DOI: 10.1046/j.1365-2818.1999.00595.x. Biological ultrastructure as revealed by high resolution cryo‐SEM of block faces after cryo‐sectioning

Biological ultrastructure as revealed by high resolution cryo‐SEM of block faces after cryo‐sectioning

10.1046/j.1365-2818.1999.00595.x

Crossref journal-article

Wiley

Journal of Microscopy (311)

Abstract

Ultrastructural information was obtained by imaging the block face of high‐pressure‐frozen cryo‐sectioned biological samples in a high‐resolution cryo‐SEM. Cryo‐sectioning leads to a well‐defined flat artificial surface in contrast to cryo‐fracturing. Typical artefacts of cryo‐sections such as compression and crevasses were not visible on the block face. The ultrastructural features known from resin sections and from freeze‐fractures could also be found on the block faces. The cytoplasms show particles of different size which most likely represent proteins. The effects of radiation damage could be reduced considerably by applying the double layer coating technique and backscattered electron imaging.High quality cryo‐sections are only obtained from vitrified material. Reasonably flat block faces were, however, also obtained from adequately frozen microcrystalline samples, thereby facilitating ultrastructural studies in the frozen hydrated state.

Bibliography

Walther, & MÃ¼ller. (1999). Biological ultrastructure as revealed by high resolution cryoâSEM of block faces after cryoâsectioning. Journal of Microscopy, 196(3), 279â287. Portico.

Authors 2

Walther (first)
Müller (additional)

