DOI: 10.1046/j.1365-2818.2003.01245.x. High‐pressure freezing in the study of animal pathogens

High‐pressure freezing in the study of animal pathogens

10.1046/j.1365-2818.2003.01245.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SummaryHigh‐pressure freezing is applicable to both morphological and immunocytochemical studies. We are investigating the morphogenesis of foot‐and‐mouth disease virus and African swine fever virus by the use of high‐pressure freezing of infected cells. Foot‐and‐mouth disease virus particles are not detected in sections of conventionally immersion‐fixed infected cells, but when the cells are prepared by high‐pressure freezing, newly formed virions are readily seen throughout the cell. We report two methods for high‐pressure freezing of virally infected cells: first, two sapphire discs frozen ‘face to face’ with a narrow spacer to prevent cell damage and, second, a fibrous filter substrate that can be easily cut into discs to fit into the freezing planchettes. Cells readily adhere to the fibres in vitro, and the complete disc can be rapidly transferred to the planchettes for freezing. Immunolabelling studies of the microneme proteins of the parasite Eimeria tenella indicate that high‐pressure freezing followed by freeze‐substitution in acetone with uranyl acetate allows high‐sensitivity immunolabelling for these proteins.

Bibliography

Monaghan, P., Cook, H., Hawes, P., Simpson, J., & Tomley, F. (2003). Highâpressure freezing in the study of animal pathogens. Journal of Microscopy, 212(1), 62â70. Portico.

Authors 5

P. Monaghan (first)
H. Cook (additional)
P. Hawes (additional)
J. Simpson (additional)
F. Tomley (additional)

References 20 Referenced 31

10.1177/40.5.1349317
{'key': 'e_1_2_6_3_1', 'first-page': '147', 'article-title': 'Characteristics of the poliovirus replication complex', 'volume': '9', 'author': 'Bienz K.', 'year': '1994', 'journal-title': 'Arch. Virol'} / Arch. Virol / Characteristics of the poliovirus replication complex by Bienz K. (1994)
10.1128/jvi.66.5.2740-2747.1992 / J. Virol / Structural and functional‐characterization of the poliovirus replication complex by Bienz K. (1992)
10.1016/0042-6822(83)90531-7
10.1128/jvi.70.12.8382-8390.1996 / J. Virol / Involvement of the endoplasmic reticulum in the assembly and envelopment of African swine fever virus by Cobbold C. (1996)
10.1016/0042-6822(65)90001-2
{'key': 'e_1_2_6_8_1', 'first-page': '341', 'article-title': 'Immuno‐gold‐labeling of Cut‐1, Cut‐2 and cuticlin epitopes in Caenorhabditis elegans and Heterorhabditis Sp processed by high‐pressure freezing and freeze‐substitution', 'volume': '27', 'author': 'Favre R.', 'year': '1995', 'journal-title': 'J. Submicrosc. Cytol. Pathol'} / J. Submicrosc. Cytol. Pathol / Immuno‐gold‐labeling of Cut‐1, Cut‐2 and cuticlin epitopes in Caenorhabditis elegans and Heterorhabditis Sp processed by high‐pressure freezing and freeze‐substitution by Favre R. (1995)
10.1083/jcb.153.3.449
10.1111/j.1365-2818.1994.tb04785.x
10.1046/j.1365-2818.1998.00387.x
{'key': 'e_1_2_6_12_1', 'first-page': '5088', 'article-title': 'An immunocytochemical and ultrastructural‐study of heterogeneity in the human‐breast carcinoma cell‐line Pmc42', 'volume': '45', 'author': 'Monaghan P.', 'year': '1985', 'journal-title': 'Cancer Res'} / Cancer Res / An immunocytochemical and ultrastructural‐study of heterogeneity in the human‐breast carcinoma cell‐line Pmc42 by Monaghan P. (1985)
10.1111/j.1365-2818.1992.tb03253.x
10.1128/JVI.72.3.2373-2387.1998 / J. Virol / African swine fever virus is wrapped by the endoplasmic reticulum by Rouiller I. (1998)
10.1046/j.1365-2818.2001.00787.x
10.1128/JVI.70.10.6576-6588.1996
{'key': 'e_1_2_6_17_1', 'first-page': '1', 'volume-title': 'Biotechnology. Guidelines on Techniques in Coccidiosis Research', 'author': 'Shirley M.W.', 'year': '1995'} / Biotechnology. Guidelines on Techniques in Coccidiosis Research by Shirley M.W. (1995)
10.1016/S0020-7519(01)00255-7
10.1016/S0968-4328(01)00037-3
10.1016/0378-1135(82)90055-4
10.1099/0022-1317-41-1-105

