DOI: 10.1042/bst0300133. Functions and interplay of the tRNA-binding sites of the ribosome

Functions and interplay of the tRNA-binding sites of the ribosome

10.1042/bst0300133

Crossref journal-article

Portland Press Ltd.

Biochemical Society Transactions (288)

Abstract

The ribosome translates the genetic information of an mRNA molecule into a sequence of amino acids. The ribosome utilizes tRNAs to connect elements of the RNA and protein worlds during protein synthesis, i.e. an anticodon as a unit of genetic information with the corresponding amino acid as a building unit of proteins. Three tRNA-binding sites are located on the ribosome, termed the A, P and E sites. In recent years the tRNA-binding sites have been localized on the ribosome by three different techniques, small-angle neutron scattering, cryo-electron microscopy and X-ray analyses of 70 S crystals. These high-resolution glimpses into various ribosomal states together with a large body of biochemical data reveal an intricate interplay between the tRNAs and the three ribosomal binding sites, providing an explanation for the remarkable features of the ribosome, such as the ability to select the correct ternary complex aminoacyl-tRNA · EF-Tu · GTP out of more than 40 extremely similar tRNA complexes, the precise movement of the tRNA2 · mRNA complex during translocation and the maintenance of the reading frame.

Bibliography

MÃ¡rquez, V., Wilson, D. N., & Nierhaus, K. H. (2002). Functions and interplay of the tRNA-binding sites of the ribosome. Biochemical Society Transactions, 30(2), 133â140.

Authors 3

V. Márquez (first)
D. N. Wilson (additional)
K. H. Nierhaus (additional)

References 0 Referenced 8

None

Dates

Type	When
Created	20 years, 7 months ago (Jan. 31, 2005, 7:57 a.m.)
Deposited	3 years, 9 months ago (Nov. 17, 2021, 2:59 p.m.)
Indexed	1 year, 11 months ago (Sept. 13, 2023, 12:55 p.m.)
Issued	23 years, 5 months ago (April 1, 2002)
Published	23 years, 5 months ago (April 1, 2002)
Published Print	23 years, 5 months ago (April 1, 2002)

Funders 0

None

BibTeX

@article{M_rquez_2002, title={Functions and interplay of the tRNA-binding sites of the ribosome}, volume={30}, ISSN={1470-8752}, url={http://dx.doi.org/10.1042/bst0300133}, DOI={10.1042/bst0300133}, number={2}, journal={Biochemical Society Transactions}, publisher={Portland Press Ltd.}, author={Márquez, V. and Wilson, D. N. and Nierhaus, K. H.}, year={2002}, month=apr, pages={133–140} }

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