DOI: 10.1046/j.1365-2958.2000.01882.x. Oxygenated mycolic acids are necessary for virulence of <i>Mycobacterium tuberculosis</i> in mice

Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice

10.1046/j.1365-2958.2000.01882.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Members of the Mycobacterium tuberculosis group synthesize a family of long‐chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non‐oxygenated α‐mycolic acid and the oxygenated keto‐ and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.

Bibliography

Dubnau, E., Chan, J., Raynaud, C., Mohan, V. P., LanÃ©elle, M., Yu, K., QuÃ©mard, A., Smith, I., & DaffÃ©, M. (2000). Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice. Molecular Microbiology, 36(3), 630â637. Portico.

Authors 9

Eugenie Dubnau (first)
John Chan (additional)
Catherine Raynaud (additional)
Vellore P. Mohan (additional)
Marie‐Antoinette Lanéelle (additional)
Keming Yu (additional)
Annaik Quémard (additional)
Issar Smith (additional)
Mamadou Daffé (additional)

References 34 Referenced 260

10.1128/IAI.68.2.767-778.2000
10.1126/science.276.5317.1420
{'key': 'e_1_2_6_4_2', 'first-page': '203', 'volume-title': 'Microbial Lipids.', 'author': 'Brennan P.J.', 'year': '1988'} / Microbial Lipids. by Brennan P.J. (1988)
10.1046/j.1365-2958.1999.01593.x
10.1128/IAI.63.2.736-740.1995
10.1038/47042
10.1016/S0065-2911(08)60016-8
10.1016/S0769-2609(83)80037-4
10.1016/S0022-2275(20)40272-X / J Lipid Res / A simple specific spray for the detection of phospholipids on thin layer chromatography by Dittmer J.C.F. (1964)
10.1046/j.1365-2958.1997.2301589.x
{'key': 'e_1_2_6_12_2', 'first-page': '1526', 'article-title': 'Mutations in the cmaB gene are responsible for the absence of methoxymycolic acid in Mycobacterium bovis BCG Pasteur', 'volume': '29', 'author': 'Dubnau E.', 'year': '1998', 'journal-title': 'Mol Microbiol'} / Mol Microbiol / Mutations in the cmaB gene are responsible for the absence of methoxymycolic acid in Mycobacterium bovis BCG Pasteur by Dubnau E. (1998)
10.1074/jbc.270.45.27292
{'key': 'e_1_2_6_14_2', 'volume-title': 'Tuberculosis.', 'author': 'Goren M.B.', 'year': '1979'} / Tuberculosis. by Goren M.B. (1979)
10.1046/j.1365-2958.1999.01310.x
10.1016/0076-6879(91)04027-L
10.1128/jb.172.3.1418-1423.1990
10.1111/j.1574-6968.1994.tb07194.x
10.1111/j.1365-2958.1992.tb01410.x
10.1073/pnas.92.24.11254
10.1074/jbc.271.47.29545
{'key': 'e_1_2_6_22_2', 'first-page': '941', 'article-title': 'Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini‐circle', 'volume': '135', 'author': 'Lydiate D.J.', 'year': '1989', 'journal-title': 'J Gen Microbiol'} / J Gen Microbiol / Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini‐circle by Lydiate D.J. (1989)
{'key': 'e_1_2_6_23_2', 'volume-title': 'Lipids: Complex Lipids, Their Chemistry, Biosynthesis and Roles.', 'author': 'Minnikin D.E.', 'year': '1982'} / Lipids: Complex Lipids, Their Chemistry, Biosynthesis and Roles. by Minnikin D.E. (1982)
10.1111/j.1365-2958.1993.tb01647.x
10.1128/jb.178.4.1197-1199.1996
{'key': 'e_1_2_6_26_2', 'first-page': '000', 'article-title': 'Characterization of the in vivo acceptors of the mycoloyl residues transferred by the corynebacterial PS1 and the related mycobacterial antigens 85', 'volume': '36', 'author': 'Puech V.', 'year': '2000', 'journal-title': 'Mol Microbiol'} / Mol Microbiol / Characterization of the in vivo acceptors of the mycoloyl residues transferred by the corynebacterial PS1 and the related mycobacterial antigens 85 by Puech V. (2000)
10.1111/j.1432-1033.1997.00758.x
{'key': 'e_1_2_6_28_2', 'first-page': '547', 'volume-title': 'The Mycobacteria: A Source Book, Part A.', 'author': 'Segal W.', 'year': '1984'} / The Mycobacteria: A Source Book, Part A. by Segal W. (1984)
10.1128/jb.72.2.132-141.1956
10.1038/351456a0
{'key': 'e_1_2_6_31_2', 'first-page': '20', 'article-title': 'Aerosol infection of mice with mycobacteria using a nose‐only exposure device', 'volume': '2', 'author': 'Tsenova L.', 'year': '1997', 'journal-title': 'J Am Biol Safety Assoc'} / J Am Biol Safety Assoc / Aerosol infection of mice with mycobacteria using a nose‐only exposure device by Tsenova L. (1997)
{'key': 'e_1_2_6_32_2', 'first-page': '1530', 'article-title': 'Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester', 'volume': '42', 'author': 'Tsuchiya S.', 'year': '1982', 'journal-title': 'Cancer Res'} / Cancer Res / Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester by Tsuchiya S. (1982)
10.1073/pnas.93.23.12828
10.1074/jbc.272.15.10041
10.1046/j.1365-2958.1998.01026.x

