DOI: 10.1046/j.0953-816x.2001.01565.x. Identification of a <i>cis</i>‐acting dendritic targeting element in the mRNA encoding the alpha subunit of Ca<sup>2+</sup>/calmodulin‐dependent protein kinase II

Identification of a cis‐acting dendritic targeting element in the mRNA encoding the alpha subunit of Ca2+/calmodulin‐dependent protein kinase II

10.1046/j.0953-816x.2001.01565.x

Crossref journal-article

Wiley

European Journal of Neuroscience (311)

Abstract

AbstractIn mammalian neurons a selected group of mRNAs, including the transcript encoding the alpha subunit of Ca2+/calmodulin‐dependent protein kinase II, is found in dendrites. The molecular mechanisms underlying extrasomatic RNA trafficking are not well described. It is thought that dendritic transcripts contain cis‐acting elements that direct their selective subcellular sorting. Here we report the identification of an extrasomatic targeting element in the 3′ untranslated region of the mRNA encoding the alpha subunit of Ca2+/calmodulin‐dependent protein kinase II. In primary hippocampal neurons, this 1200‐nucleotide‐spanning, cis‐acting element is sufficient to mediate dendritic localization of chimeric reporter transcripts. The trafficking signal does not share any striking sequence similarity with a previously characterized dendritic targeting element in transcripts encoding the microtubule‐associated protein 2. In dendrites of transfected primary neurons, recombinant RNAs form granules with an average diameter of 0.45 µm that may represent preferential RNA docking sites or multimolecular transport units. These findings imply that extrasomatic sorting of individual dendritic mRNAs involves at least partially distinct molecular mechanisms, as well as large trafficking complexes.

Bibliography

Blichenberg, A., Rehbein, M., MÃ¼ller, R., Garner, C. C., Richter, D., & Kindler, S. (2001). Identification of a cisâacting dendritic targeting element in the mRNA encoding the alpha subunit of Ca2+/calmodulinâdependent protein kinase II. European Journal of Neuroscience, 13(10), 1881â1888. Portico.

Authors 6

Arne Blichenberg (first)
Monika Rehbein (additional)
Reinhold Müller (additional)
Craig C. Garner (additional)
Dietmar Richter (additional)
Stefan Kindler (additional)

References 50 Referenced 85

10.1091/mbc.10.9.2945
10.1083/jcb.123.2.431
10.1523/JNEUROSCI.18-01-00251.1998
10.1523/JNEUROSCI.19-20-08818.1999 / J. Neurosci. / Identification of a cis‐acting dendritic targeting element in MAP2 mRNAs by Blichenberg A. (1999)
10.1016/0896-6273(90)90340-L
10.1016/0896-6273(88)90210-3
10.1523/JNEUROSCI.10-06-01788.1990
10.1016/S0960-9822(99)80144-4
10.1523/JNEUROSCI.13-09-04054.1993
10.1016/S0896-6273(00)80248-2
10.1038/336674a0
{'key': 'e_1_2_6_13_1', 'first-page': '251', 'volume-title': 'Rat Hippocampal Neurons in Low Density Culture', 'author': 'Goslin K.', 'year': '1991'} / Rat Hippocampal Neurons in Low Density Culture by Goslin K. (1991)
10.1016/S0959-437X(96)80059-1
10.1126/science.3037704
10.1016/S0092-8674(00)81613-X
10.1006/mcne.1996.0594
10.1126/science.273.5280.1402
10.1016/0169-328X(95)00237-M
10.1016/S0303-7207(97)04015-X
10.1016/0896-6273(90)90341-C
10.1016/0169-328X(93)90057-V
10.1523/JNEUROSCI.16-24-07812.1996
10.1016/S0959-4388(98)80087-1
10.1073/pnas.84.16.5962
10.1073/pnas.92.12.5734
10.1016/0896-6273(95)90299-6
10.1073/pnas.93.23.13250
10.1016/S0960-9822(06)00287-9
10.1016/S0021-9258(17)39454-1 / J. Biol. Chem. / Distinct forbrain and cerebellar isozymes of type II Ca2+/calmodulin‐ dependent protein kinase associate differently with the postsynaptic density fraction by Miller S.G. (1985)
10.1016/S0301-0082(98)00066-5
10.1073/pnas.92.10.4377
10.1038/80591
10.1096/fasebj.13.3.435
10.1523/JNEUROSCI.17-12-04722.1997
10.1523/JNEUROSCI.19-18-07823.1999
10.1111/j.1460-9568.1997.tb01629.x
10.1111/j.1471-4159.1993.tb03594.x
10.1016/S0169-328X(00)00114-5
10.1523/JNEUROSCI.20-17-06385.2000
10.1016/S0896-6273(01)80041-6
10.1523/JNEUROSCI.19-18-07834.1999 / J. Neurosci. / Lamina‐specific synaptic activation causes domain‐specific alterations in dendritic immunostaining for MAP2 and CAM kinase II by Steward O. (1999)
10.1523/JNEUROSCI.02-03-00284.1982
10.1523/JNEUROSCI.08-01-00176.1988 / J. Neurosci. / Protein‐synthetic machinery beneath postsynaptic sites on CNS neurons: association between polyribosomes and other organelles at the synaptic site by Steward O. (1988)
10.1016/S0896-6273(00)80591-7
10.1126/science.283.5399.186
10.1523/JNEUROSCI.16-22-07171.1996
10.1523/JNEUROSCI.12-03-00762.1992
10.1523/JNEUROSCI.18-01-00026.1998
10.1016/S0959-4388(99)00050-1
10.1016/S0896-6273(00)80630-3

