DOI: 10.1046/j.1365-2443.2003.00613.x. Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE

Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE

10.1046/j.1365-2443.2003.00613.x

Crossref journal-article

Wiley

Genes to Cells (311)

Abstract

AbstractBackground: Basal transcription factors are essential for RNA polymerase II (RNAPII)‐catalysed transcription of many but not all the mRNA‐encoding genes in vivo as well as in vitro. For example, copper‐inducible transcription of the copper metallothionein gene CUP1 occurs independently of basal factor TFIIE in budding yeast. To gain insight into the mechanism by which the requirement for TFIIE is bypassed, we artificially recruited certain constituents of Mediator, a large protein complex transmitting signals from various activators to the RNAPII machinery, to the CUP1 promoter by protein fusions with Ace1, the copper‐inducible activator.Results: Fusions with Med2 or Pgd1 activated CUP1 independently of TFIIE. Surprisingly, fusions with neither Srb5 nor Med9 circumvented TFIIE requirement for the CUP1 activation. Components of TFIID were similarly recruited to the CUP1 promoter without activation. By using a chromatin immunoprecipitation technique, we found that TFIIE is necessary for stable binding of TFIIH and RNAPII to the ADH1 promoter, whose activation requires TFIIE. However, binding of TFIIH and RNAPII to CUP1 upon its activation did not require TFIIE.Conclusions: Our results strongly suggest that the TFIIE requirement of a gene is determined by a target(s) in Mediator through which the signal of the cognate activator is transmitted.

Bibliography

Sakurai, H., & Fukasawa, T. (2003). Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE. Genes to Cells, 8(1), 41â50. Portico.

Authors 2

Hiroshi Sakurai (first)
Toshio Fukasawa (additional)

References 41 Referenced 6

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 5:49 p.m.)
Deposited	1 year, 11 months ago (Aug. 29, 2023, 11:29 p.m.)
Indexed	1 year, 9 months ago (Oct. 31, 2023, 2:50 p.m.)
Issued	22 years, 7 months ago (Jan. 1, 2003)
Published	22 years, 7 months ago (Jan. 1, 2003)
Published Online	22 years, 7 months ago (Jan. 8, 2003)
Published Print	22 years, 7 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{Sakurai_2003, title={Artificial recruitment of certain Mediator components affects requirement of basal transcription factor IIE}, volume={8}, ISSN={1365-2443}, url={http://dx.doi.org/10.1046/j.1365-2443.2003.00613.x}, DOI={10.1046/j.1365-2443.2003.00613.x}, number={1}, journal={Genes to Cells}, publisher={Wiley}, author={Sakurai, Hiroshi and Fukasawa, Toshio}, year={2003}, month=jan, pages={41–50} }

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