DOI: 10.1046/j.1365-2958.2000.02037.x. <i>Entamoeba histolytica</i> cysteine proteinases with interleukin‐1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis

Entamoeba histolytica cysteine proteinases with interleukin‐1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis

10.1046/j.1365-2958.2000.02037.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

The protozoan parasite Entamoeba histolytica causes intestinal inflammation and ulceration. Amoebic trophozoites activate the transcription factor NF‐κB in human intestinal epithelial cells, initiating an inflammatory response programme with resultant damage to the intestinal tissue. Amoebic cysteine proteinases have been proposed as important virulence factors for amoebiasis. To test the role of amoebic cysteine proteinases in the pathogenesis of amoebic colitis, human intestinal xenografts in SCID mice were infected with E. histolytica trophozoites expressing an antisense message to ehcp5. The cysteine proteinase‐deficient amoeba failed to induce intestinal epithelial cell production of the inflammatory cytokines interleukin (IL)‐1B and IL‐8, and caused significantly less gut inflammation and damage to the intestinal permeability barrier. The critical role of amoebic cysteine proteinases in human gut inflammation and tissue damage may be explained by our discovery that amoebic cysteine proteinases possess IL‐1B converting enzyme (ICE) activity. This ICE activity could contribute to intestinal inflammation by activating human pIL‐1B released by damaged intestinal cells. These results demonstrate for the first time that amoebic cysteine proteinases are a key virulence factor in amoebic colitis, and provide a novel mechanism for their activity.

Bibliography

Zhang, Z., Wang, L., Seydel, K. B., Li, E., Ankri, S., Mirelman, D., & Stanley, S. L. (2000). Entamoeba histolytica cysteine proteinases with interleukinâ1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis. Molecular Microbiology, 37(3), 542â548. Portico.

Authors 8

Zhi Zhang (first)
(additional)
Lei Wang (additional)
Karl B. Seydel (additional)
Ellen Li (additional)
Serge Ankri (additional)
David Mirelman (additional)
Samuel L. Stanley (additional)

References 23 Referenced 117

10.1046/j.1365-2958.1998.00837.x
10.1128/IAI.67.1.421-422.1999
10.1046/j.1365-2958.1999.01607.x
10.1046/j.1365-2958.1996.00111.x
10.1016/S0167-7799(97)01110-4
10.1073/pnas.88.19.8485
10.1080/00034983.1964.11686259
10.1136/bmj.2.5657.613
10.1073/pnas.90.16.7676
10.1084/jem.163.3.536
10.1016/s0169-4758(97)01038-7
10.1128/iai.63.10.4150-4153.1995 / Infect Immun / Interaction of laminin with Entamoeba histolytica cysteine proteinases and its effect on amebic pathogenesis by Li E. (1995)
10.1084/jem.152.2.377
{'key': 'e_1_2_6_15_1', 'first-page': '115', 'article-title': 'On the specificity of a cysteine proteinase from Entamoeba histolytica', 'volume': '47', 'author': 'Scholze H.', 'year': '1988', 'journal-title': 'Biomed Biochim Acta'} / Biomed Biochim Acta / On the specificity of a cysteine proteinase from Entamoeba histolytica by Scholze H. (1988)
10.1111/j.1550-7408.1989.tb01092.x
10.1128/IAI.66.6.2980-2983.1998 / Infect Immun / Entamoeba histolytica induces host cell death in amebic liver abscess by a non‐Fas‐dependent, non‐tumor necrosis factor alpha‐dependent pathway of apoptosis by Seydel K.B. (1998)
10.1128/iai.65.5.1631-1639.1997
10.1016/S0016-5085(98)70023-X
{'key': 'e_1_2_6_20_1', 'first-page': '214', 'article-title': 'Pathophysiology of amebiasis', 'volume': '4', 'author': 'Stanley S.L.', 'year': '1993', 'journal-title': 'Sem Gastrointest Dis'} / Sem Gastrointest Dis / Pathophysiology of amebiasis by Stanley S.L. (1993)
10.1128/iai.63.4.1587-1590.1995 / Infect Immun / Role of the Entamoeba histolytica cysteine proteinase in amebic liver abscess formation in severe combined immunodeficient (SCID) mice by Stanley S.L. (1995)
10.1038/356768a0
10.1001/archinte.1975.00330050055009 / Arch Intern Med / Amebic colitis mistaken for inflammatory bowel disease by Tucker P.C. (1975)
10.1093/clinids/8.2.228

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:32 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 12:34 p.m.)
Indexed	2 months, 2 weeks ago (June 20, 2025, 2:06 p.m.)
Issued	25 years, 1 month ago (Aug. 1, 2000)
Published	25 years, 1 month ago (Aug. 1, 2000)
Published Online	23 years, 7 months ago (Jan. 18, 2002)
Published Print	25 years, 1 month ago (Aug. 1, 2000)

Funders 0

None

BibTeX

@article{Zhang_2000, title={Entamoeba histolytica cysteine proteinases with interleukin‐1 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis}, volume={37}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.02037.x}, DOI={10.1046/j.1365-2958.2000.02037.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Zhang, Zhi and Wang, Lei and Seydel, Karl B. and Li, Ellen and Ankri, Serge and Mirelman, David and Stanley, Samuel L.}, year={2000}, month=aug, pages={542–548} }

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