DOI: 10.1046/j.1365-2818.2003.01244.x. An oscillating cryo‐knife reduces cutting‐induced deformation of vitreous ultrathin sections

An oscillating cryo‐knife reduces cutting‐induced deformation of vitreous ultrathin sections

10.1046/j.1365-2818.2003.01244.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SummaryA new oscillating cryo‐knife for producing uncompressed vitreous sections is introduced. The knife is a modified cryo diamond knife that is driven by a piezo translator. Optimal setting for the oscillation was found to be in the inaudible frequency range of 20–25 kHz. Yeast cells and polystyrene spheres were used as model systems to describe compression in the vitreous sections. We found that compression could be reduced by a factor of about 2 when the knife was oscillating. When the oscillator was turned off, sections were compressed by 40–45%. However, only 15–25% compression was obtained when the knife was oscillating. In some cases completely uncompressed sections of yeast cells were produced. It was also found that the amount of compression depends on the specimen itself and on its embedding medium. With the results shown here, we demonstrate that the oscillating knife can produce high‐quality vitreous sections with minimum cutting artefacts.

Bibliography

AlâAmoudi, A., Dubochet, J., Gnaegi, H., LÃ¼thi, W., & Studer, D. (2003). An oscillating cryoâknife reduces cuttingâinduced deformation of vitreous ultrathin sections. Journal of Microscopy, 212(1), 26â33. Portico.

Authors 5

A. Al‐Amoudi (first)
J. Dubochet (additional)
H. Gnaegi (additional)
W. Lüthi (additional)
D. Studer (additional)

References 18 Referenced 66

10.1038/308032a0
10.1046/j.1365-2818.2002.01051.x
10.1083/jcb.49.3.731
10.1017/S0033583500004297
10.1242/jcs.20.3.687 / J. Cell Sci / Ultracryotomy of biological tissues to preserve membrane structure by Hodson S. (1976)
{'key': 'e_1_2_6_7_1', 'first-page': '147', 'article-title': 'Use of low‐angle diamond knives leads to improved ultrastructural preservation of ultrathin sections', 'volume': '3', 'author': 'Jésior J.‐C.', 'year': '1989', 'journal-title': 'Scanning Microsc. Suppl'} / Scanning Microsc. Suppl / Use of low‐angle diamond knives leads to improved ultrastructural preservation of ultrathin sections by Jésior J.‐C. (1989)
10.1016/0923-2508(91)90035-9
10.1083/jcb.144.6.1135
10.1046/j.1365-2818.1996.1090666.x
10.1007/978-3-642-72815-0_8
10.1083/jcb.48.1.219
10.1016/0968-4328(94)90001-9
10.1111/j.1365-2818.1993.tb03393.x
10.1046/j.1365-2818.1998.00328.x
10.1046/j.1365-2818.2000.00638.x
10.1046/j.1365-2818.2001.00919.x
10.1007/BF00216809
10.1111/j.1365-2818.1995.tb03648.x

Dates

Type	When
Created	21 years, 10 months ago (Oct. 14, 2003, 6:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 1:20 p.m.)
Indexed	4 weeks, 1 day ago (July 22, 2025, 7:13 a.m.)
Issued	21 years, 10 months ago (Oct. 1, 2003)
Published	21 years, 10 months ago (Oct. 1, 2003)
Published Online	21 years, 10 months ago (Oct. 1, 2003)
Published Print	21 years, 10 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Al_Amoudi_2003, title={An oscillating cryo‐knife reduces cutting‐induced deformation of vitreous ultrathin sections}, volume={212}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2003.01244.x}, DOI={10.1046/j.1365-2818.2003.01244.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Al‐Amoudi, A. and Dubochet, J. and Gnaegi, H. and Lüthi, W. and Studer, D.}, year={2003}, month=oct, pages={26–33} }

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