DOI: 10.1046/j.1365-2958.2000.01913.x. Regulation of quorum sensing in <i>Vibrio harveyi</i> by LuxO and Sigma‐54

Regulation of quorum sensing in Vibrio harveyi by LuxO and Sigma‐54

10.1046/j.1365-2958.2000.01913.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

The bioluminescent marine bacterium Vibrio harveyi controls light production (lux) by an elaborate quorum‐sensing circuit. V. harveyi produces and responds to two different autoinducer signals (AI‐1 and AI‐2) to modulate the luciferase structural operon (luxCDABEGH) in response to changes in cell‐population density. Unlike all other Gram‐negative quorum‐sensing organisms, V. harveyi regulates quorum sensing using a two‐component phosphorylation–dephosphorylation cascade. Each autoinducer is recognized by a cognate hybrid sensor kinase (called LuxN and LuxQ). Both sensors transduce information to a shared phosphorelay protein called LuxU, which in turn conveys the signal to the response regulator protein LuxO. Phospho‐LuxO is responsible for repression of luxCDABEGH expression at low cell density. In the present study, we demonstrate that LuxO functions as an activator protein via interaction with the alternative sigma factor, σ54 (encoded by rpoN). Our results suggest that LuxO, together with σ54, activates the expression of a negative regulator of luminescence. We also show that phenotypes other than lux are regulated by LuxO and σ54, demonstrating that in Vibrio harveyi, quorum sensing controls multiple processes.

Bibliography

Lilley, B. N., & Bassler, B. L. (2000). Regulation of quorum sensing in Vibrio harveyi by LuxO and Sigmaâ54. Molecular Microbiology, 36(4), 940â954. Portico.

Authors 2

Brendan N. Lilley (first)
Bonnie L. Bassler (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:31 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 11:17 a.m.)
Indexed	1 day, 5 hours ago (Aug. 29, 2025, 5:51 a.m.)
Issued	25 years, 3 months ago (May 1, 2000)
Published	25 years, 3 months ago (May 1, 2000)
Published Online	23 years, 7 months ago (Jan. 18, 2002)
Published Print	25 years, 3 months ago (May 1, 2000)

Funders 0

None

BibTeX

@article{Lilley_2000, title={Regulation of quorum sensing in Vibrio harveyi by LuxO and Sigma‐54}, volume={36}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.01913.x}, DOI={10.1046/j.1365-2958.2000.01913.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Lilley, Brendan N. and Bassler, Bonnie L.}, year={2000}, month=may, pages={940–954} }

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