DOI: 10.1046/j.1365-2958.2001.02476.x. The highly conserved domain of the <i>Caulobacter</i> McpA chemoreceptor is required for its polar localization

The highly conserved domain of the Caulobacter McpA chemoreceptor is required for its polar localization

10.1046/j.1365-2958.2001.02476.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

We have fused GFP to the C‐terminus of McpA to study chemoreceptor polar localization in Caulobacter crescentus. The full‐length McpA–GFP fusion is polarly localized and methylated. The methylation is dependent on the chemoreceptor methyltransferase (cheR) and chemoreceptor methylesterase (cheB) genes present in the mcpA operon. C‐terminal and internal deletions of McpA were constructed and fused to the N‐terminus of GFP to identify the domains required for polar localization. When the R1 methylation domain was deleted, the McpA–GFP fusion was still polarly localized, suggesting that this domain is dispensable for polar localization. However, when the highly conserved domain (HCD), which is involved in interacting with CheW, was deleted either by an internal deletion or C‐terminal deletion, the resulting McpA–GFP fusions were completely delocalized. When the mcpA operon, which contains the cheW and cheA homologues, was deleted, the full‐length McpA–GFP fusion was delocalized. Although additional chemotaxis genes are required for the polar localization of McpA–GFP, the presence of the single polar flagellum is not required. However, in filamentous cells, which are frequently found in C. crescentus fliF mutants, the McpA–GFP fusion was observed at mid‐cell positions.

Bibliography

Alley, M. R. K. (2001). The highly conserved domain of the Caulobacter McpA chemoreceptor is required for its polar localization. Molecular Microbiology, 40(6), 1335â1343. Portico.

Authors 1

M. R. K. Alley (first)

References 50 Referenced 18

10.1093/genetics/129.2.333
10.1101/gad.6.5.825
10.1126/science.8456303
10.1073/pnas.96.19.10667
10.1038/354161a0
10.1016/0378-1119(95)00685-0
10.1007/BF00332885
10.1128/JB.182.22.6499-6502.2000
10.1128/jb.175.8.2189-2196.1993
10.1016/0092-8674(78)90049-1
10.1111/j.1365-2958.1995.tb02274.x
10.1046/j.1365-2958.1997.6502022.x
10.1046/j.1365-2958.1999.01226.x
10.1073/pnas.91.26.12501
10.1016/0378-1119(89)90319-3
10.1128/jb.172.3.1327-1339.1990
10.1099/00221287-147-4-949
10.1073/pnas.80.12.3599
10.1038/23512
10.1046/j.1365-2958.2000.01676.x
10.1016/S0021-9258(19)74398-1 / J Biol Chem / Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis by Kirsch M.L. (1993)
10.1242/jcs.105.3.699
10.1016/0022-2836(84)90407-8
10.1073/pnas.71.8.3157
10.1128/jb.173.16.4941-4951.1991
10.1093/emboj/16.24.7231
10.1126/science.8456299
10.1128/JB.182.15.4337-4342.2000
10.1101/gad.12.21.3419
10.1128/jb.178.3.675-682.1996 / J Bacteriol / Flagellar assembly in Caulobacter crescentus: a basal body P‐ring null mutation affects stability of the l‐ring protein by Mohr C.D. (1996)
10.1128/JB.165.1.161-166.1986
10.1073/pnas.061029298
10.1073/pnas.89.21.10297
10.1128/jb.179.7.2169-2180.1997
10.1128/jb.151.1.106-113.1982
10.1016/0092-8674(78)90048-X
10.1016/S0092-8674(00)80455-9
10.1073/pnas.96.9.4971
10.1101/gad.4.3.344
10.1073/pnas.80.17.5262
10.1038/35041030
10.1046/j.1365-2958.2000.02044.x
10.1016/0378-1119(86)90117-4
10.1073/pnas.76.9.4350
10.1128/JB.182.2.504-507.2000
10.1073/pnas.90.2.630
10.1111/j.1365-2958.1995.mmi_17020357.x
10.1016/S1097-2765(00)80379-2
10.1021/bi9530189
10.1093/nar/24.22.4592

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:38 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 8:46 p.m.)
Indexed	1 year, 3 months ago (May 22, 2024, 9:15 a.m.)
Issued	24 years, 3 months ago (June 1, 2001)
Published	24 years, 3 months ago (June 1, 2001)
Published Online	23 years, 8 months ago (Dec. 21, 2001)
Published Print	24 years, 3 months ago (June 1, 2001)

