10.1002/j.1460-2075.1992.tb05033.x
10.1093/nar/20.22.5955
10.1016/0014-5793(95)00818-t
10.1093/nar/23.2.230
10.1007/s002940050231
10.1093/nar/23.11.1964
10.1016/s0968-0004(00)01592-9
10.1016/0092-8674(87)90235-2
10.1101/gad.8.7.796
10.1093/genetics/144.4.1343
10.1128/MCB.11.12.6306
10.1073/pnas.92.6.2370
10.1038/35044127
10.1002/yea.320101310
10.1002/(sici)1097-0061(199907)15:10a<843::aid-yea424>3.3.co;2-d
10.1128/MCB.13.3.1805
10.1128/MCB.10.11.5927
10.1101/gad.829100
10.1016/S0021-9258(17)35253-5 / J Biol Chem / The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper by White M.J. (1991)
10.1128/MCB.10.12.6500
10.1007/BF00587597
10.1016/S0092-8674(00)80216-0
10.1016/S1097-2765(00)80102-1

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:38 a.m.)
Deposited	1 year, 7 months ago (Jan. 9, 2024, 3:42 a.m.)
Indexed	1 month, 4 weeks ago (July 2, 2025, 3:07 p.m.)
Issued	24 years, 2 months ago (July 1, 2001)
Published	24 years, 2 months ago (July 1, 2001)
Published Online	23 years, 8 months ago (Dec. 21, 2001)
Published Print	24 years, 2 months ago (July 1, 2001)

