DOI: 10.1046/j.1365-2818.2000.00710.x. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy

Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy

10.1046/j.1365-2818.2000.00710.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

Lateral resolution that exceeds the classical diffraction limit by a factor of two is achieved by using spatially structured illumination in a wide‐field fluorescence microscope. The sample is illuminated with a series of excitation light patterns, which cause normally inaccessible high‐resolution information to be encoded into the observed image. The recorded images are linearly processed to extract the new information and produce a reconstruction with twice the normal resolution. Unlike confocal microscopy, the resolution improvement is achieved with no need to discard any of the emission light. The method produces images of strikingly increased clarity compared to both conventional and confocal microscopes.

Bibliography

Gustafsson, M. G. L. (2000). Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. Journal of Microscopy, 198(2), 82â87. Portico.

Authors 1

M. G. L. Gustafsson (first)

References 17 Referenced 3,169

10.1007/BF02956173
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Gustafsson M.G.L. Agard D.A. Sedat J.W.(1997) US patent 5671085 cols. 23–25.
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Minsky M.(1961) US patent 3013467.
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Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:30 a.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 5:36 a.m.)
Indexed	5 hours, 20 minutes ago (Sept. 2, 2025, 6:35 a.m.)
Issued	25 years, 4 months ago (May 1, 2000)
Published	25 years, 4 months ago (May 1, 2000)
Published Online	23 years, 8 months ago (Dec. 24, 2001)
Published Print	25 years, 4 months ago (May 1, 2000)

Funders 0

None

BibTeX

@article{Gustafsson_2000, title={Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy: SHORT COMMUNICATION}, volume={198}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2000.00710.x}, DOI={10.1046/j.1365-2818.2000.00710.x}, number={2}, journal={Journal of Microscopy}, publisher={Wiley}, author={Gustafsson, M. G. L.}, year={2000}, month=may, pages={82–87} }

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