DOI: 10.1046/j.1365-2958.1999.01154.x. Role of SycD, the chaperone of the <i>Yersinia</i> Yop translocators YopB and YopD

Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD

10.1046/j.1365-2958.1999.01154.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Extracellular Yersinia adhering at the surface of a eukaryotic cell translocate effector Yops across the plasma membrane of the cell by a mechanism requiring YopD and YopB, the latter probably mediating pore formation. We studied the role of SycD, the intrabacterial chaperone of YopD. By producing GST–YopB hybrid proteins and SycD in Escherichia coli, we observed that SycD also binds specifically to YopB and that this binding reduces the toxicity of GST–YopB in E. coli. By analysis of a series of truncated GST–YopB proteins, we observed that SycD does not bind to a discrete segment of YopB. Using the same approach, we observed that YopD can also bind to YopB. Binding between YopB and YopD occurred even in the presence of SycD, and a complex composed of these three proteins could be immunoprecipitated from the cytoplasm of Yersinia. In a sycD mutant, the intracellular pool of YopB and YopD was greatly reduced unless the lcrV gene was also deleted. As LcrV is known to interact with YopB and YopD and to promote their secretion, we speculate that SycD prevents a premature association between YopB–YopD and LcrV.

Bibliography

Neyt, C., & Cornelis, G. R. (1999). Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD. Molecular Microbiology, 31(1), 143â156. Portico.

Authors 2

Cécile Neyt (first)
Guy R. Cornelis (additional)

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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 6:21 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 11:29 p.m.)
Indexed	1 month ago (July 20, 2025, 12:03 a.m.)
Issued	26 years, 7 months ago (Jan. 1, 1999)
Published	26 years, 7 months ago (Jan. 1, 1999)
Published Online	23 years, 5 months ago (March 1, 2002)
Published Print	26 years, 7 months ago (Jan. 1, 1999)

Funders 0

None

BibTeX

@article{Neyt_1999, title={Role of SycD, the chaperone of the Yersinia Yop translocators YopB and YopD}, volume={31}, ISSN={1365-2958}, url={http://dx.doi.org/10.1046/j.1365-2958.1999.01154.x}, DOI={10.1046/j.1365-2958.1999.01154.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Neyt, Cécile and Cornelis, Guy R.}, year={1999}, month=jan, pages={143–156} }

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