DOI: 10.1046/j.1365-2222.2003.01550.x. Microarrayed recombinant allergens for diagnosis of allergy

Microarrayed recombinant allergens for diagnosis of allergy

10.1046/j.1365-2222.2003.01550.x

Crossref journal-article

Wiley

Clinical & Experimental Allergy (311)

Abstract

SummaryWe suggest that the coapplication of recombinant allergens and microarray technology can lead to the development of new forms of multi‐allergen tests which allow the determining and monitoring of complex sensitization profiles of allergic patients in single assays. The allergen extracts which have so far been used for diagnosis only allowed the determining of whether an allergic patient is sensitized against a particular allergen source, but the disease‐eliciting allergens could not be identified. Through the application of recombinant DNA technology a rapidly growing panel of recombinant allergen molecules has become available which meanwhile comprises the epitope spectrum of most of the important allergen sources. We demonstrate that microarray technology can be used to establish multi‐allergen tests consisting of microarrayed recombinant allergen molecules. Microarrayed recombinant allergens can be used to determine and monitor the profile of disease‐eliciting allergens using single tests that require minute amounts of serum from allergic patients. The wealth of diagnostic information gained through microarray‐based allergy testing will likely improve diagnosis, prevention and treatment of allergy.

Bibliography

Harwanegg, C., Laffer, S., Hiller, R., Mueller, M. W., Kraft, D., Spitzauer, S., & Valenta, R. (2003). Microarrayed recombinant allergens for diagnosis of allergy. Clinical & Experimental Allergy, 33(1), 7â13. Portico.

Authors 7

C. Harwanegg (first)
S. Laffer (additional)
R. Hiller (additional)
M. W. Mueller (additional)
D. Kraft (additional)
S. Spitzauer (additional)
R. Valenta (additional)

