DOI: 10.1046/j.1365-2958.2000.01987.x. Iron acquisition and virulence in <i>Helicobacter pylori</i>: a major role for FeoB, a high‐affinity ferrous iron transporter

Iron acquisition and virulence in Helicobacter pylori: a major role for FeoB, a high‐affinity ferrous iron transporter

10.1046/j.1365-2958.2000.01987.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

The genome sequence of Helicobacter pylori suggests that this bacterium possesses several Fe acquisition systems, including both Fe2+‐ and Fe3+‐citrate transporters. The role of these transporters was investigated by generating insertion mutants in feoB, tonB, fecA1 and fecDE. Fe transport in the feoB mutant was ≈ 10‐fold lower than in the wild type (with 0.5 μM Fe), irrespective of whether Fe was supplied in the Fe2+ or Fe3+ form. In contrast, transport rates were unaffected by the other mutations. Complementation of the feoB mutation fully restored both Fe2+ and Fe3+ transport. The growth inhibition exhibited by the feoB mutant in Fe‐deficient media was relieved by human holo‐transferrin, holo‐lactoferrin and Fe3+‐dicitrate, but not by FeSO4. The feoB mutant had less cellular Fe and was more sensitive to growth inhibition by transition metals in comparison with the wild type. Biphasic kinetics of Fe2+ transport in the wild type suggested the presence of high‐ and low‐affinity uptake systems. The high‐affinity system (apparent Ks = 0.54 μM) is absent in the feoB mutant. Transport via FeoB is highly specific for Fe2+ and was inhibited by FCCP, DCCD and vanadate, indicating an active process energized by ATP. Ferrozine inhibition of Fe2+ and Fe3+ uptake implied the concerted involvement of both an Fe3+ reductase and FeoB in the uptake of Fe supplied as Fe3+. Taken together, the results are consistent with FeoB‐mediated Fe2+ uptake being a major pathway for H. pylori Fe acquisition. feoB mutants were unable to colonize the gastric mucosa of mice, indicating that FeoB makes an important contribution to Fe acquisition by H. pylori in the low‐pH, low‐O2 environment of the stomach.

Bibliography

Velayudhan, J., Hughes, N. J., McColm, A. A., Bagshaw, J., Clayton, C. L., Andrews, S. C., & Kelly, D. J. (2000). Iron acquisition and virulence in Helicobacter pylori: a major role for FeoB, a highâaffinity ferrous iron transporter. Molecular Microbiology, 37(2), 274â286. Portico.

Authors 7

Jyoti Velayudhan (first)
Nicky J. Hughes (additional)
Andrew A. McColm (additional)
Julie Bagshaw (additional)
Chris L. Clayton (additional)
Simon C. Andrews (additional)
David J. Kelly (additional)

References 44 Referenced 237

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:32 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 3:37 a.m.)
Indexed	2 days, 2 hours ago (Sept. 4, 2025, 10:28 a.m.)
Issued	25 years, 2 months ago (July 1, 2000)
Published	25 years, 2 months ago (July 1, 2000)
Published Online	22 years, 8 months ago (Dec. 11, 2002)
Published Print	25 years, 2 months ago (July 1, 2000)

Funders 0

None

BibTeX

@article{Velayudhan_2000, title={Iron acquisition and virulence in Helicobacter pylori: a major role for FeoB, a high‐affinity ferrous iron transporter}, volume={37}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.01987.x}, DOI={10.1046/j.1365-2958.2000.01987.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Velayudhan, Jyoti and Hughes, Nicky J. and McColm, Andrew A. and Bagshaw, Julie and Clayton, Chris L. and Andrews, Simon C. and Kelly, David J.}, year={2000}, month=jul, pages={274–286} }

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