DOI: 10.1046/j.1365-2818.2000.00630.x. Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: I. Instrumentation, imaging and spectroscopy

Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: I. Instrumentation, imaging and spectroscopy

10.1046/j.1365-2818.2000.00630.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

We have developed a cryo scanning transmission X‐ray microscope which uses soft X‐rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 μm at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 1010 Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography ( Wang et al. (2000 ) Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: II. Tomography. J. Microsc. 197, 80–93) or spectroscopic analysis.

Bibliography

Maser, Osanna, Wang, Jacobsen, Kirz, Spector, Winn, & Tennant. (2000). Soft Xâray microscopy with a cryo scanning transmission Xâray microscope: I. Instrumentation, imaging and spectroscopy. Journal of Microscopy, 197(1), 68â79. Portico.

Authors 8

Maser (first)
Osanna (additional)
Wang (additional)
Jacobsen (additional)
Kirz (additional)
Spector (additional)
Winn (additional)
Tennant (additional)

References 0 Referenced 102

None

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:29 a.m.)
Deposited	1 year, 9 months ago (Oct. 29, 2023, 12:25 p.m.)
Indexed	3 days, 7 hours ago (Aug. 23, 2025, 12:58 a.m.)
Issued	25 years, 7 months ago (Jan. 1, 2000)
Published	25 years, 7 months ago (Jan. 1, 2000)
Published Online	23 years, 8 months ago (Dec. 24, 2001)
Published Print	25 years, 7 months ago (Jan. 1, 2000)

Funders 0

None

BibTeX

@article{Maser_2000, title={Soft X‐ray microscopy with a cryo scanning transmission X‐ray microscope: I. Instrumentation, imaging and spectroscopy}, volume={197}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.2000.00630.x}, DOI={10.1046/j.1365-2818.2000.00630.x}, number={1}, journal={Journal of Microscopy}, publisher={Wiley}, author={Maser and Osanna and Wang and Jacobsen and Kirz and Spector and Winn and Tennant}, year={2000}, month=jan, pages={68–79} }

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