DOI: 10.1046/j.1365-2818.2000.00679.x. The reliability of cryoSEM for the observation and quantification of xylem embolisms and quantitative analysis of xylem sap <i>in situ</i>

The reliability of cryoSEM for the observation and quantification of xylem embolisms and quantitative analysis of xylem sap in situ

10.1046/j.1365-2818.2000.00679.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

The reliability of cryoSEM for visualizing gas embolisms in xylem vessels of intact, functioning roots is examined and discussed. The possibility that these embolisms form as a result of freezing water columns under tension is discounted by a double‐freeze experiment. Two regions of the same root, one frozen under tension, the other isolated from the tension by the first freeze, had the same percentage of embolisms, as did also long pieces of root frozen simultaneously along their length. The reliability of energy‐dispersive X‐ray analysis to measure xylem sap concentration in situ in frozen tissue was established by measurement of KCl standard solution frozen on stubs, and within xylem vessels. Solute heterogeneity within the vessels varied with freezing procedure; deep‐freeze > LN2 > cryopliers > liquid ethane, but only the deep‐freeze method gave unsatisfactory estimates of concentration for the standard solution. It is concluded that cryoanalytical SEM is useful for direct observation of gas and liquid‐filled compartments, and for solute analyses at depth within intact plant organs.

Bibliography

Mccully, Shane, Baker, Huang, Ling, & Canny. (2000). The reliability of cryoSEM for the observation and quantification of xylem embolisms and quantitative analysis of xylem sap in situ. Journal of Microscopy, 198(1), 24â33. Portico.

Authors 6

Mccully (first)
Shane (additional)
Baker (additional)
Huang (additional)
Ling (additional)
Canny (additional)

References 33 Referenced 52

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Haberlandt G.(1914)Physiological Plant Anatomy. Macmillan London. (10.5962/bhl.title.28492)
Hess F.D.(1980) Influence of specimen topography on microanalysis.X‐Ray Microanalysis in Biology(ed. by M. A. Hayat) pp.241262. University Park Press Baltimore. (10.1007/978-1-349-06174-7_6)
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{'issue': '2', 'key': 'e_1_2_6_17_2', 'first-page': '327', 'article-title': 'Simultaneous use of EDS, windowless EDS, BE and SE detectors and digital real‐time line‐scanning for the X‐ray micro‐analysis of frozen‐hydrated biological specimens', 'volume': '1981', 'author': 'Marshall A.T.', 'year': '1981', 'journal-title': 'Scanning Electron Microsc'} / Scanning Electron Microsc / Simultaneous use of EDS, windowless EDS, BE and SE detectors and digital real‐time line‐scanning for the X‐ray micro‐analysis of frozen‐hydrated biological specimens by Marshall A.T. (1981)
{'issue': '1', 'key': 'e_1_2_6_18_2', 'first-page': '243', 'article-title': 'Application of φ(ρZ) curves and a windowless detector to the quantitative X‐ray microanalysis of frozen‐hydrated bulk biological specimens', 'volume': '1982', 'author': 'Marshall A.T.', 'year': '1982', 'journal-title': 'Scanning Electron Microsc'} / Scanning Electron Microsc / Application of φ(ρZ) curves and a windowless detector to the quantitative X‐ray microanalysis of frozen‐hydrated bulk biological specimens by Marshall A.T. (1982)
Marshall A.T.(1987) Scanning electron microscopy and X‐ray microanalysis of frozen‐hydrated bulk samples.Cryotechniques in Biological Electron Microscopy(ed. by R. A. Steinbrecht and K. Zeirold) pp.240257. Springer Verlag Berlin. (10.1007/978-3-642-72815-0_13)
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10.1146/annurev.pp.40.060189.000315

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:30 a.m.)
Deposited	1 year, 9 months ago (Oct. 29, 2023, 11:01 a.m.)
Indexed	1 year, 9 months ago (Nov. 19, 2023, 7:40 a.m.)
Issued	25 years, 4 months ago (April 1, 2000)
Published	25 years, 4 months ago (April 1, 2000)
Published Online	23 years, 8 months ago (Dec. 24, 2001)
Published Print	25 years, 4 months ago (April 1, 2000)

Funders 0

None

BibTeX

@article{Mccully_2000, title={The reliability of cryoSEM for the observation and quantification of xylem embolisms and quantitative analysis of xylem sap in situ}, volume={198}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2000.00679.x}, DOI={10.1046/j.1365-2818.2000.00679.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Mccully and Shane and Baker and Huang and Ling and Canny}, year={2000}, month=apr, pages={24–33} }

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