DOI: 10.1046/j.1365-2958.1999.01385.x. Characterization of the dual start motif of a class II holin gene

Characterization of the dual start motif of a class II holin gene

10.1046/j.1365-2958.1999.01385.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Holins are small membrane proteins that, at a genetically programmed time in a bacteriophage infective cycle, allow bacteriolytic enzymes, or endolysins, to escape to the periplasm and to attack the cell wall. Most holins fall into two sequence classes, I and II, based on the number of potential transmembrane domains (three for class I and two for class II). The prototype class I holin gene, S λ, has a dual start motif and encodes not only the effector holin, Sλ105, but also an inhibitor, Sλ107, with a Met–Lys … extension at the terminus. The prototype class II holin gene of phage 21, S 21, begins with the motif Met–Lys–Ser–Met … , and a potential RNA secondary structure overlaps the Shine–Dalgarno sequence. Here, we demonstrate that (i) two protein products are elaborated from S 21, S2171 and S2168; (ii) the shorter product is required for lysis; (iii) the longer product, S2171, inhibits S 21 function; and (iv) the Lys‐2 residue is important for the inhibitor function. Moreover, the RNA stem–loop structure is involved in the downregulation of S2171 synthesis. However, our results suggest that, in S 21, different segments of the single consensus Shine–Dalgarno sequence serve the two translational starts. These results show that the dual start motifs of class II holin genes are functionally homologous to those of class I holin genes.

Bibliography

Barenboim, M., Chang, C., Dib Hajj, F., & Young, R. (1999). Characterization of the dual start motif of a class II holin gene. Molecular Microbiology, 32(4), 715â727. Portico.

Authors 4

Maxim Barenboim (first)
Chung‐Yu Chang (additional)
Fadia Dib Hajj (additional)
Ry Young (additional)

References 48 Referenced 50

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Bläsi U. Fraisl P. Chang C.‐Y. Zhang N. Young R.(1999)The C‐terminal sequence of the λ holin constitutes a cytoplasmic regulatory domain.J Bacteriol(in press). (10.1128/JB.181.9.2922-2929.1999)
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{'key': 'e_1_2_6_39_2', 'first-page': '39', 'article-title': 'The λ holin accumulates beyond the lethal triggering concentration under hyper‐expression conditions', 'volume': '7', 'author': 'Smith D.L.', 'year': '1998', 'journal-title': 'Gene Expr'} / Gene Expr / The λ holin accumulates beyond the lethal triggering concentration under hyper‐expression conditions by Smith D.L. (1998)
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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:24 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 3:07 p.m.)
Indexed	1 year, 1 month ago (June 30, 2024, 5:07 a.m.)
Issued	26 years, 3 months ago (May 1, 1999)
Published	26 years, 3 months ago (May 1, 1999)
Published Online	23 years, 2 months ago (June 20, 2002)
Published Print	26 years, 3 months ago (May 1, 1999)

Funders 0

None

BibTeX

@article{Barenboim_1999, title={Characterization of the dual start motif of a class II holin gene}, volume={32}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.1999.01385.x}, DOI={10.1046/j.1365-2958.1999.01385.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Barenboim, Maxim and Chang, Chung‐Yu and Dib Hajj, Fadia and Young, Ry}, year={1999}, month=may, pages={715–727} }

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  "issued": {
    "date-parts": [
      [
        1999,
        5
      ]
    ]
  },
  "references-count": 48,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1999,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2958.1999.01385.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2958.1999.01385.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        1999,
        5
      ]
    ]
  }
}