References 67 Referenced 116

{'key': 'e_1_2_7_2_2', 'volume-title': 'Allergy and Allergic Disease', 'author': 'Kay AB', 'year': '1997'} / Allergy and Allergic Disease by Kay AB (1997)
10.1038/35095579
10.4049/jimmunol.97.6.840 / J Immunol / Physicochemical properties of human reaginic antibody by Ishizaka K (1966)
{'key': 'e_1_2_7_5_2', 'first-page': '381', 'article-title': 'Immunological study of an atypical (myeloma) immunoglobulin', 'volume': '13', 'author': 'Johansson SGO', 'year': '1967', 'journal-title': 'Immunology'} / Immunology / Immunological study of an atypical (myeloma) immunoglobulin by Johansson SGO (1967)
10.1073/pnas.74.7.2993
10.1159/000237545
10.1159/000237499
10.1016/S0091-6749(75)80003-0
10.1007/BF00019185
10.1016/S0091-6749(96)80187-4
{'key': 'e_1_2_7_12_2', 'first-page': '54', 'article-title': 'In vivo and in vitro use of recombinant allergens', 'volume': '6', 'author': 'Bousquet J', 'year': '1994', 'journal-title': 'ACI Intern'} / ACI Intern / In vivo and in vitro use of recombinant allergens by Bousquet J (1994)
10.1016/S0091-6749(98)70271-4
10.1111/j.1365-2222.1995.tb00998.x
{'key': 'e_1_2_7_15_2', 'volume-title': 'Experimental Researches on the Causes and Nature of Catarrhus Aestivus (Hayfever or Hay‐Asthma).', 'author': 'Blackley CH', 'year': '1959'} / Experimental Researches on the Causes and Nature of Catarrhus Aestivus (Hayfever or Hay‐Asthma). by Blackley CH (1959)
{'key': 'e_1_2_7_16_2', 'first-page': '160', 'article-title': 'Studien über die Überempfindlichkeit', 'volume': '86', 'author': 'Prausnitz C', 'year': '1921', 'journal-title': 'Centralbe F Bakt 1 Abt Orig'} / Centralbe F Bakt 1 Abt Orig / Studien über die Überempfindlichkeit by Prausnitz C (1921)
10.4049/jimmunol.99.6.1187 / J Immunol / Identification of gamma‐E antibodies as a carrier of reaginic activity by Ishizaka K (1967)
10.1111/j.1399-3038.2008.00827.x
10.1007/BF02992996
10.1016/0091-6749(87)90128-X
{'key': 'e_1_2_7_21_2', 'first-page': '127', 'article-title': 'Value of monoclonal antibody‐based assay: advantages and drawbacks', 'volume': '87', 'author': 'Van Ree R', 'year': '1994', 'journal-title': 'Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M'} / Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf A M / Value of monoclonal antibody‐based assay: advantages and drawbacks by Van Ree R (1994)
10.1016/0952-7915(95)80043-3
10.1046/j.1365-2222.1999.00653.x
10.1046/j.1365-2222.1999.00670.x
10.1067/mai.2000.109832
10.1016/S0091-6749(98)70273-8
10.1016/0091-6749(91)90245-J
10.1159/000236135
10.1016/S0091-6749(96)70099-4
10.1016/S0091-6749(98)70391-4
10.1159/000024278
10.1159/000053815
10.1159/000023889
10.1159/000024440
10.1159/000024443
10.1016/0091-6749(76)90149-4
10.1016/S0091-6749(95)70096-X
10.1016/0091-6749(92)90465-E
10.1046/j.1365-2222.1999.00529.x
10.1111/j.1432-1033.1995.484_2.x
10.1074/jbc.M101657200
10.1006/bbrc.1995.2320
10.1111/j.1398-9995.1998.tb03771.x
10.1159/000023932
10.1034/j.1398-9995.2002.13248.x
10.1159/000057742
10.1515/BC.1999.101
10.1159/000024181
{'key': 'e_1_2_7_49_2', 'first-page': '495', 'volume-title': 'Inflammatory Mechanisms. Clinical Allergy and Immunology', 'author': 'Valenta R', 'year': '2001'} / Inflammatory Mechanisms. Clinical Allergy and Immunology by Valenta R (2001)
10.1016/S0091-6749(99)70077-1
10.1046/j.1365-2222.2000.00869.x
10.1067/mai.2000.106924
10.1016/S0091-6749(99)70019-9
10.1038/nri824
10.1096/fj.01-0711fje
10.1126/science.270.5235.467
10.1126/science.289.5485.1760
10.1126/science.1062191
10.1186/gb-2001-2-2-research0004
10.1038/45350
10.1023/A:1026330015280
10.1093/nar/26.21.5007
10.1006/abio.1999.4063
10.1093/nar/28.2.e3
10.1038/nm0302-295
10.1016/j.clinbiochem.2004.06.010
10.1093/clinchem/44.9.2015
10.1073/pnas.170237197

Dates

Type	When
Created	22 years, 5 months ago (March 11, 2003, 4:53 a.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 7:21 p.m.)
Indexed	5 months, 1 week ago (March 19, 2025, 12:51 p.m.)
Issued	22 years, 7 months ago (Jan. 1, 2003)
Published	22 years, 7 months ago (Jan. 1, 2003)
Published Online	22 years, 7 months ago (Jan. 14, 2003)
Published Print	22 years, 7 months ago (Jan. 1, 2003)

Funders 0

None

BibTeX

@article{Harwanegg_2003, title={Microarrayed recombinant allergens for diagnosis of allergy}, volume={33}, ISSN={1365-2222}, url={http://dx.doi.org/10.1046/j.1365-2222.2003.01550.x}, DOI={10.1046/j.1365-2222.2003.01550.x}, number={1}, journal={Clinical & Experimental Allergy}, publisher={Wiley}, author={Harwanegg, C. and Laffer, S. and Hiller, R. and Mueller, M. W. and Kraft, D. and Spitzauer, S. and Valenta, R.}, year={2003}, month=jan, pages={7–13} }

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