Dates

Type	When
Created	22 years, 5 months ago (March 6, 2003, 9:35 a.m.)
Deposited	1 year, 11 months ago (Oct. 2, 2023, 7:46 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 8, 2024, 8:31 p.m.)
Issued	24 years, 4 months ago (May 1, 2001)
Published	24 years, 4 months ago (May 1, 2001)
Published Online	23 years, 8 months ago (Dec. 20, 2001)
Published Print	24 years, 4 months ago (May 1, 2001)

Funders 0

None

BibTeX

@article{Blichenberg_2001, title={Identification of a cis‐acting dendritic targeting element in the mRNA encoding the alpha subunit of Ca2+/calmodulin‐dependent protein kinase II}, volume={13}, ISSN={1460-9568}, url={http://dx.doi.org/10.1046/j.0953-816x.2001.01565.x}, DOI={10.1046/j.0953-816x.2001.01565.x}, number={10}, journal={European Journal of Neuroscience}, publisher={Wiley}, author={Blichenberg, Arne and Rehbein, Monika and Müller, Reinhold and Garner, Craig C. and Richter, Dietmar and Kindler, Stefan}, year={2001}, month=may, pages={1881–1888} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        9,
        9
      ]
    ],
    "date-time": "2024-09-09T00:31:33Z",
    "timestamp": 1725841893947
  },
  "reference-count": 50,
  "publisher": "Wiley",
  "issue": "10",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2001,
            12,
            20
          ]
        ],
        "date-time": "2001-12-20T00:00:00Z",
        "timestamp": 1008806400000
      },
      "content-version": "vor",
      "delay-in-days": 233,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [
      "onlinelibrary.wiley.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>In mammalian neurons a selected group of mRNAs, including the transcript encoding the alpha subunit of Ca<jats:sup>2+</jats:sup>/calmodulin\u2010dependent protein kinase II, is found in dendrites. The molecular mechanisms underlying extrasomatic RNA trafficking are not well described. It is thought that dendritic transcripts contain <jats:italic>cis</jats:italic>\u2010acting elements that direct their selective subcellular sorting. Here we report the identification of an extrasomatic targeting element in the 3\u2032 untranslated region of the mRNA encoding the alpha subunit of Ca<jats:sup>2+</jats:sup>/calmodulin\u2010dependent protein kinase II. In primary hippocampal neurons, this 1200\u2010nucleotide\u2010spanning, <jats:italic>cis</jats:italic>\u2010acting element is sufficient to mediate dendritic localization of chimeric reporter transcripts. The trafficking signal does not share any striking sequence similarity with a previously characterized dendritic targeting element in transcripts encoding the microtubule\u2010associated protein 2. In dendrites of transfected primary neurons, recombinant RNAs form granules with an average diameter of 0.45\u2003\u00b5m that may represent preferential RNA docking sites or multimolecular transport units. These findings imply that extrasomatic sorting of individual dendritic mRNAs involves at least partially distinct molecular mechanisms, as well as large trafficking complexes.</jats:p>",
  "DOI": "10.1046/j.0953-816x.2001.01565.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        3,
        6
      ]
    ],
    "date-time": "2003-03-06T14:35:55Z",
    "timestamp": 1046961355000
  },
  "page": "1881-1888",
  "update-policy": "http://dx.doi.org/10.1002/crossmark_policy",
  "source": "Crossref",
  "is-referenced-by-count": 85,
  "title": "Identification of a <i>cis</i>\u2010acting dendritic targeting element in the mRNA encoding the alpha subunit of Ca<sup>2+</sup>/calmodulin\u2010dependent protein kinase II",
  "prefix": "10.1111",
  "volume": "13",
  "author": [
    {
      "given": "Arne",
      "family": "Blichenberg",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Monika",
      "family": "Rehbein",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Reinhold",
      "family": "M\u00fcller",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Craig C.",
      "family": "Garner",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Dietmar",
      "family": "Richter",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Stefan",
      "family": "Kindler",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2001,
        12,
        20
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.10.9.2945"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.123.2.431"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-01-00251.1998"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "8818",
      "DOI": "10.1523/JNEUROSCI.19-20-08818.1999",
      "article-title": "Identification of a cis\u2010acting dendritic targeting element in MAP2 mRNAs",
      "volume": "19",
      "author": "Blichenberg A.",
      "year": "1999",
      "journal-title": "J. Neurosci."
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(90)90340-L"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(88)90210-3"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.10-06-01788.1990"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(99)80144-4"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.13-09-04054.1993"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80248-2"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/336674a0"
    },
    {
      "key": "e_1_2_6_13_1",
      "first-page": "251",
      "volume-title": "Rat Hippocampal Neurons in Low Density Culture",
