DOI: 10.1046/j.1365-2567.2002.01430.x. Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells

10.1046/j.1365-2567.2002.01430.x

Crossref journal-article

Wiley

Immunology (311)

Abstract

SummaryHuman β‐defensins are broad‐spectrum antimicrobial peptides known to be produced by epithelial cells. It was recently shown that β‐defensins also display chemotactic activity for dendritic cells (DC) and T cells, and thus may serve to link innate and adaptive immunity. The aim of the present study was to explore expression of mRNA for these peptides in mononuclear phagocytes and DC. The results revealed that monocytes, monocyte‐derived‐macrophages (MDM), and monocyte‐derived‐dendritic cells (DC) all express human‐β‐defensin‐1 (hBD‐1) mRNA. hBD‐1 mRNA expression by monocytes and MDM was increased after activation with interferon‐γ (IFN‐γ) and/or lipopolysaccharide (LPS) in a dose‐ and time‐dependent fashion. Alveolar macrophages showed an intense hBD‐1 expression, which could not be further increased. Expression of hBD‐1 mRNA by immature DC was low, and increased considerably after maturation. Monocytes, MDM, alveolar macrophages and DC showed a limited expression of human β‐defensin‐2 (hBD‐2) mRNA, which could only be increased in monocytes and alveolar macrophages by IFN‐γ and/or LPS in a dose‐ and time‐dependent fashion. Immunocytochemical stainings demonstrated the expression of hBD‐2 peptide by freshly isolated blood monocytes and alveolar macrophages in cytospin preparations.

Bibliography

Duits, L. A., Ravensbergen, B., Rademaker, M., Hiemstra, P. S., & Nibbering, P. H. (2002). Expression of Î²âdefensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells. Immunology, 106(4), 517â525. Portico.

Authors 5

Louise A. Duits (first)
Bep Ravensbergen (additional)
Mirjam Rademaker (additional)
Pieter S. Hiemstra (additional)
Peter H. Nibbering (additional)

References 48 Referenced 198

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Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 9:31 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 5:35 p.m.)
Indexed	2 months, 1 week ago (June 15, 2025, 7:01 a.m.)
Issued	23 years ago (Aug. 1, 2002)
Published	23 years ago (Aug. 1, 2002)
Published Online	23 years ago (Aug. 1, 2002)
Published Print	23 years ago (Aug. 1, 2002)

Funders 0

None

BibTeX

@article{Duits_2002, title={Expression of β‐defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells}, volume={106}, ISSN={1365-2567}, url={http://dx.doi.org/10.1046/j.1365-2567.2002.01430.x}, DOI={10.1046/j.1365-2567.2002.01430.x}, number={4}, journal={Immunology}, publisher={Wiley}, author={Duits, Louise A. and Ravensbergen, Bep and Rademaker, Mirjam and Hiemstra, Pieter S. and Nibbering, Peter H.}, year={2002}, month=aug, pages={517–525} }

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