Funders 0

None

BibTeX

@article{Wagner_2001, title={The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2}, volume={41}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2001.02495.x}, DOI={10.1046/j.1365-2958.2001.02495.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Wagner, Christian and Dietz, Martin and Wittmann, Jürgen and Albrecht, Antje and Schüller, Hans‐Joachim}, year={2001}, month=jul, pages={155–166} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T19:07:58Z",
    "timestamp": 1751483278425
  },
  "reference-count": 58,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            12,
            21
          ]
        ],
        "date-time": "2001-12-21T00:00:00Z",
        "timestamp": 1008892800000
      },
      "content-version": "vor",
      "delay-in-days": 173,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        7
      ]
    ]
  },
  "abstract": "<jats:p>Structural genes of phospholipid biosynthesis in the yeast<jats:italic>Saccharomyces cerevisiae</jats:italic>are transcriptionally co\u2010regulated by ICRE (inositol/choline\u2010responsive element) promoter motifs. Gene activation by an ICRE is mediated by binding of the Ino2/Ino4 transcription factor, whereas repression in the presence of high concentrations of inositol and choline (IC) requires an intact Opi1 repressor. However, the mechanism of specific repression and the functional interplay among these regulators remained unclear from previous work. Using<jats:italic>in vivo</jats:italic>as well as<jats:italic>in vitro</jats:italic>interaction assays, we show binding of the pleiotropic repressor Sin3 to the pathway\u2010specific regulator Opi1. The paired amphipathic helix 1 (PAH1) within Sin3 and OSID (Opi1\u2013Sin3 interaction domain) in the N\u2010terminus of Opi1 were mapped as contact sites. The regulatory significance of the Opi1\u2013Sin3 interaction was shown by the obvious deregulation of an ICRE\u2010dependent reporter gene in a<jats:italic>sin3</jats:italic>mutant. Opi1 also interacts with a newly identified functional domain of the transcriptional activator Ino2 (RID, repressor interaction domain). These results define the molecular composition of the transcription complex mediating control of ICRE\u2010dependent genes and allow a hypothesis on the flow of regulatory information in response to phospholipid precursors.</jats:p>",
  "DOI": "10.1046/j.1365-2958.2001.02495.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        12
      ]
    ],
    "date-time": "2003-03-12T11:38:12Z",
    "timestamp": 1047469092000
  },
  "page": "155-166",
  "source": "Crossref",
  "is-referenced-by-count": 93,
  "title": "The negative regulator Opi1 of phospholipid biosynthesis in yeast contacts the pleiotropic repressor Sin3 and the transcriptional activator Ino2",
  "prefix": "10.1111",
  "volume": "41",
  "author": [
    {
      "given": "Christian",
      "family": "Wagner",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Martin",
      "family": "Dietz",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J\u00fcrgen",
      "family": "Wittmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Antje",
      "family": "Albrecht",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hans\u2010Joachim",
      "family": "Sch\u00fcller",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        12,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/387049a0"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90355-0"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.16.10.5772"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.42.25087"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/20.6.1411"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/154.2.573"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.10.10.1247"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.274.46.32750"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/28.16.3160"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00331845"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)82077-4"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/387043a0"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0079-6603(08)60826-0"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s0014-5793(97)01085-5"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/oxfordjournals.jbchem.a124287"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "crossref",
      "first-page": "4736",
      "DOI": "10.1016/S0021-9258(19)39624-3",
      "article-title": "The Saccharomyces cerevisiae INO4 gene encodes a small, highly basic protein required for derepression of phospholipid biosynthesis enzymes",
      "volume": "265",
      "author": "Hoshizaki D.K.",
      "year": "1990",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "475",
      "DOI": "10.1093/genetics/136.2.475",
      "article-title": "A pleiotropic phospholipid biosynthetic regulatory mutation in Saccharomyces cerevisiae is allelic to sin3 (sdi1, ume4, rpd1)",
      "volume": "136",
      "author": "Hudak K.A.",
      "year": "1994",
      "journal-title": "Genetics"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/144.4.1425"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80217-2"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.18.9.5121"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s004380050581"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.17.8.4852"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90146-4"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1997.5611937.x"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80215-9"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1093/oso/9780198502487.001.0001",
      "volume-title": "Helix\u2013Loop\u2013Helix Transcription Factors",
      "author": "Littlewood T.D.",
      "year": "1998"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.14.3987"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/19.7.1687"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.175.13.4235-4238.1993"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/22.25.5767"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90236-4"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/20.12.3253"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.25.14503"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(sici)1521-1878(199810)20:10<808::aid-bies6>3.3.co;2-l"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(95)90356-9"
    },
    {
      "key": "e_1_2_6_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1992.tb05033.x"
    },
    {
      "key": "e_1_2_6_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/20.22.5955"
    },
    {
      "key": "e_1_2_6_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(95)00818-t"
    },
    {
      "key": "e_1_2_6_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/23.2.230"
    },
    {
      "key": "e_1_2_6_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s002940050231"
    },
    {
      "key": "e_1_2_6_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/23.11.1964"
    },
    {
      "key": "e_1_2_6_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/s0968-0004(00)01592-9"
    },
    {
      "key": "e_1_2_6_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(87)90235-2"
    },
    {
      "key": "e_1_2_6_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.8.7.796"
    },
    {
      "key": "e_1_2_6_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/144.4.1343"
    },
    {
      "key": "e_1_2_6_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.11.12.6306"
    },
    {
      "key": "e_1_2_6_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.6.2370"
    },
    {
      "key": "e_1_2_6_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35044127"
    },
    {
      "key": "e_1_2_6_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/yea.320101310"
    },
    {
      "key": "e_1_2_6_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(sici)1097-0061(199907)15:10a<843::aid-yea424>3.3.co;2-d"
    },
    {
      "key": "e_1_2_6_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.13.3.1805"
    },
    {
      "key": "e_1_2_6_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.10.11.5927"
    },
    {
      "key": "e_1_2_6_54_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.829100"
    },
    {
      "key": "e_1_2_6_55_1",
      "doi-asserted-by": "crossref",
      "first-page": "863",
      "DOI": "10.1016/S0021-9258(17)35253-5",
      "article-title": "The OPI1 gene of Saccharomyces cerevisiae, a negative regulator of phospholipid biosynthesis, encodes a protein containing polyglutamine tracts and a leucine zipper",
      "volume": "266",
      "author": "White M.J.",
      "year": "1991",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_6_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.10.12.6500"
    },
    {
      "key": "e_1_2_6_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00587597"
    },
    {
      "key": "e_1_2_6_58_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80216-0"
    },
    {
      "key": "e_1_2_6_59_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80102-1"
    }
  ],
  "container-title": "Molecular Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.2001.02495.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2001.02495.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        9
      ]
    ],
    "date-time": "2024-01-09T08:42:02Z",
    "timestamp": 1704789722000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2001.02495.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        7
      ]
    ]
  },
  "references-count": 58,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2001,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.2001.02495.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.2001.02495.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2001,
        7
      ]
    ]
  }
}