DOI: 10.1046/j.1365-2958.1999.01601.x. LipC, a second lipase of <i>Pseudomonas aeruginosa</i>, is LipB and Xcp dependent and is transcriptionally regulated by pilus biogenesis components

LipC, a second lipase of Pseudomonas aeruginosa, is LipB and Xcp dependent and is transcriptionally regulated by pilus biogenesis components

10.1046/j.1365-2958.1999.01601.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

We have isolated cosmids that complement a Pseudomonas aeruginosa export‐impaired mutant by increasing growth on lipid agar, a medium that requires lipase expression and export. These cosmids encode a previously unidentified lipase, LipC, which has high homology to the P. aeruginosa lipA gene product. Like LipA, LipC activity requires the chaperone activity of the lipB gene product and a functional xcp gene cluster for export. However, expression of LipC is barely detectable in a wild‐type background. Transposon insertions that increase lipC promoter activity have been obtained that inactivate two pilus biogenesis genes, pilX and pilY1. This suggests that these proteins either directly or indirectly repress the expression of LipC and may be involved in transducing an extracellular signal that regulates this lipase.

Bibliography

MartÃnez, A., Ostrovsky, P., & Nunn, D. N. (1999). LipC, a second lipase of Pseudomonas aeruginosa, is LipB and Xcp dependent and is transcriptionally regulated by pilus biogenesis components. Molecular Microbiology, 34(2), 317â326. Portico.

Authors 3

Alejandro Martínez (first)
Paula Ostrovsky (additional)
David N. Nunn (additional)

References 24 Referenced 30

10.1111/j.1365-2958.1996.tb02665.x
10.1016/S0021-9258(18)54583-X / J Biol Chem / Nitrate‐inducible formate dehydrogenase in Escherichia coli K‐12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine by Berg B.L. (1991)
10.1007/BF00284205
10.1016/0003-9861(92)90604-U
10.1128/jb.60.1.17-28.1950 / J Bacteriol / Mutants of Escherichia coli requiring vitamin B‐12 by Davis B.D. (1950)
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10.1128/iai.64.8.3252-3258.1996 / Infect Immun / Role of Pseudomonas aeruginosa lipase in inflammatory mediator release from human inflammatory effector cells (platelets, granulocytes, and monocytes) by Konig B. (1996)
Maniatis T. Fritsch E.F. Sambrook J.(1982)Molecular Cloning. A Laboratory Manual.Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.
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Miller J.H.(1972)Experiments in Molecular Genetics.Cold Spring Harbor NY: Cold Spring Harbor Laboratory Press.
10.1111/j.1432-1033.1993.tb18028.x
10.1111/j.1365-2958.1995.mmi_17061057.x
10.1111/j.1574-6968.1992.tb05824.x / FEMS Microbiol Rev / Protein secretion in Pseudomonas aeruginosa by Tommassen J. (1992)
10.1016/0378-1119(91)90221-V
10.1016/S0021-9258(19)84732-4 / J Biol Chem / Evidence for the essential function of 2,4‐dienoyl‐coenzyme A reductase in the beta‐oxidation of unsaturated fatty acids in vivo. Isolation and characterization of an Escherichia coli mutant with a defective 2,4‐dienoyl‐coenzyme A reductase by You S.Y. (1989)

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:27 a.m.)
Deposited	1 year, 10 months ago (Oct. 30, 2023, 11:48 a.m.)
Indexed	3 months, 2 weeks ago (May 22, 2025, 12:11 a.m.)
Issued	25 years, 11 months ago (Oct. 1, 1999)
Published	25 years, 11 months ago (Oct. 1, 1999)
Published Online	23 years, 6 months ago (March 1, 2002)
Published Print	25 years, 11 months ago (Oct. 1, 1999)

Funders 0

None

BibTeX

@article{Mart_nez_1999, title={LipC, a second lipase of Pseudomonas aeruginosa, is LipB and Xcp dependent and is transcriptionally regulated by pilus biogenesis components}, volume={34}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.1999.01601.x}, DOI={10.1046/j.1365-2958.1999.01601.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Martínez, Alejandro and Ostrovsky, Paula and Nunn, David N.}, year={1999}, month=oct, pages={317–326} }

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