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:30 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 3:01 a.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 5:24 a.m.)
Issued	25 years, 4 months ago (May 1, 2000)
Published	25 years, 4 months ago (May 1, 2000)
Published Online	23 years, 7 months ago (Jan. 18, 2002)
Published Print	25 years, 4 months ago (May 1, 2000)

Funders 0

None

BibTeX

@article{Dubnau_2000, title={Oxygenated mycolic acids are necessary for virulence of Mycobacterium tuberculosis in mice}, volume={36}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.01882.x}, DOI={10.1046/j.1365-2958.2000.01882.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Dubnau, Eugenie and Chan, John and Raynaud, Catherine and Mohan, Vellore P. and Lanéelle, Marie‐Antoinette and Yu, Keming and Quémard, Annaik and Smith, Issar and Daffé, Mamadou}, year={2000}, month=may, pages={630–637} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        26
      ]
    ],
    "date-time": "2025-07-26T09:24:19Z",
    "timestamp": 1753521859209
  },
  "reference-count": 34,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2002,
            1,
            18
          ]
        ],
        "date-time": "2002-01-18T00:00:00Z",
        "timestamp": 1011312000000
      },
      "content-version": "vor",
      "delay-in-days": 627,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2000,
        5
      ]
    ]
  },
  "abstract": "<jats:p>Members of the <jats:italic>Mycobacterium tuberculosis</jats:italic> group synthesize a family of long\u2010chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non\u2010oxygenated \u03b1\u2010mycolic acid and the oxygenated keto\u2010 and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of <jats:italic>M. tuberculosis</jats:italic> with an inactivated <jats:italic>hma</jats:italic> (<jats:italic>cmaA</jats:italic>, <jats:italic>mma4</jats:italic>) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice.</jats:p>",
  "DOI": "10.1046/j.1365-2958.2000.01882.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        12
      ]
    ],
    "date-time": "2003-03-12T11:30:55Z",
    "timestamp": 1047468655000
  },
  "page": "630-637",
  "source": "Crossref",
  "is-referenced-by-count": 260,
  "title": "Oxygenated mycolic acids are necessary for virulence of <i>Mycobacterium tuberculosis</i> in mice",
  "prefix": "10.1111",
  "volume": "36",
  "author": [
    {
      "given": "Eugenie",
      "family": "Dubnau",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "John",
      "family": "Chan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Catherine",
      "family": "Raynaud",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Vellore P.",
      "family": "Mohan",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Marie\u2010Antoinette",
      "family": "Lan\u00e9elle",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Keming",
      "family": "Yu",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Annaik",
      "family": "Qu\u00e9mard",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Issar",
      "family": "Smith",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Mamadou",
      "family": "Daff\u00e9",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2002,
        1,
        18
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/IAI.68.2.767-778.2000"
    },
    {
      "key": "e_1_2_6_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.276.5317.1420"
    },
    {
      "key": "e_1_2_6_4_2",
      "first-page": "203",
      "volume-title": "Microbial Lipids.",
      "author": "Brennan P.J.",
      "year": "1988"
    },
    {
      "key": "e_1_2_6_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1999.01593.x"
    },
    {
      "key": "e_1_2_6_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/IAI.63.2.736-740.1995"
    },
    {
      "key": "e_1_2_6_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/47042"
    },
    {
      "key": "e_1_2_6_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0065-2911(08)60016-8"
    },
    {
      "key": "e_1_2_6_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0769-2609(83)80037-4"
    },
    {
      "key": "e_1_2_6_10_2",
      "doi-asserted-by": "crossref",
      "first-page": "126",
      "DOI": "10.1016/S0022-2275(20)40272-X",
      "article-title": "A simple specific spray for the detection of phospholipids on thin layer chromatography",
      "volume": "5",
      "author": "Dittmer J.C.F.",
      "year": "1964",
      "journal-title": "J Lipid Res"
    },