Funders 0

None

BibTeX

@article{Alley_2001, title={The highly conserved domain of the Caulobacter McpA chemoreceptor is required for its polar localization}, volume={40}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2001.02476.x}, DOI={10.1046/j.1365-2958.2001.02476.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Alley, M. R. K.}, year={2001}, month=jun, pages={1335–1343} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        5,
        22
      ]
    ],
    "date-time": "2024-05-22T13:15:31Z",
    "timestamp": 1716383731679
  },
  "reference-count": 50,
  "publisher": "Wiley",
  "issue": "6",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            12,
            21
          ]
        ],
        "date-time": "2001-12-21T00:00:00Z",
        "timestamp": 1008892800000
      },
      "content-version": "vor",
      "delay-in-days": 203,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  },
  "abstract": "<jats:p>We have fused GFP to the C\u2010terminus of McpA to study chemoreceptor polar localization in <jats:italic>Caulobacter crescentus</jats:italic>. The full\u2010length McpA\u2013GFP fusion is polarly localized and methylated. The methylation is dependent on the chemoreceptor methyltransferase (<jats:italic>cheR</jats:italic>) and chemoreceptor methylesterase (<jats:italic>cheB</jats:italic>) genes present in the <jats:italic>mcpA</jats:italic> operon. C\u2010terminal and internal deletions of McpA were constructed and fused to the N\u2010terminus of GFP to identify the domains required for polar localization. When the R1 methylation domain was deleted, the McpA\u2013GFP fusion was still polarly localized, suggesting that this domain is dispensable for polar localization. However, when the highly conserved domain (HCD), which is involved in interacting with CheW, was deleted either by an internal deletion or C\u2010terminal deletion, the resulting McpA\u2013GFP fusions were completely delocalized. When the <jats:italic>mcpA</jats:italic> operon, which contains the <jats:italic>cheW and cheA</jats:italic> homologues, was deleted, the full\u2010length McpA\u2013GFP fusion was delocalized. Although additional chemotaxis genes are required for the polar localization of McpA\u2013GFP, the presence of the single polar flagellum is not required. However, in filamentous cells, which are frequently found in <jats:italic>C. crescentus fliF</jats:italic> mutants, the McpA\u2013GFP fusion was observed at mid\u2010cell positions.</jats:p>",
  "DOI": "10.1046/j.1365-2958.2001.02476.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        12
      ]
    ],
    "date-time": "2003-03-12T11:38:44Z",
    "timestamp": 1047469124000
  },
  "page": "1335-1343",
  "source": "Crossref",
  "is-referenced-by-count": 18,
  "title": "The highly conserved domain of the <i>Caulobacter</i> McpA chemoreceptor is required for its polar localization",
  "prefix": "10.1111",
  "volume": "40",
  "author": [
    {
      "given": "M. R. K.",
      "family": "Alley",
      "sequence": "first",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        12,
        21
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/genetics/129.2.333"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.6.5.825"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8456303"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.19.10667"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/354161a0"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(95)00685-0"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00332885"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.182.22.6499-6502.2000"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.175.8.2189-2196.1993"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(78)90049-1"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1995.tb02274.x"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1997.6502022.x"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1999.01226.x"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.91.26.12501"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(89)90319-3"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.172.3.1327-1339.1990"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/00221287-147-4-949"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.80.12.3599"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/23512"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2000.01676.x"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "crossref",
      "first-page": "25350",
      "DOI": "10.1016/S0021-9258(19)74398-1",
      "article-title": "Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis",
      "volume": "268",
      "author": "Kirsch M.L.",
      "year": "1993",
      "journal-title": "J Biol Chem"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1242/jcs.105.3.699"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0022-2836(84)90407-8"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.71.8.3157"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.173.16.4941-4951.1991"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.24.7231"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8456299"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.182.15.4337-4342.2000"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.12.21.3419"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "crossref",
      "first-page": "675",
      "DOI": "10.1128/jb.178.3.675-682.1996",
      "article-title": "Flagellar assembly in Caulobacter crescentus: a basal body P\u2010ring null mutation affects stability of the l\u2010ring protein",
      "volume": "178",
      "author": "Mohr C.D.",
      "year": "1996",
      "journal-title": "J Bacteriol"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.165.1.161-166.1986"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.061029298"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.21.10297"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.179.7.2169-2180.1997"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.151.1.106-113.1982"
    },
    {
      "key": "e_1_2_6_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(78)90048-X"
    },
    {
      "key": "e_1_2_6_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)80455-9"
    },
    {
      "key": "e_1_2_6_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.9.4971"
    },
    {
      "key": "e_1_2_6_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.4.3.344"
    },
    {
      "key": "e_1_2_6_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.80.17.5262"
    },
    {
      "key": "e_1_2_6_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35041030"
    },
    {
      "key": "e_1_2_6_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2000.02044.x"
    },
    {
      "key": "e_1_2_6_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(86)90117-4"
    },
    {
      "key": "e_1_2_6_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.76.9.4350"
    },
    {
      "key": "e_1_2_6_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.182.2.504-507.2000"
    },
    {
      "key": "e_1_2_6_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.2.630"
    },
    {
      "key": "e_1_2_6_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1995.mmi_17020357.x"
    },
    {
      "key": "e_1_2_6_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1097-2765(00)80379-2"
    },
    {
      "key": "e_1_2_6_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi9530189"
    },
    {
      "key": "e_1_2_6_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/24.22.4592"
    }
  ],
  "container-title": "Molecular Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.2001.02476.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2001.02476.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        28
      ]
    ],
    "date-time": "2023-09-28T00:46:54Z",
    "timestamp": 1695862014000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2001.02476.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          2001,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.2001.02476.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.2001.02476.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2001,
        6
      ]
    ]
  }
}