DOI: 10.1046/j.1365-2958.1999.01270.x. Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu

Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu

10.1046/j.1365-2958.1999.01270.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Gram‐negative bacteria use type III machines to inject toxic proteins into the cytosol of eukaryotic cells. Pathogenic Yersinia species export 14 Yop proteins by the type III pathway and some of these, named effector Yops, are targeted into macrophages, thereby preventing phagocytosis and allowing bacterial replication within lymphoid tissues. Hitherto, YopB/YopD were thought to insert into the plasma membrane of macrophages and to promote the import of effector Yops into the eukaryotic cytosol. We show here that the type III machines of yersiniae secrete three proteins into the extracellular milieu (YopB, YopD and YopR). Although intrabacterial YopD is required for the injection of toxins into eukaryotic cells, secreted YopB, YopD and YopR are dispensable for this process. Nevertheless, YopB, YopD and YopR are essential for the establishment of Yersinia infections in a mouse model system, suggesting that type III secretion machines function to deliver virulence factors into the extracellular milieu also.

Bibliography

Lee, V. T., & Schneewind, O. (1999). Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu. Molecular Microbiology, 31(6), 1619â1629. Portico.

Authors 2

Vincent T. Lee (first)
Olaf Schneewind (additional)

References 47 Referenced 70

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:23 a.m.)
Deposited	1 year, 9 months ago (Oct. 28, 2023, 2:33 p.m.)
Indexed	2 months, 2 weeks ago (June 4, 2025, 6:29 a.m.)
Issued	26 years, 4 months ago (April 1, 1999)
Published	26 years, 4 months ago (April 1, 1999)
Published Online	23 years, 5 months ago (March 1, 2002)
Published Print	26 years, 4 months ago (April 1, 1999)

Funders 0

None

BibTeX

@article{Lee_1999, title={Type III machines of pathogenic yersiniae secrete virulence factors into the extracellular milieu}, volume={31}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.1999.01270.x}, DOI={10.1046/j.1365-2958.1999.01270.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Lee, Vincent T. and Schneewind, Olaf}, year={1999}, month=apr, pages={1619–1629} }

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