DOI: 10.1046/j.1365-2958.2000.02135.x. The R‐type pyocin of <i>Pseudomonas aeruginosa</i> is related to P2 phage, and the F‐type is related to lambda phage

The R‐type pyocin of Pseudomonas aeruginosa is related to P2 phage, and the F‐type is related to lambda phage

10.1046/j.1365-2958.2000.02135.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Pseudomonas aeruginosa produces three types of bacteriocins: R‐, F‐ and S‐type pyocins. The S‐type pyocin is a colicin‐like protein, whereas the R‐type pyocin resembles a contractile but non‐flexible tail structure of bacteriophage, and the F‐type a flexible but non‐contractile one. As genetically related phages exist for each type, these pyocins have been thought to be variations of defective phage. In the present study, the nucleotide sequence of R2 pyocin genes, along with those for F2 pyocin, which are located downstream of the R2 gene cluster on the chromosome of P. aeruginosa PAO1, was analysed in order to elucidate the relationship between the pyocins and bacteriophages. The results clearly demonstrated that the R‐type pyocin is derived from a common ancestral origin with P2 phage and the F‐type from λ phage. This notion was supported by identification of a lysis gene cassette similar to those for bacteriophages. The gene organization of the R2 and F2 pyocin gene cluster, however, suggested that both pyocins are not simple defective phages, but are phage tails that have been evolutionarily specialized as bacteriocins. A systematic polymerase chain reaction (PCR) analysis of P. aeruginosa strains that produce various subtypes of R and F pyocins revealed that the genes for every subtype are located between trpE and trpG in the same or very similar gene organization as for R2 and F2 pyocins, but with alterations in genes that determine the receptor specificity.

Bibliography

Nakayama, K., Takashima, K., Ishihara, H., Shinomiya, T., Kageyama, M., Kanaya, S., Ohnishi, M., Murata, T., Mori, H., & Hayashi, T. (2000). The Râtype pyocin of Pseudomonas aeruginosa is related to P2 phage, and the Fâtype is related to lambda phage. Molecular Microbiology, 38(2), 213â231. Portico.

Authors 10

Keisuke Nakayama (first)
Kayoko Takashima (additional)
Hiroshi Ishihara (additional)
Tomoyuki Shinomiya (additional)
Makoto Kageyama (additional)
Shigehiko Kanaya (additional)
Makoto Ohnishi (additional)
Takahiro Murata (additional)
Hirotada Mori (additional)
Tetsuya Hayashi (additional)

References 59 Referenced 303

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 9:29 a.m.)
Indexed	3 days, 17 hours ago (Aug. 27, 2025, 12:08 p.m.)
Issued	24 years, 10 months ago (Oct. 1, 2000)
Published	24 years, 10 months ago (Oct. 1, 2000)
Published Online	23 years, 7 months ago (Jan. 18, 2002)
Published Print	24 years, 10 months ago (Oct. 1, 2000)

Funders 0

None

BibTeX

@article{Nakayama_2000, title={The R‐type pyocin of Pseudomonas aeruginosa is related to P2 phage, and the F‐type is related to lambda phage}, volume={38}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.02135.x}, DOI={10.1046/j.1365-2958.2000.02135.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Nakayama, Keisuke and Takashima, Kayoko and Ishihara, Hiroshi and Shinomiya, Tomoyuki and Kageyama, Makoto and Kanaya, Shigehiko and Ohnishi, Makoto and Murata, Takahiro and Mori, Hirotada and Hayashi, Tetsuya}, year={2000}, month=oct, pages={213–231} }

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