    {
      "key": "e_1_2_6_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1997.2301589.x"
    },
    {
      "key": "e_1_2_6_12_2",
      "first-page": "1526",
      "article-title": "Mutations in the cmaB gene are responsible for the absence of methoxymycolic acid in Mycobacterium bovis BCG Pasteur",
      "volume": "29",
      "author": "Dubnau E.",
      "year": "1998",
      "journal-title": "Mol Microbiol"
    },
    {
      "key": "e_1_2_6_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.270.45.27292"
    },
    {
      "key": "e_1_2_6_14_2",
      "volume-title": "Tuberculosis.",
      "author": "Goren M.B.",
      "year": "1979"
    },
    {
      "key": "e_1_2_6_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1999.01310.x"
    },
    {
      "key": "e_1_2_6_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0076-6879(91)04027-L"
    },
    {
      "key": "e_1_2_6_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.172.3.1418-1423.1990"
    },
    {
      "key": "e_1_2_6_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1574-6968.1994.tb07194.x"
    },
    {
      "key": "e_1_2_6_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1992.tb01410.x"
    },
    {
      "key": "e_1_2_6_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.24.11254"
    },
    {
      "key": "e_1_2_6_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.271.47.29545"
    },
    {
      "key": "e_1_2_6_22_2",
      "first-page": "941",
      "article-title": "Physical and genetic characterization of chromosomal copies of the Streptomyces coelicolor mini\u2010circle",
      "volume": "135",
      "author": "Lydiate D.J.",
      "year": "1989",
      "journal-title": "J Gen Microbiol"
    },
    {
      "key": "e_1_2_6_23_2",
      "volume-title": "Lipids: Complex Lipids, Their Chemistry, Biosynthesis and Roles.",
      "author": "Minnikin D.E.",
      "year": "1982"
    },
    {
      "key": "e_1_2_6_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.1993.tb01647.x"
    },
    {
      "key": "e_1_2_6_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.178.4.1197-1199.1996"
    },
    {
      "key": "e_1_2_6_26_2",
      "first-page": "000",
      "article-title": "Characterization of the in vivo acceptors of the mycoloyl residues transferred by the corynebacterial PS1 and the related mycobacterial antigens 85",
      "volume": "36",
      "author": "Puech V.",
      "year": "2000",
      "journal-title": "Mol Microbiol"
    },
    {
      "key": "e_1_2_6_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1997.00758.x"
    },
    {
      "key": "e_1_2_6_28_2",
      "first-page": "547",
      "volume-title": "The Mycobacteria: A Source Book, Part A.",
      "author": "Segal W.",
      "year": "1984"
    },
    {
      "key": "e_1_2_6_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.72.2.132-141.1956"
    },
    {
      "key": "e_1_2_6_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/351456a0"
    },
    {
      "key": "e_1_2_6_31_2",
      "first-page": "20",
      "article-title": "Aerosol infection of mice with mycobacteria using a nose\u2010only exposure device",
      "volume": "2",
      "author": "Tsenova L.",
      "year": "1997",
      "journal-title": "J Am Biol Safety Assoc"
    },
    {
      "key": "e_1_2_6_32_2",
      "first-page": "1530",
      "article-title": "Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester",
      "volume": "42",
      "author": "Tsuchiya S.",
      "year": "1982",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_6_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.23.12828"
    },
    {
      "key": "e_1_2_6_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.15.10041"
    },
    {
      "key": "e_1_2_6_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.1998.01026.x"
    }
  ],
  "container-title": "Molecular Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2958.2000.01882.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2000.01882.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        29
      ]
    ],
    "date-time": "2023-10-29T07:01:27Z",
    "timestamp": 1698562887000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2958.2000.01882.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2000,
        5
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2000,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.2000.01882.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.2000.01882.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2000,
        5
      ]
    ]
  }
}