DOI: 10.1046/j.1365-2249.1996.d01-800.x. Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells

Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells

10.1046/j.1365-2249.1996.d01-800.x

Crossref journal-article

Oxford University Press (OUP)

Clinical and Experimental Immunology (286)

Abstract

Abstract Glycophosphatidylinositol (GPI)-linked Thy-1 molecules, well known cell surface markers of murine T cells, are present on the glomerular mesangial cells of the rat kidney. The administration of anti-Thy-1.1 MoAbs 1-22-3 and OX-7 to rats induces severe and mild complement-dependent mesangial proliferative glomerulonephritis, respectively. In order to determine whether protein-tyrosine kinase (PTK) activity is associated with Thy-1 molecules on rat mesangial cell surface, we performed an immune complex kinase assay, using anti-Thy-1 MoAbs 1-22-3 and OX-7, followed by reimmunoprecipitation with anti-phosphotyrosine, anti-fyn, anti-lck and anti-lyn antibodies. Physical association of PTK, p59fyn and p56/53lyn with Thy-1 molecules was demonstated in cultured rat mesangial cells. The activities of these kinases detected in MoAb 1-22-3 precipitates were higher than those in MoAb OX-7 precipitates. These results suggest that Thy-1 molecule transduces some signals also in rat mesangial cells.

Bibliography

Narisawa-Saito, M., Yamanashi, Y., Morioka, T., Oite, T., & Shimizu, F. (1996). Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells. Clinical and Experimental Immunology, 106(1), 86â90.

Authors 5

M Narisawa-Saito (first)
Y Yamanashi (additional)
T Morioka (additional)
T Oite (additional)
F Shimizu (additional)

References 0 Referenced 13

None

Dates

Type	When
Created	21 years, 10 months ago (Nov. 6, 2003, 11 a.m.)
Deposited	2 years, 6 months ago (Feb. 14, 2023, 6:40 a.m.)
Indexed	1 year, 3 months ago (June 1, 2024, 4:24 a.m.)
Issued	28 years, 11 months ago (Oct. 1, 1996)
Published	28 years, 11 months ago (Oct. 1, 1996)
Published Online	21 years, 10 months ago (Oct. 29, 2003)
Published Print	21 years, 10 months ago (Oct. 29, 2003)

Funders 0

None

BibTeX

@article{Narisawa_Saito_1996, title={Thy-1 molecule associates with protein tyrosine kinase(s) in rat mesangial cells}, volume={106}, ISSN={0009-9104}, url={http://dx.doi.org/10.1046/j.1365-2249.1996.d01-800.x}, DOI={10.1046/j.1365-2249.1996.d01-800.x}, number={1}, journal={Clinical and Experimental Immunology}, publisher={Oxford University Press (OUP)}, author={Narisawa-Saito, M and Yamanashi, Y and Morioka, T and Oite, T and Shimizu, F}, year={1996}, month=oct, pages={86–90} }

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