DOI: 10.1046/j.1365-2958.2001.02200.x. Structure and composition of the <i>Shigella flexneri</i>‘needle complex’, a part of its type III secreton

Structure and composition of the Shigella flexneri‘needle complex’, a part of its type III secreton

10.1046/j.1365-2958.2001.02200.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Type III secretion systems (TTSSs or secretons), essential virulence determinants of many Gram‐negative bacteria, serve to translocate proteins directly from the bacteria into the host cytoplasm. Electron microscopy (EM) indicates that the TTSSs of Shigella flexneri are composed of: (1) an external needle; (2) a transmembrane domain; and (3) a cytoplasmic bulb. EM analysis of purified and negatively stained parts 1, 2 and a portion of 3 of the TTSS, together termed the ‘needle complex’ (NC), produced an average image at 17 Å resolution in which a base, an outer ring and a needle, inserted through the ring into the base, could be discerned. This analysis and cryoEM images of NCs indicated that the needle and base contain a central 2–3 nm canal. Five major NC components, MxiD, MxiG, MxiJ, MxiH and MxiI, were identified by N‐terminal sequencing. MxiG and MxiJ are predicted to be inner membrane proteins and presumably form the base. MxiD is predicted to be an outer membrane protein and to form the outer ring. MxiH and MxiI are small hydrophilic proteins. Mutants lacking either of these proteins formed needleless secretons and were unable to secrete Ipa proteins. As MxiH was present in NCs in large molar excess, we propose that it is the major needle component. MxiI may cap at the external needle tip.

Bibliography

Blocker, A., Jouihri, N., Larquet, E., Gounon, P., Ebel, F., Parsot, C., Sansonetti, P., & Allaoui, A. (2001). Structure and composition of the Shigella flexneriâneedle complexâ, a part of its type III secreton. Molecular Microbiology, 39(3), 652â663. Portico.

Authors 8

Ariel Blocker (first)
Noureddine Jouihri (additional)
Eric Larquet (additional)
Pierre Gounon (additional)
Frank Ebel (additional)
Claude Parsot (additional)
Philippe Sansonetti (additional)
Abdelmounaaïm Allaoui (additional)

References 58 Referenced 291

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:35 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 6:54 p.m.)
Indexed	3 weeks, 6 days ago (July 25, 2025, 5:57 a.m.)
Issued	24 years, 6 months ago (Feb. 1, 2001)
Published	24 years, 6 months ago (Feb. 1, 2001)
Published Online	23 years, 8 months ago (Dec. 21, 2001)
Published Print	24 years, 6 months ago (Feb. 1, 2001)

Funders 0

None

BibTeX

@article{Blocker_2001, title={Structure and composition of the Shigella flexneri‘needle complex’, a part of its type III secreton}, volume={39}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2001.02200.x}, DOI={10.1046/j.1365-2958.2001.02200.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Blocker, Ariel and Jouihri, Noureddine and Larquet, Eric and Gounon, Pierre and Ebel, Frank and Parsot, Claude and Sansonetti, Philippe and Allaoui, Abdelmounaaïm}, year={2001}, month=feb, pages={652–663} }

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