DOI: 10.1046/j.1365-2958.2000.01740.x. A chromosomally encoded regulator is required for expression of the <i>Yersinia enterocolitica inv</i> gene and for virulence

A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence

10.1046/j.1365-2958.2000.01740.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

The primary invasion factor of Yersinia enterocolitica, invasin, is encoded by inv. inv expression is regulated in response to pH, growth phase and temperature. In vitro, inv is maximally expressed at 26°C, pH 8.0, or 37°C, pH 5.5, in early stationary phase. At 37°C, pH 8.0, inv is weakly expressed. To identify which gene(s) are required for inv regulation, we screened for transposon insertions that decreased expression of an inv–′phoA chromosomal reporter at 26°C. Of 30 000 mutants screened, two were identified that had negligible inv expression in all conditions tested. Both of these independent mutants had an insertion into the same gene, designated rovA (regulator of virulence). RovA has 77% amino acid identity to the Salmonella typhimurium transcriptional regulator SlyA. Complementation with the wild‐type rovA allele restores wild‐type inv expression as monitored by Western blot analysis, tissue culture invasion assay and alkaline phosphatase assay. There is also a significant decrease in invasin levels in bacteria recovered from mice infected with the rovA mutant; therefore, RovA regulates inv expression in vivo as well as in vitro. In the mouse infection model, an inv mutant has a wild‐type LD50, even though the kinetics of infection is changed. In contrast, the rovA mutant has altered kinetics, as well as a 70‐fold increase in the LD50 compared with wild type. Furthermore, because the rovA mutant is attenuated in the mouse model, this suggests that RovA regulates other virulence factors in addition to inv. Analysis of other proposed virulence factors such as Ail, YadA and the Yop proteins shows no regulatory role for RovA. The more severe animal phenotype combined with the lack of impact on known virulence genes aside from inv suggests RovA regulates potentially novel virulence genes of Y. enterocolitica during infection.

Bibliography

Revell, P. A., & Miller, V. L. (2000). A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence. Molecular Microbiology, 35(3), 677â685. Portico.

Authors 2

Paula A. Revell (first)
Virginia L. Miller (additional)

References 28 Referenced 103

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:28 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 3:06 a.m.)
Indexed	1 month, 1 week ago (July 19, 2025, 11:55 p.m.)
Issued	25 years, 7 months ago (Feb. 1, 2000)
Published	25 years, 7 months ago (Feb. 1, 2000)
Published Online	23 years, 4 months ago (April 5, 2002)
Published Print	25 years, 7 months ago (Feb. 1, 2000)

Funders 0

None

BibTeX

@article{Revell_2000, title={A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence}, volume={35}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.2000.01740.x}, DOI={10.1046/j.1365-2958.2000.01740.x}, number={3}, journal={Molecular Microbiology}, publisher={Wiley}, author={Revell, Paula A. and Miller, Virginia L.}, year={2000}, month=feb, pages={677–685} }

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  "abstract": "<jats:p>The primary invasion factor of <jats:italic>Yersinia enterocolitica</jats:italic>, invasin, is encoded by <jats:italic>inv</jats:italic>. <jats:italic>inv</jats:italic> expression is regulated in response to pH, growth phase and temperature. <jats:italic>In vitro</jats:italic>, <jats:italic>inv</jats:italic> is maximally expressed at 26\u00b0C, pH\u20038.0, or 37\u00b0C, pH\u20035.5, in early stationary phase. At 37\u00b0C, pH\u20038.0, <jats:italic>inv</jats:italic> is weakly expressed. To identify which gene(s) are required for <jats:italic>inv</jats:italic> regulation, we screened for transposon insertions that decreased expression of an <jats:italic>inv\u2013\u2032phoA</jats:italic> chromosomal reporter at 26\u00b0C. Of 30\u2003000 mutants screened, two were identified that had negligible <jats:italic>inv</jats:italic> expression in all conditions tested. Both of these independent mutants had an insertion into the same gene, designated <jats:italic>rovA</jats:italic> (<jats:styled-content>r</jats:styled-content>egulator <jats:styled-content>o</jats:styled-content>f <jats:styled-content>v</jats:styled-content>irulence). RovA has 77% amino acid identity to the <jats:italic>Salmonella typhimurium</jats:italic> transcriptional regulator SlyA. Complementation with the wild\u2010type <jats:italic>rovA</jats:italic> allele restores wild\u2010type <jats:italic>inv</jats:italic> expression as monitored by Western blot analysis, tissue culture invasion assay and alkaline phosphatase assay. There is also a significant decrease in invasin levels in bacteria recovered from mice infected with the <jats:italic>rovA</jats:italic> mutant; therefore, RovA regulates <jats:italic>inv</jats:italic> expression <jats:italic>in vivo</jats:italic> as well as <jats:italic>in vitro</jats:italic>. In the mouse infection model, an <jats:italic>inv</jats:italic> mutant has a wild\u2010type LD<jats:sub>50</jats:sub>, even though the kinetics of infection is changed. In contrast, the <jats:italic>rovA</jats:italic> mutant has altered kinetics, as well as a 70\u2010fold increase in the LD<jats:sub>50</jats:sub> compared with wild type. Furthermore, because the <jats:italic>rovA</jats:italic> mutant is attenuated in the mouse model, this suggests that RovA regulates other virulence factors in addition to <jats:italic>inv</jats:italic>. Analysis of other proposed virulence factors such as Ail, YadA and the Yop proteins shows no regulatory role for RovA. The more severe animal phenotype combined with the lack of impact on known virulence genes aside from <jats:italic>inv</jats:italic> suggests RovA regulates potentially novel virulence genes of <jats:italic>Y. enterocolitica</jats:italic> during infection.</jats:p>",
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