DOI: 10.1046/j.1365-2818.2003.01226.x. A rapid microbiopsy system to improve the preservation of biological samples prior to high‐pressure freezing

A rapid microbiopsy system to improve the preservation of biological samples prior to high‐pressure freezing

10.1046/j.1365-2818.2003.01226.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

SummaryA microbiopsy system for fast excision and transfer of biological specimens from donor to high‐pressure freezer was developed. With a modified, commercially available, Promag 1.2 biopsy gun, tissue samples can be excised with a size small enough (0.6 mm × 1.2 mm × 0.3 mm) to be easily transferred into a newly designed specimen platelet. A self‐made transfer unit allows fast transfer of the specimen from the needle into the specimen platelet. The platelet is then fixed in a commercially available specimen holder of a high‐pressure freezing machine (EM PACT, Leica Microsystems, Vienna, Austria) and frozen therein. The time required by a well‐instructed (but not experienced) person to execute all steps is in the range of half a minute. This period is considered short enough to maintain the excised tissue pieces close to their native state. We show that a range of animal tissues (liver, brain, kidney and muscle) are well preserved. To prove the quality of freezing achieved with the system, we show vitrified ivy leaves high‐pressure frozen in the new specimen platelet.

Bibliography

Vanhecke, D., Graber, W., Herrmann, G., AlâAmoudi, A., Eggli, P., & Studer, D. (2003). A rapid microbiopsy system to improve the preservation of biological samples prior to highâpressure freezing. Journal of Microscopy, 212(1), 3â12. Portico.

Authors 6

D. Vanhecke (first)
W. Graber (additional)
G. Herrmann (additional)
A. Al‐Amoudi (additional)
P. Eggli (additional)
D. Studer (additional)

References 31 Referenced 26

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10.1111/j.1365-2818.1982.tb00379.x
10.1016/0248-4900(91)90086-3
10.1111/j.1365-2818.1994.tb04785.x
10.1046/j.1365-2818.1996.820642.x
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10.1046/j.1365-2818.1998.00387.x
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{'issue': '5', 'key': 'e_1_2_6_15_1', 'first-page': '129', 'article-title': 'Freezing in a propane jet and its application in freeze‐fracturing', 'volume': '36', 'author': 'Müller M.', 'year': '1980', 'journal-title': 'Mikroskopie'} / Mikroskopie / Freezing in a propane jet and its application in freeze‐fracturing by Müller M. (1980)
{'key': 'e_1_2_6_16_1', 'first-page': '131', 'volume-title': 'The Science of Biological Specimen Preparation for Microscopy and Microanalysis', 'author': 'Müller M.', 'year': '1984'} / The Science of Biological Specimen Preparation for Microscopy and Microanalysis by Müller M. (1984)
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{'key': 'e_1_2_6_26_1', 'first-page': '253', 'article-title': 'High pressure freezing comes of age', 'volume': '3', 'author': 'Studer D.', 'year': '1989', 'journal-title': 'Scanning Microsc. Suppl'} / Scanning Microsc. Suppl / High pressure freezing comes of age by Studer D. (1989)
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Dates

Type	When
Created	21 years, 10 months ago (Oct. 14, 2003, 6:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 1:19 p.m.)
Indexed	1 year, 3 months ago (May 9, 2024, 12:37 a.m.)
Issued	21 years, 10 months ago (Oct. 1, 2003)
Published	21 years, 10 months ago (Oct. 1, 2003)
Published Online	21 years, 10 months ago (Oct. 1, 2003)
Published Print	21 years, 10 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Vanhecke_2003, title={A rapid microbiopsy system to improve the preservation of biological samples prior to high‐pressure freezing}, volume={212}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2003.01226.x}, DOI={10.1046/j.1365-2818.2003.01226.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Vanhecke, D. and Graber, W. and Herrmann, G. and Al‐Amoudi, A. and Eggli, P. and Studer, D.}, year={2003}, month=oct, pages={3–12} }

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