References 34 Referenced 72

10.1002/sca.4950140302
10.1002/jemt.1070320509
10.1073/pnas.55.5.1048
10.1002/jemt.1070280108 / Microsc. Res. Techn. / Planing frozen hydrated plant specimens for SEM observation and EDX microanalysis by Cheng X. (1994)
Echlin P.(1971) The examination of biological material at low temperatures.Scanning Electron Microscopy 1971/1(ed. by O. Johari) pp.225232. ITTRI Chicago.
Echlin P.(1992)Low‐Temperature Microscopy and Analysis. Plenum Press New York. (10.1007/978-1-4899-2302-8)
10.1046/j.1365-2818.1998.00323.x
{'issue': '4', 'key': 'e_1_2_8_9_2', 'first-page': '43', 'article-title': 'The ultrastructure of cryo‐sections and intact vitrified cells\u2003−\u2003the effects of cryoprotectants and acceleration voltage on beam induced bubbling', 'volume': '5', 'author': 'Frederik P.M.', 'year': '1991', 'journal-title': 'Scanning Microsc. Suppl.'} / Scanning Microsc. Suppl. / The ultrastructure of cryo‐sections and intact vitrified cells − the effects of cryoprotectants and acceleration voltage on beam induced bubbling by Frederik P.M. (1991)
{'key': 'e_1_2_8_10_2', 'first-page': '343', 'article-title': 'Progress in scanning electron microscopy of frozen‐hydrated biological specimens', 'volume': '7', 'author': 'Hermann R.', 'year': '1993', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. / Progress in scanning electron microscopy of frozen‐hydrated biological specimens by Hermann R. (1993)
{'key': 'e_1_2_8_11_2', 'first-page': '1215', 'article-title': 'Double‐axis rotary shadowing for high‐resolution scanning electron microscopy', 'volume': '2', 'author': 'Hermann R.', 'year': '1988', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. / Double‐axis rotary shadowing for high‐resolution scanning electron microscopy by Hermann R. (1988)
10.1083/jcb.82.1.150
Huang C.X. Ling L.E.C. McCully M.E. Canny M.J.(1996) Cryo‐analytical microscopy: Multiple applications for plant structure and physiology.Proceedings of the 54th Annual Meeting of the Microscopical Society of America 1996(ed. by G. W. Bailey J. M. Corbett R. V. W. Dimlich J. R. Michael and N. J. Zaluzec) p.76. San Francisco Press San Francisco. (10.1017/S0424820100162843)
10.1083/jcb.72.1.47
10.1111/j.1365-2818.1983.tb04225.x
10.1111/j.1365-2818.1992.tb01506.x
10.1111/j.1365-2818.1991.tb03155.x
10.1083/jcb.17.3.609
{'key': 'e_1_2_8_19_2', 'first-page': '33', 'article-title': 'Snap‐freezing under high pressure: a new fixation technique for freeze‐etching', 'volume': '2', 'author': 'Moor H.', 'year': '1968', 'journal-title': 'Proc. 4th European Reg. Conf. Electron Microscopy'} / Proc. 4th European Reg. Conf. Electron Microscopy / Snap‐freezing under high pressure: a new fixation technique for freeze‐etching by Moor H. (1968)
Müller M.&Moor H.(1984) Cryo‐fixation of suspensions and tissues by propane jet freezing and high‐pressure freezing.Proc. 42nd Ann. Meet. Electron Microsc. Soc. Am. (ed. by G. W. Bailey) pp.69. San Francisco Press San Francisco. (10.1017/S0424820100110477)
{'key': 'e_1_2_8_21_2', 'first-page': '901', 'article-title': 'Development of an ultra high resolution scanning electron microscope by means of a field‐emission source and in‐lens systerm', 'volume': '1', 'author': 'Nagatani T.', 'year': '1987', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. / Development of an ultra high resolution scanning electron microscope by means of a field‐emission source and in‐lens systerm by Nagatani T. (1987)
{'key': 'e_1_2_8_22_2', 'first-page': '1359', 'article-title': 'Conditions required for high quality high magnification images in secondary electron‐I scanning electron microscopy', 'author': 'Peters K.‐R.', 'year': '1982', 'journal-title': 'Scanning Electron Microsc.'} / Scanning Electron Microsc. / Conditions required for high quality high magnification images in secondary electron‐I scanning electron microscopy by Peters K.‐R. (1982)
10.1111/j.1365-2818.1986.tb02734.x
10.1046/j.1365-2818.1998.00389.x
{'key': 'e_1_2_8_25_2', 'first-page': '305', 'article-title': 'Freeze‐fracture studies on barley plastid membranes. VI. Location of the P700‐chlorophyll a‐protein 1', 'volume': '31', 'author': 'Simpson D.J.', 'year': '1983', 'journal-title': 'Eur J. Cell Biol.'} / Eur J. Cell Biol. / Freeze‐fracture studies on barley plastid membranes. VI. Location of the P700‐chlorophyll a‐protein 1 by Simpson D.J. (1983)
10.1083/jcb.3.1.45
{'issue': '3', 'key': 'e_1_2_8_27_2', 'first-page': '253', 'article-title': 'High pressure freezing comes of age', 'volume': '3', 'author': 'Studer D.', 'year': '1989', 'journal-title': 'Scanning Microsc'} / Scanning Microsc / High pressure freezing comes of age by Studer D. (1989)
10.1002/sca.4950130108
{'key': 'e_1_2_8_29_2', 'first-page': '301', 'article-title': 'Backscattered electron imaging for high resolution surface SEM with a new type YAG‐detector', 'volume': '5', 'author': 'Walther P.', 'year': '1991', 'journal-title': 'Scanning Microsc.'} / Scanning Microsc. / Backscattered electron imaging for high resolution surface SEM with a new type YAG‐detector by Walther P. (1991)
10.1002/sca.4950190501
10.1111/j.1365-2818.1995.tb03635.x
10.1006/jmbi.1996.0686
Wepf R. Aebi U. Bremer A. Haider M. Tittmann P. Zach J. Gross H.(1994) High resolution SEM of biological macromolecular complexes.Proc. 52nd Ann. Meeting Microsc. Soc. Am. (ed. by G. W. Bailey and A. J. Garratt‐Reed) pp.10261027. San Francisco Press San Francisco. (10.1017/S0424820100172863)
10.1016/0304-3991(82)90186-3
Zierold K.(1986) Preparation of cryosections for biological microanalysis.The Science of Biological Specimen Preparation 1985(ed. by M. Müller R. P. Becker A. Boyde and J. J. Wolosewick) pp.119127. SEM Inc. AMF O'Hare Chicago.

Dates

Type	When
Created	22 years, 5 months ago (March 12, 2003, 1:28 a.m.)
Deposited	1 year, 9 months ago (Oct. 28, 2023, 4:46 p.m.)
Indexed	11 months, 1 week ago (Sept. 10, 2024, 8:37 a.m.)
Issued	25 years, 8 months ago (Dec. 1, 1999)
Published	25 years, 8 months ago (Dec. 1, 1999)
Published Online	23 years, 7 months ago (Dec. 24, 2001)
Published Print	25 years, 8 months ago (Dec. 1, 1999)

Funders 0

None

BibTeX

@article{Walther_1999, title={Biological ultrastructure as revealed by high resolution cryo‐SEM of block faces after cryo‐sectioning}, volume={196}, ISSN={1365-2818}, url={http://dx.doi.org/10.1046/j.1365-2818.1999.00595.x}, DOI={10.1046/j.1365-2818.1999.00595.x}, number={3}, journal={Journal of Microscopy}, publisher={Wiley}, author={Walther and Müller}, year={1999}, month=dec, pages={279–287} }

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