DOI: 10.1046/j.1365-2567.2000.00979.x. The effects of DNA containing CpG motif on dendritic cells

The effects of DNA containing CpG motif on dendritic cells

10.1046/j.1365-2567.2000.00979.x

Crossref journal-article

Wiley

Immunology (311)

Abstract

SummaryDendritic cells (DC) are specialized antigen‐presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co‐stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up‐regulate co‐stimulatory molecules, induce T‐cell proliferative responses and secrete interleukin‐12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T‐cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte‐derived human DC in vitro, but lipopolysaccharide‐treated monocyte‐derived DC showed enhanced functional activity and up‐regulated co‐stimulatory molecules.

Bibliography

Behboudi, S., Chao, D., Klenerman, P., & Austyn, J. (2000). The effects of DNA containing CpG motif on dendritic cells. Immunology, 99(3), 361â366. Portico.

Authors 4

S. Behboudi (first)
D. Chao (additional)
P. Klenerman (additional)
J. Austyn (additional)

References 24 Referenced 56

10.1038/374546a0
10.4049/jimmunol.157.5.1840 / J Immunol / Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA by Ballas Z.K. (1996)
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10.1002/(SICI)1521-4141(199806)28:06<2045::AID-IMMU2045>3.0.CO;2-8 / Eur J Immunol / Bacterial DNA immunostimulatory CpG oligonucleotides maturation and activation of murine dendritic cells by Sparwasser T. (1998)
10.4049/jimmunol.161.6.3042 / J Immunol / Activation of cutaneous dendritic cells by CpG‐containing oligodeoxynucleotides: a role for dendritic cells in the augmentation of Th1 responses by immunostimulatory DNA by Jakob T. (1998)
10.1002/eji.1830271242
10.1073/pnas.93.7.2879
10.4049/jimmunol.157.12.5394 / J Immunol / Rapid immune activation by CpG motifs in bacterial DNA. Systemic induction of IL‐6 transcription through an antioxidant‐sensitive pathway by Yi A.K. (1996)
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10.4049/jimmunol.158.8.3635 / J Immunol / Contribution of CpG motifs to the immunogenicity of DNA vaccines by Klinman D.M. (1997)
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10.1002/(SICI)1521-4141(199907)29:07<2344::AID-IMMU2344>3.0.CO;2-R
10.1097/00062752-199801000-00002

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 9:27 p.m.)
Deposited	1 year, 10 months ago (Oct. 29, 2023, 1:35 p.m.)
Indexed	2 months, 2 weeks ago (June 23, 2025, 5:45 a.m.)
Issued	25 years, 6 months ago (March 1, 2000)
Published	25 years, 6 months ago (March 1, 2000)
Published Online	23 years, 8 months ago (Dec. 25, 2001)
Published Print	25 years, 6 months ago (March 1, 2000)

Funders 0

None

BibTeX

@article{Behboudi_2000, title={The effects of DNA containing CpG motif on dendritic cells}, volume={99}, ISSN={1365-2567}, url={http://dx.doi.org/10.1046/j.1365-2567.2000.00979.x}, DOI={10.1046/j.1365-2567.2000.00979.x}, number={3}, journal={Immunology}, publisher={Wiley}, author={Behboudi, S. and Chao, D. and Klenerman, P. and Austyn, J.}, year={2000}, month=mar, pages={361–366} }

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