      "author": "Goslin K.",
      "year": "1991"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-437X(96)80059-1"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.3037704"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(00)81613-X"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/mcne.1996.0594"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.273.5280.1402"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0169-328X(95)00237-M"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0303-7207(97)04015-X"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(90)90341-C"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0169-328X(93)90057-V"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.16-24-07812.1996"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(98)80087-1"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.84.16.5962"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.12.5734"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0896-6273(95)90299-6"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.23.13250"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0960-9822(06)00287-9"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "crossref",
      "first-page": "9039",
      "DOI": "10.1016/S0021-9258(17)39454-1",
      "article-title": "Distinct forbrain and cerebellar isozymes of type II Ca2+/calmodulin\u2010 dependent protein kinase associate differently with the postsynaptic density fraction",
      "volume": "260",
      "author": "Miller S.G.",
      "year": "1985",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0301-0082(98)00066-5"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.92.10.4377"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/80591"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1096/fasebj.13.3.435"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.17-12-04722.1997"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.19-18-07823.1999"
    },
    {
      "key": "e_1_2_6_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1460-9568.1997.tb01629.x"
    },
    {
      "key": "e_1_2_6_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1471-4159.1993.tb03594.x"
    },
    {
      "key": "e_1_2_6_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0169-328X(00)00114-5"
    },
    {
      "key": "e_1_2_6_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.20-17-06385.2000"
    },
    {
      "key": "e_1_2_6_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(01)80041-6"
    },
    {
      "key": "e_1_2_6_42_1",
      "doi-asserted-by": "crossref",
      "first-page": "7834",
      "DOI": "10.1523/JNEUROSCI.19-18-07834.1999",
      "article-title": "Lamina\u2010specific synaptic activation causes domain\u2010specific alterations in dendritic immunostaining for MAP2 and CAM kinase II",
      "volume": "19",
      "author": "Steward O.",
      "year": "1999",
      "journal-title": "J. Neurosci."
    },
    {
      "key": "e_1_2_6_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.02-03-00284.1982"
    },
    {
      "key": "e_1_2_6_44_1",
      "doi-asserted-by": "crossref",
      "first-page": "176",
      "DOI": "10.1523/JNEUROSCI.08-01-00176.1988",
      "article-title": "Protein\u2010synthetic machinery beneath postsynaptic sites on CNS neurons: association between polyribosomes and other organelles at the synaptic site",
      "volume": "8",
      "author": "Steward O.",
      "year": "1988",
      "journal-title": "J. Neurosci."
    },
    {
      "key": "e_1_2_6_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80591-7"
    },
    {
      "key": "e_1_2_6_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.283.5399.186"
    },
    {
      "key": "e_1_2_6_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.16-22-07171.1996"
    },
    {
      "key": "e_1_2_6_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.12-03-00762.1992"
    },
    {
      "key": "e_1_2_6_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1523/JNEUROSCI.18-01-00026.1998"
    },
    {
      "key": "e_1_2_6_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0959-4388(99)00050-1"
    },
    {
      "key": "e_1_2_6_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0896-6273(00)80630-3"
    }
  ],
  "container-title": "European Journal of Neuroscience",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.0953-816x.2001.01565.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.0953-816x.2001.01565.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        2
      ]
    ],
    "date-time": "2023-10-02T23:46:36Z",
    "timestamp": 1696290396000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1046/j.0953-816x.2001.01565.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "references-count": 50,
  "journal-issue": {
    "issue": "10",
    "published-print": {
      "date-parts": [
        [
          2001,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1046/j.0953-816x.2001.01565.x"
  ],
  "URL": "http://dx.doi.org/10.1046/j.0953-816x.2001.01565.x",
  "relation": {},
  "ISSN": [
    "0953-816X",
    "1460-9568"
  ],
  "subject": [],
  "container-title-short": "Eur J of Neuroscience",
  "published": {
    "date-parts": [
      [
        2001,
        5
      ]
    ]
  },
  "assertion": [
    {
      "value": "2001-12-20",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}