Dates

Type	When
Created	21 years, 10 months ago (Oct. 14, 2003, 6:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 1:20 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 6:37 p.m.)
Issued	21 years, 10 months ago (Oct. 1, 2003)
Published	21 years, 10 months ago (Oct. 1, 2003)
Published Online	21 years, 10 months ago (Oct. 1, 2003)
Published Print	21 years, 10 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Monaghan_2003, title={High‐pressure freezing in the study of animal pathogens}, volume={212}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2003.01245.x}, DOI={10.1046/j.1365-2818.2003.01245.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Monaghan, P. and Cook, H. and Hawes, P. and Simpson, J. and Tomley, F.}, year={2003}, month=oct, pages={62–70} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        8,
        4
      ]
    ],
    "date-time": "2024-08-04T22:37:50Z",
    "timestamp": 1722811070240
  },
  "reference-count": 20,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2003,
            10,
            1
          ]
        ],
        "date-time": "2003-10-01T00:00:00Z",
        "timestamp": 1064966400000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        10
      ]
    ]
  },
  "abstract": "<jats:title>Summary</jats:title><jats:p>High\u2010pressure freezing is applicable to both morphological and immunocytochemical studies. We are investigating the morphogenesis of foot\u2010and\u2010mouth disease virus and African swine fever virus by the use of high\u2010pressure freezing of infected cells. Foot\u2010and\u2010mouth disease virus particles are not detected in sections of conventionally immersion\u2010fixed infected cells, but when the cells are prepared by high\u2010pressure freezing, newly formed virions are readily seen throughout the cell. We report two methods for high\u2010pressure freezing of virally infected cells: first, two sapphire discs frozen \u2018face to face\u2019 with a narrow spacer to prevent cell damage and, second, a fibrous filter substrate that can be easily cut into discs to fit into the freezing planchettes. Cells readily adhere to the fibres <jats:italic>in vitro</jats:italic>, and the complete disc can be rapidly transferred to the planchettes for freezing. Immunolabelling studies of the microneme proteins of the parasite <jats:italic>Eimeria tenella</jats:italic> indicate that high\u2010pressure freezing followed by freeze\u2010substitution in acetone with uranyl acetate allows high\u2010sensitivity immunolabelling for these proteins.</jats:p>",
  "DOI": "10.1046/j.1365-2818.2003.01245.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        10,
        14
      ]
    ],
    "date-time": "2003-10-14T10:34:47Z",
    "timestamp": 1066127687000
  },
  "page": "62-70",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 31,
  "title": "High\u2010pressure freezing in the study of animal pathogens",
  "prefix": "10.1111",
  "volume": "212",
  "author": [
    {
      "given": "P.",
      "family": "Monaghan",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "H.",
      "family": "Cook",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "P.",
      "family": "Hawes",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "J.",
      "family": "Simpson",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "F.",
      "family": "Tomley",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2003,
        10
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/40.5.1349317"
    },
    {
      "key": "e_1_2_6_3_1",
      "first-page": "147",
      "article-title": "Characteristics of the poliovirus replication complex",
      "volume": "9",
      "author": "Bienz K.",
      "year": "1994",
      "journal-title": "Arch. Virol"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "2740",
      "DOI": "10.1128/jvi.66.5.2740-2747.1992",
      "article-title": "Structural and functional\u2010characterization of the poliovirus replication complex",
      "volume": "66",
      "author": "Bienz K.",
      "year": "1992",
      "journal-title": "J. Virol"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(83)90531-7"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "8382",
      "DOI": "10.1128/jvi.70.12.8382-8390.1996",
      "article-title": "Involvement of the endoplasmic reticulum in the assembly and envelopment of African swine fever virus",
      "volume": "70",
      "author": "Cobbold C.",
      "year": "1996",
      "journal-title": "J. Virol"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0042-6822(65)90001-2"
    },
    {
      "key": "e_1_2_6_8_1",
      "first-page": "341",
      "article-title": "Immuno\u2010gold\u2010labeling of Cut\u20101, Cut\u20102 and cuticlin epitopes in Caenorhabditis elegans and Heterorhabditis Sp processed by high\u2010pressure freezing and freeze\u2010substitution",
      "volume": "27",
      "author": "Favre R.",
      "year": "1995",
      "journal-title": "J. Submicrosc. Cytol. Pathol"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.153.3.449"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1994.tb04785.x"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.1998.00387.x"
    },
    {
      "key": "e_1_2_6_12_1",
      "first-page": "5088",
      "article-title": "An immunocytochemical and ultrastructural\u2010study of heterogeneity in the human\u2010breast carcinoma cell\u2010line Pmc42",
      "volume": "45",
      "author": "Monaghan P.",
      "year": "1985",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1992.tb03253.x"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "2373",
      "DOI": "10.1128/JVI.72.3.2373-2387.1998",
      "article-title": "African swine fever virus is wrapped by the endoplasmic reticulum",
      "volume": "72",
      "author": "Rouiller I.",
      "year": "1998",
      "journal-title": "J. Virol"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2818.2001.00787.x"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JVI.70.10.6576-6588.1996"
    },
    {
      "key": "e_1_2_6_17_1",
      "first-page": "1",
      "volume-title": "Biotechnology. Guidelines on Techniques in Coccidiosis Research",
      "author": "Shirley M.W.",
      "year": "1995"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0020-7519(01)00255-7"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-4328(01)00037-3"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1135(82)90055-4"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/0022-1317-41-1-105"
    }
  ],
  "container-title": "Journal of Microscopy",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1365-2818.2003.01245.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2818.2003.01245.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        14
      ]
    ],
    "date-time": "2023-10-14T17:20:12Z",
    "timestamp": 1697304012000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.1365-2818.2003.01245.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2003,
        10
      ]
    ]
  },
  "references-count": 20,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          2003,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.1365-2818.2003.01245.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.1365-2818.2003.01245.x",
  "relation": {},
  "ISSN": [
    "0022-2720",
    "1365-2818"
  ],
  "subject": [],
  "container-title-short": "Journal of Microscopy",
  "published": {
    "date-parts": [
      [
        2003,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2003-10-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}