DOI: 10.1046/j.1365-2818.2000.00638.x. Minimal compression of ultrathin sections with use of an oscillating diamond knife

Minimal compression of ultrathin sections with use of an oscillating diamond knife

10.1046/j.1365-2818.2000.00638.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

With the aim to minimize compression artefacts in ultrathin sections, coincident with the stroke direction, we have invented an oscillating diamond knife. Results and theoretical considerations explaining its function are discussed. During conventional ultrathin sectioning the resultant compression is in the order of 20–35% of section height. This holds true for sections of samples embedded into Lowicryl HM20 and of the polymer polystyrene, cut with a 45° diamond knife and floated on water. The oscillating knife reduces this compression almost completely. It consists of a diamond knife on which a low voltage piezoelectric translator (piezo) is mounted, which oscillates when the piezo is driven by an alternating voltage source. No additional cutting artefacts were observed in the micrographs when they were compared with sections produced without oscillating the knife.

Bibliography

Studer, & Gnaegi. (2000). Minimal compression of ultrathin sections with use of an oscillating diamond knife. Journal of Microscopy, 197(1), 94â100. Portico.

Authors 2

Studer (first)
Gnaegi (additional)

References 7 Referenced 72

10.1111/j.1365-2818.1985.tb02660.x
{'key': 'e_1_2_7_3_2', 'first-page': '147', 'article-title': 'Use of low‐angle diamond knives leads to improved ultrastructural preservation of ultrathin sections', 'volume': '3', 'author': 'Jésior J.C.', 'year': '1989', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. / Use of low‐angle diamond knives leads to improved ultrastructural preservation of ultrathin sections by Jésior J.C. (1989)
{'key': 'e_1_2_7_4_2', 'first-page': '245', 'article-title': 'Bändern mit schrägem Messer', 'volume': '2', 'author': 'Olbrich E.', 'year': '1947', 'journal-title': 'Mikroskopie (Wien)'} / Mikroskopie (Wien) / Bändern mit schrägem Messer by Olbrich E. (1947)
10.1083/jcb.17.1.208
10.1016/0968-4328(94)90001-9
Sitte H.&Neumann K.(1983) Ultramikrotome und apparative Hilfsmittel für die Ultramikrotomie.Methodensammlung der Elektronenmikroskopie 1.1.2(ed. by G. Schimmel and W. Vogell) pp.172175. Wissenschaftliche Verlagsgesellschaft Stuttgart.
10.1177/18.8.590

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:29 a.m.)
Deposited	1 year, 9 months ago (Oct. 29, 2023, 12:26 p.m.)
Indexed	1 month, 3 weeks ago (July 3, 2025, 5:44 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 24, 2001)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Studer_2000, title={Minimal compression of ultrathin sections with use of an oscillating diamond knife}, volume={197}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2000.00638.x}, DOI={10.1046/j.1365-2818.2000.00638.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Studer and Gnaegi}, year={2000}, month=jan, pages={94–100} }

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