DOI: 10.1042/bj3400593. Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

10.1042/bj3400593

Crossref journal-article

Portland Press Ltd.

Biochemical Journal (288)

Abstract

We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two polyclonal antibodies raised against distinct regions of the Xtrp sequence revealed Xtrp expression in various Xenopus tissues, and the localization of Xtrp at the plasma membrane of Xenopus oocytes and HeLa cells. Since capacitative calcium entry into Xenopus oocytes has been shown previously to be substantially inhibited by trp1 antisense oligonucleotides [Tomita, Kaneko, Funayama, Kondo, Satoh and Akaike (1998) Neurosci. Lett. 248, 195-198] we suggest that Xtrp may underlie capacitative calcium entry in Xenopus tissues.

Bibliography

BOBANOVIÄ, L. K., LAINE, M., PETERSEN, C. C. H., BENNETT, D. L., BERRIDGE, M. J., LIPP, P., RIPLEY, S. J., & BOOTMAN, M. D. (1999). Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus. Biochemical Journal, 340(3), 593â599.

Authors 8

Laura K. BOBANOVIĆ (first)
Mika LAINE (additional)
Carl C. H. PETERSEN (additional)
Deborah L. BENNETT (additional)
Michael J. BERRIDGE (additional)
Peter LIPP (additional)
Scott J. RIPLEY (additional)
Martin D. BOOTMAN (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	10 years ago (Aug. 10, 2015, 6:06 p.m.)
Deposited	3 years, 9 months ago (Nov. 23, 2021, 7:47 p.m.)
Indexed	3 months, 1 week ago (May 20, 2025, 9:18 a.m.)
Issued	26 years, 2 months ago (June 8, 1999)
Published	26 years, 2 months ago (June 8, 1999)
Published Online	26 years, 2 months ago (June 8, 1999)
Published Print	26 years, 2 months ago (June 15, 1999)

Funders 0

None

BibTeX

@article{BOBANOVI__1999, title={Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus}, volume={340}, ISSN={1470-8728}, url={http://dx.doi.org/10.1042/bj3400593}, DOI={10.1042/bj3400593}, number={3}, journal={Biochemical Journal}, publisher={Portland Press Ltd.}, author={BOBANOVIĆ, Laura K. and LAINE, Mika and PETERSEN, Carl C. H. and BENNETT, Deborah L. and BERRIDGE, Michael J. and LIPP, Peter and RIPLEY, Scott J. and BOOTMAN, Martin D.}, year={1999}, month=jun, pages={593–599} }

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