DOI: 10.1046/j.1365-2958.2000.02170.x. The <i>txtAB</i> genes of the plant pathogen <i>Streptomyces acidiscabies</i> encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity

The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity

10.1046/j.1365-2958.2000.02170.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Four Streptomyces species have been described as the causal agents of scab disease, which affects economically important root and tuber crops worldwide. These species produce a family of cyclic dipeptides, the thaxtomins, which alone mimic disease symptomatology. Structural considerations suggest that thaxtomins are synthesized non‐ribosomally. Degenerate oligonucleotide primers were used to amplify conserved portions of the acyladenylation module of peptide synthetase genes from genomic DNA of representatives of the four species. Pairwise Southern hybridizations identified a peptide synthetase acyladenylation module conserved among three species. The complete nucleotide sequences of two peptide synthetase genes (txtAB) were determined from S. acidiscabies 84.104 cosmid library clones. The organization of the deduced TxtA and TxtB peptide synthetase catalytic domains is consistent with the formation of N‐methylated cyclic dipeptides such as thaxtomins. Based on high‐performance liquid chromatography (HPLC) analysis, thaxtomin A production was abolished in txtA gene disruption mutants. Although the growth and morphological characteristics of the mutants were identical to those of the parent strain, txtA mutants were avirulent on potato tubers. Moreover, introduction of the thaxtomin synthetase cosmid into a txtA mutant restored both pathogenicity and thaxtomin A production, demonstrating a critical role for thaxtomins in pathogenesis.

Bibliography

Healy, F. G., Wach, M., Krasnoff, S. B., Gibson, D. M., & Loria, R. (2000). The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity. Molecular Microbiology, 38(4), 794â804. Portico.

Authors 5

Frank G. Healy (first)
Michael Wach (additional)
Stuart B. Krasnoff (additional)
Donna M. Gibson (additional)
Rosemary Loria (additional)

References 45 Referenced 190

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{'key': 'e_1_2_6_7_1', 'first-page': '201', 'article-title': 'Highly conserved‐methyltransferases as an integral part of peptide synthetases', 'volume': '37', 'author': 'Burmester J.', 'year': '1995', 'journal-title': 'Biochem Mol Biol Int'} / Biochem Mol Biol Int / Highly conserved‐methyltransferases as an integral part of peptide synthetases by Burmester J. (1995)
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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 4:23 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 2, 2025, 1:10 a.m.)
Issued	24 years, 9 months ago (Nov. 1, 2000)
Published	24 years, 9 months ago (Nov. 1, 2000)
Published Online	23 years, 7 months ago (Jan. 5, 2002)
Published Print	24 years, 9 months ago (Nov. 1, 2000)

Funders 0

None

BibTeX

@article{Healy_2000, title={The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin A production and pathogenicity}, volume={38}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.02170.x}, DOI={10.1046/j.1365-2958.2000.02170.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Healy, Frank G. and Wach, Michael and Krasnoff, Stuart B. and Gibson, Donna M. and Loria, Rosemary}, year={2000}, month=nov, pages={794–804} }

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