DOI: 10.1046/j.1365-2567.2002.01513.x. Cross‐presentation of cell‐associated antigens by CD8α<sup>+</sup> dendritic cells is attributable to their ability to internalize dead cells

Cross‐presentation of cell‐associated antigens by CD8α+ dendritic cells is attributable to their ability to internalize dead cells

10.1046/j.1365-2567.2002.01513.x

Crossref journal-article

Wiley

Immunology (311)

Abstract

SummaryIn the mouse, cross‐presentation is an exclusive property of the CD8α+ subset of dendritic cells (DC) but the basis for this selectivity remains unclear. Here we report that splenic CD8α+ DC are much superior to other DC subsets in internalizing dying cells in vitro. In contrast, CD8α+, CD8α– CD4+ and CD8α– CD4– DC subsets phagocytose bacteria or latex beads to a similar extent. Although CD8α+ DC are better than CD4+ DC at presenting ovalbumin (OVA)‐loaded splenocytes to naïve OT‐I T lymphocytes, CD4+ DC are better at presenting OVA‐expressing Escherichia coli to the same T cells. In both cases, presentation is abrogated by lactacystin. These results show that both splenic CD8α+ and CD8α– DC can present exogenous antigens on major histocompatibility complex (MHC) class I via a proteasome‐dependent pathway and suggest that the specialized cross‐presenting function of CD8α+ DC is a result of their ability to endocytose dying cells rather than a unique pathway for handling endosomal contents.

Bibliography

Schulz, O., & Reis e Sousa, C. (2002). Crossâpresentation of cellâassociated antigens by CD8Î±+ dendritic cells is attributable to their ability to internalize dead cells. Immunology, 107(2), 183â189. Portico.

Authors 2

Oliver Schulz (first)
Caetano Reis e Sousa (additional)

References 28 Referenced 170

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 9:31 p.m.)
Deposited	1 year, 10 months ago (Oct. 17, 2023, 4:05 a.m.)
Indexed	3 days, 17 hours ago (Aug. 30, 2025, 12:43 p.m.)
Issued	22 years, 11 months ago (Oct. 1, 2002)
Published	22 years, 11 months ago (Oct. 1, 2002)
Published Online	22 years, 10 months ago (Oct. 16, 2002)
Published Print	22 years, 11 months ago (Oct. 1, 2002)

Funders 0

None

BibTeX

@article{Schulz_2002, title={Cross‐presentation of cell‐associated antigens by CD8α+ dendritic cells is attributable to their ability to internalize dead cells}, volume={107}, ISSN={1365-2567}, url={http://dx.doi.org/10.1046/j.1365-2567.2002.01513.x}, DOI={10.1046/j.1365-2567.2002.01513.x}, number={2}, journal={Immunology}, publisher={Wiley}, author={Schulz, Oliver and Reis e Sousa, Caetano}, year={2002}, month=oct, pages={183–189} }

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