DOI: 10.1046/j.1365-2818.2001.00784.x. Observation of human dentine by focused ion beam and energy‐filtering transmission electron microscopy

Observation of human dentine by focused ion beam and energy‐filtering transmission electron microscopy

10.1046/j.1365-2818.2001.00784.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

Molar dentine was sliced into 100 nm ultrathin sections, by means of a focused ion beam, for observation by energy‐filtering transmission electron microscopy (EFTEM). Within the matrix, crystals approximately 10 nm wide and 50–100 nm long were clearly observed. When carbon and calcium were mapped in electron spectroscopic images by EFTEM, carbon failed to localize in crystals. However, it was found in other regions, especially those adjacent to crystals. Because carbon localizations were thought to reflect the presence of organic components, carbon concentration in regions near crystals suggested the interaction of crystals and organics, leading to organic control of apatite formation and growth. Ca was present in almost all regions. The majority of Ca localizing in regions other than crystals may be bound to organic substances present in dentine matrix. These substances are thought to both accumulate Ca and act as reservoirs for crystallization of apatite in dentine.

Bibliography

Hoshi, K., Ejiri, S., Probst, W., Seybold, V., Kamino, T., Yaguchi, T., Yamahira, N., & Ozawa, H. (2001). Observation of human dentine by focused ion beam and energyâfiltering transmission electron microscopy. Journal of Microscopy, 201(1), 44â49. Portico.

Authors 8

K. Hoshi (first)
S. Ejiri (additional)
W. Probst (additional)
V. Seybold (additional)
T. Kamino (additional)
T. Yaguchi (additional)
N. Yamahira (additional)
H. Ozawa (additional)

References 23 Referenced 25

10.1007/BF02555795
10.1073/pnas.80.5.1322
10.1083/jcb.98.3.911
10.1016/S0889-1605(88)80932-7
10.1007/BF02561410
10.1111/j.1365-2818.1991.tb03126.x
10.1007/BF00218798
10.1007/s004180050214
{'key': 'e_1_2_7_10_1', 'first-page': '107', 'article-title': 'Extracellular matrix proteins of dentine', 'volume': '205', 'author': 'Butler W.T.', 'year': '1997', 'journal-title': 'Ciba Foundation Symposium'} / Ciba Foundation Symposium / Extracellular matrix proteins of dentine by Butler W.T. (1997)
{'key': 'e_1_2_7_11_1', 'first-page': '101', 'volume-title': 'Oral Histology: Development, Structure, and Function.', 'author': 'Ten Cate A.R.', 'year': '1985'} / Oral Histology: Development, Structure, and Function. by Ten Cate A.R. (1985)
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{'key': 'e_1_2_7_13_1', 'first-page': '145', 'article-title': 'A carbon image of a cryosection of Escherichia coli by electron spectroscopic imaging (ESI)', 'volume': '34', 'author': 'Futaesaku Y.', 'year': '1999', 'journal-title': 'J. Electron Microsc'} / J. Electron Microsc / A carbon image of a cryosection of Escherichia coli by electron spectroscopic imaging (ESI) by Futaesaku Y. (1999)
{'key': 'e_1_2_7_14_1', 'first-page': '234', 'article-title': 'High‐resolution electron microscopy of human enamel reactions prepared with focused ion beam system', 'volume': '20', 'author': 'Hayashi Y.', 'year': '1998', 'journal-title': 'Proc. SCANNING'} / Proc. SCANNING / High‐resolution electron microscopy of human enamel reactions prepared with focused ion beam system by Hayashi Y. (1998)
{'key': 'e_1_2_7_15_1', 'first-page': '263', 'article-title': 'Energy‐filtering transmission electron microscopy of Carl Zeiss', 'volume': '32', 'author': 'Hoshi K.', 'year': '2000', 'journal-title': 'SAIBO'} / SAIBO / Energy‐filtering transmission electron microscopy of Carl Zeiss by Hoshi K. (2000)
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{'key': 'e_1_2_7_24_1', 'first-page': ')', 'volume-title': 'Biological Mineralization', 'author': 'Weatherell J.A.', 'year': '1973'} / Biological Mineralization by Weatherell J.A. (1973)

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:31 a.m.)
Deposited	1 year, 10 months ago (Oct. 18, 2023, 5:16 a.m.)
Indexed	1 year ago (July 28, 2024, 5:13 p.m.)
Issued	24 years, 7 months ago (Jan. 1, 2001)
Published	24 years, 7 months ago (Jan. 1, 2001)
Published Online	23 years, 8 months ago (Dec. 21, 2001)
Published Print	24 years, 7 months ago (Jan. 1, 2001)

Funders 0

None

BibTeX

@article{Hoshi_2001, title={Observation of human dentine by focused ion beam and energy‐filtering transmission electron microscopy}, volume={201}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2001.00784.x}, DOI={10.1046/j.1365-2818.2001.00784.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Hoshi, K. and Ejiri, S. and Probst, W. and Seybold, V. and Kamino, T. and Yaguchi, T. and Yamahira, N. and Ozawa, H.}, year={2001}, month=jan, pages={44–49} }

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